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Method and device for producing n-butyric acid by microbial catalysis

A technology of n-butyric acid and microorganisms, which is applied in the field of microbial catalytic production of n-butyric acid, can solve the problems of microbial toxicity and low extraction efficiency of extractants, and achieve the effects of reducing toxicity, prolonging effective running time, and avoiding direct contact

Active Publication Date: 2009-07-22
GUANGDONG INST OF MICROORGANISM
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  • Abstract
  • Description
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Problems solved by technology

[0008] Although the above-mentioned understandings and developments have been obtained, there are still several problems unsolved in the production of butyric acid by clostridium catalysis: (1) continuous fermentation and production of butyric acid cannot be realized while maintaining a high conversion rate, (2) The extractant has low extraction efficiency and is toxic to microorganisms

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  • Method and device for producing n-butyric acid by microbial catalysis

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Embodiment 1

[0033] Embodiment 1 The device for the production of n-butyric acid by microbial catalysis of the present invention

[0034] See attached figure 1 , the device for fermenting and producing n-butyric acid of the present invention comprises: fermenter 1, immobilization reactor 2, extracting device 3, stripping device 4, stripping agent receiving tank 5, salting-out tank 6, and storage tank 7, acid Alkali bottle 8, bacterial liquid separation device 9, extractant storage tank 10, stripping agent storage tank 11 and the pipelines, pump valves, etc. connecting them.

[0035] The immobilization reactor is a closed container filled with an immobilization carrier in a folded manner, and the immobilization carrier is composed of an immobilization carrier material wound on a support. The support can be sheet or mesh stainless steel or glass; the immobilized carrier material can be fibers such as cotton cloth, etc., or synthetic high molecular polymers such as polyvinyl alcohol, etc.

...

Embodiment 2

[0037] Embodiment 2 The method for the production of n-butyric acid catalyzed by microorganisms of the present invention

[0038] Proceed as follows:

[0039] (1) Preparation of seed solution

[0040] After the seed medium is bottled, cover it with a layer of liquid paraffin about 5-10 mm thick, sterilize it under high pressure at 121°C for 20 minutes, inoculate Clostridium tyrobutyricum, and culture it statically at 35-37°C for 48 hours to obtain the seed liquid for later use .

[0041] The formula of the seed medium is as follows: glucose 10g / L, peptone 5g / L, yeast extract 5g / L, beef extract 5g / L, ammonium nitrate 1g / L, ferrous sulfate 0.03g / L, sodium acetate 3g / L, chloride Sodium chloride 5g / L, soluble starch 1g / L, cysteine ​​0.5g / L, pH7.0.

[0042] (2) Preparation of microbial suspension

[0043] Put the Clostridium culture medium into the fermenter, and sterilize it under high pressure at 121°C for 25 minutes. After cooling, nitrogen gas is introduced to reduce the ox...

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Abstract

The invention discloses a method for producing ethyl acetic acid catalytically by microorganism and a device thereof. The method comprises the steps: preparation of a microbial suspension, immobilization of microbial cells, immobilized clostridium catalytic production of ethyl acetic acid, online separation of ethyl acetic acid and extraction. The device for producing the ethyl acetic acid by the microorganism comprises a fermentation flask, an immobilization reactor, an extracting device and a back-extracting device. The method and the device effectively relieve the inhibitive effect of final products, improve online extraction efficiency, lower the microbial toxicity of extracting agents, realize continuous production of the ethyl acetic acid and lead catalytic production of ethyl acetic acid by the microorganism to be developed towards industrialized direction.

Description

technical field [0001] The invention relates to the technical field of bioengineering and fermentation, in particular to a method and device for producing n-butyric acid by microorganism catalysis. Background technique [0002] Butyric acid (CH 3 CH 2 CH 2 COOH, butyrate, hereinafter referred to as butyric acid) has many uses in industry. It is used as a chemical raw material to manufacture cellulose butyrate and synthesize butyrate; in the food industry, it can be directly added to dairy products to improve the flavor of cream. Its esters are also widely used as fragrances in food and perfume. At the same time, butyric acid, as a short-chain fatty acid (short-chainfatty acid, SCFA), is the preferred energy source for human and other monogastric animal intestinal epithelial cells, which can inhibit the occurrence of enteritis, maintain and promote the recovery of normal intestinal function (Gibson, et al. al. Carcinogenesis 1999, 20: 539-544; Hijova, et al. Bratisl Lek L...

Claims

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Application Information

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IPC IPC(8): C12P7/52C12M1/00C12R1/145
Inventor 朱红惠邓名荣王燕郭俊李良秋
Owner GUANGDONG INST OF MICROORGANISM
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