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Method for adjusting and controlling mixed culture of floating microalgae by bacillus

A technology of mixed culture and Bacillus, applied in the field of planktonic microalgae cultivation, can solve the problems of difficult cultivation of algae phase, easy mutation, unfavorable microalgae community stability, etc., and achieve the effect of ensuring diversity and stability

Inactive Publication Date: 2009-09-16
SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The stability of the microalgae community structure in the water body environment is not only related to the biomass of the microalgae, but also closely related to its community diversity index. At present, the regulation of microalgae or the artificial directional cultivation of planktonic microalgae are mostly aimed at a single species or two excellent species. The development of microalgae, in a complex water environment, is likely to lead to excessive reproduction of individual microalgae species at a certain stage, and the diversity index of the microalgae community in the water body is relatively low, which is not conducive to the stability of the microalgae community in the water environment. In the early stage, the algal phase is difficult to cultivate, in the middle stage, it is easy to mutate, and in the later stage, it is too vigorous

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Nannochloropsis oculata, Cryptomonas erosa and Oscillatoria chloride were selected for mixed culture, and Bacillus licheniformis was used to control the culture process, so that various microalgae in the system were A certain percentage of steady growth.

[0024] The culture medium is fully artificially prepared inorganic culture medium, and the old sea water is boiled and cooled and then added with NaNO 3 、KH 2 PO 4 and ferric citrate, so that the concentration of N, P and Fe reached 15mg / L, 2mg / L and 0.12mg / L respectively. During the cultivation process, measure the concentration of nitrogen and phosphorus every 3 days, and add NaNO as appropriate 3 and KH 2 PO 4 , so that the concentration of total nitrogen and phosphorus reached the initial level.

[0025] During the cultivation process, the relevant conditions of each culture group were consistent. The temperature was controlled by a constant temperature circulator water bath, the temperature was 26°C, the pH...

Embodiment 2

[0030] Nannochloropsis oculata, Cryptomonas erosa and Oscillatoria chloride were selected for mixed culture, and Bacillus licheniformis was used to control the culture process, so that various microalgae in the system were A certain percentage of steady growth.

[0031] The culture medium is fully artificially prepared inorganic culture medium, and the old sea water is boiled and cooled and then added with NaNO 3 、KH 2 PO 4 and ferric citrate, so that the concentration of N, P and Fe reached 16mg / L, 3mg / L and 0.14mg / L respectively. During the cultivation process, measure the concentration of nitrogen and phosphorus every 3 days, and add NaNO as appropriate 3 and KH 2 PO 4 , so that the concentration of total nitrogen and phosphorus reached the initial level.

[0032] During the cultivation process, the relevant conditions of each culture group were consistent. The temperature was controlled by a constant temperature circulator water bath, the temperature was 28°C, the pH...

Embodiment 3

[0037] Select the water color of the pond as bright green, bean green or green-brown, the phytoplankton microalgae biodiversity index of the aquaculture water body is greater than 1.60, and the shrimp mariculture ponds with excellent production performance are followed and analyzed throughout the whole process, and the Nannochloropsis with a dominance degree greater than 0.15 ( Nannochloropsis oculata), Cryptomonas erosa, and Oscillatoria chloride were mixed cultured, and Bacillus licheniformis was used to control the growth of various microalgae in the system in a certain proportion. During the cultivation process, simple correlation analysis was used to analyze the correlation between bacillus and various microalgae cell growth; path analysis was used to evaluate the influence of bacillus on various microalgae.

[0038] The culture medium is fully artificially prepared inorganic culture medium, and the old sea water is boiled and cooled and then added with KNO 3 , Ca(H 2 PO...

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Abstract

The invention discloses a method for adjusting and controlling mixed culture of floating microalgae by bacillus. The microalgae, the dominance degree of which is more than 0.15, is selected and placed into a culture solution to carry out mixed culture, wherein in the culture solution, the concentration of nitrogen is 12 to 16 mg / L, the concentration of phosphorus is 2 to 6 mg / L and the concentration of iron is 0.10 to 0.20 mg / L; during the mixed culture, the Bacillus licheniformis is adopted for adjustment and control; and the culture conditions comprise that the temperature is between 26 and 28 DEG C, the salinity is 12 to 18 per mill, the pH is 7.5 to 8.0, the illumination intensity is 4,000 to 5,000Lx, and the ratio of light to darkness is 12 hours to 12 hours and 14 hours to 10 hours. By adopting the method for carrying out the mixed culture on the microalgae by using the bacillus in a mixed culture system, the growth of the microalgae is balanced, the stability is good, single biological diversity of algae phases in the mixed culture system can be avoided, and extreme advantages or disadvantages on the number of the microalgae can be avoided.

Description

technical field [0001] The invention relates to a method for cultivating planktonic microalgae, in particular to a method for regulating the mixed cultivation of planktonic microalgae by Bacillus. Background technique [0002] The stability of the microalgae community structure in the water body environment is not only related to the biomass of the microalgae, but also closely related to its community diversity index. At present, the regulation of microalgae or the artificial directional cultivation of planktonic microalgae are mostly aimed at a single species or two excellent species. The development of microalgae, in a complex water environment, is likely to lead to excessive reproduction of individual microalgae species at a certain stage, and the diversity index of the microalgae community in the water body is relatively low, which is not conducive to the stability of the microalgae community in the water environment. In the early stage, the algal phase is difficult to c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/12C12R1/10
Inventor 曹煜成李卓佳王少沛文国樑杨莺莺
Owner SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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