Solid-state fermentation preparation method of lotus root dietary fiber and products thereof
A solid-state fermentation and dietary fiber technology, applied in the fields of health food production and food biology, can solve the problems of environmental pollution, loss of effective components of soluble dietary fiber, etc., and achieve the effect of strong operability, low price, and improvement of physiological functions.
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Embodiment 1
[0047] Use lotus root powder or lotus root juice beverages and other products produced during the processing of lotus root dregs as the main raw material, dried, crushed by a grinder, and then passed through a 60-mesh sieve. Add distilled water at a material-to-liquid ratio of 1:1.5. Sterilize at 121°C for 20 minutes. After cooling, stir in 1.5% Angel rice koji, and saccharify at 28-30°C for 36 hours to serve as a solid culture medium. Take the Korean Ganodermalucidum (Ganodermalucidum HG) preserved strain and inoculate it in a petri dish containing an activation medium as a potato potato medium. After incubating at 28~30℃ for 5-6 days, use a sterile punch to take the diameter on the petri dish 4 slices of 1cm hyphae, inoculated into a 250ml Erlenmeyer flask containing 50ml mother seed culture medium, cultured at 28~30℃ and 120rpm for 5-6 days to obtain female seed; mother seed medium is potato potato Liquid culture medium (reference: Zhang Song. Edible Mycology, East China Uni...
Embodiment 2
[0051] According to the raw material processing and sterilization method in Example 1, after cooling down, stir in 2% Angel rice koji, and saccharify at 28-30°C for 36 hours to serve as a solid culture substrate.
[0052] The mother seed culture method and the composition of the fermentation medium were the same as in Example 1. The seed solution was inoculated at 20% of the weight of the raw material, and cultured at 28-30°C for 7 days.
[0053] The lotus root dietary fiber is prepared by the well-known activation processes such as heating and drying, extrusion and puffing, and ultrafine pulverization. The output of insoluble dietary fiber in the product is 50.2g / 100g, and the output of soluble dietary fiber is 12.0g / 100g.
Embodiment 3
[0055] According to the raw material processing and sterilization method in Example 1, after cooling, stir in 1.8% Angel rice koji, and saccharify at 28-30°C for 36 hours to serve as a solid culture substrate.
[0056] The mother seed culture method and the composition of the fermentation medium were the same as in Example 1. The seed solution was inoculated at 18% of the weight of the raw material and cultured at 28-30°C for 7 days.
[0057] The lotus root dietary fiber is prepared by the well-known activation processes such as heating and drying, extrusion and puffing, and ultrafine pulverization. The output of insoluble dietary fiber in the product is 50.5g / 100g, and the output of soluble dietary fiber is 11.4g / 100g.
[0058] Table 1 Plackett-Burman experimental design and measurement data
[0059]
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