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Culture medium for tissue engineering skin convey and formulating method thereof

A technology for tissue engineering skin and medium, applied in tissue culture, biochemical equipment and methods, artificial cell constructs, etc., to achieve the effects of simple preparation method, convenient synthesis and large-scale production, and long shelf life

Inactive Publication Date: 2009-07-22
CHINESE ACAD OF INSPECTION & QUARANTINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

After searching, there is no public information about the culture medium that has high stability, easy operation, good tissue culture effect and can effectively maintain the structure and function of tissue-engineered skin during transportation.

Method used

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Embodiment Construction

[0044] To further illustrate the present invention, it will be specifically described in conjunction with the following embodiments:

[0045] 1. The preparation of the culture medium for tissue engineering skin transportation according to the present invention:

[0046] 1. The components and the dosage of each component are:

[0047] (1) Basic culture medium: It is mixed with the following substances in volume percentage:

[0048] DMEM culture medium 65%

[0049] F12 medium 25%

[0050] Fetal bovine serum 10%

[0051] (2) Nutrients:

[0052]Hydrocortisone 0.4~0.5μg / mL

[0053] Epidermal growth factor 5~15ng / mL

[0054] Isoproterenol 1~3×10 -6 mol / L

[0055] Insulin 5~15μg / mL

[0056] Transferrin 5~10μg / mL

[0057] Triiodothyronine 2~6×10 -9 mol / L

[0058] Adenine 1~3×10 -4 mol / L

[0059] Vitamin C 50~100μg / mL

[0060] Cholera toxin 0.1~1nmol / L

[0061] L-serine 1~3×10 -2 mol / L

[0062] L-carnitine 1~5×10 -5 mol / L

[0063] Palmitic acid 15~35μmol / L

[0064] Linoleic acid 5~25μmol / L...

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Abstract

The invention discloses a culture medium that is used in transportation of tissue engineered skin. A basic culture fluid is added with additional hydrocortisone, epidermal growth factors, isoproterenol, insulin, transferrin, triiodothyronine, adenine, vitamin C, cholera toxin, L-serine, L-novain, palmic acid, linoleic acid, arachidonic acid, bovine pituitary extract, bovine serum albumin, penicillin, streptomycin and fungizone B. The obtained culture medium has strong stability and long shelf life of about 12 months, completely meet the demands of large-range transportation of the tissue engineered skin products, can effectively maintain the structure and functions of the tissue engineered skin in the transportation process, becomes solid after being cooled, and consequently has convenient carry in the transportation process.

Description

Technical field [0001] The invention belongs to a kind of biological reagents in tissue engineering skin research, especially a culture medium used for tissue engineering skin transportation. Background technique [0002] The skin is the largest organ of the human body. It is composed of three parts: the epidermis, the dermis and the subcutaneous layer. The epidermal layer is located in the outermost layer of the skin, with a thickness between 0.06 mm and 0.8 mm. Its microstructure is composed of a basal layer, a spinous cell layer, a granular cell layer and a stratum corneum. The stratum corneum is the final product of the differentiation of keratinocytes. In its composition, 75% to 80% is protein, 5% to 15% is lipid, and 10 to 15 layers of cells constitute a structure with a thickness of about 10 μm. The stratum corneum has many important physiological functions, which can effectively block the damage of external toxic compounds to active skin cells, and especially has an extre...

Claims

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Application Information

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IPC IPC(8): C12N5/08A61L27/60C12N5/071
Inventor 邹运动董益阳程艳王军兵
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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