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Embellisia oxytropis for synthesizing spherosin, preparation and use thereof

A technology of swainsonin and Oxytropis, applied in the field of microorganisms, can solve the problems of lack of swainsonin sources and high price

Inactive Publication Date: 2009-07-22
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Swainsonine has good prospects for developing new anti-tumor drugs, but the source of swainsonine is very scarce and expensive, which is far from meeting the needs of people's research and clinical treatment

Method used

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  • Embellisia oxytropis for synthesizing spherosin, preparation and use thereof
  • Embellisia oxytropis for synthesizing spherosin, preparation and use thereof
  • Embellisia oxytropis for synthesizing spherosin, preparation and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: Isolation and screening of fungus Ehrigia spinosa FEL1a-AVB3

[0029] 1) Wash the whole mutant Astragalus membranaceus grown on the Gobi in Minqin County, Gansu Province with deionized water to remove the dust on the surface of the mutant Astragalus membranaceus, use sterilized tweezers to separate the leaves, stems and flowers of the plant, and then separate the leaves, stems, The flowers were wrapped with gauze separately for surface disinfection.

[0030] 2) Use sterilized filter paper to absorb the water on the surface-sterilized plant tissue, then use sterilized scissors to cut leaves, stems, flowers, etc. Each tissue block was inoculated on the surface of water agar medium, and cultured in an incubator at 18°C.

[0031] 3) When the hyphae grow out from around the tissue block, pick the mycelium at the edge of the colony and inoculate it on the surface of the enrichment medium, and place it in an incubator at 18°C ​​for enrichment culture; collect th...

Embodiment 2

[0032] Embodiment 2: the identification of fungus Ehrigia spinosa FEL1a-AVB3

[0033] 1. Morphological characteristics

[0034] The colony of the FEL1a-AVB3 strain was round, white at first, and gradually changed to gray-green, gray-brown, and black with the extension of culture time, with a slight protrusion in the center ( figure 1 ). The mycelium has a transverse septum without a mediastinum, and some old mycelium give birth to conidiophores with few branches. Conidia and conidiophores with septum, without mediastinum, the septum of conidia is thicker, the number of septa ranges from 1 to 4, the spores are rod-shaped, nearly cylindrical, and the size of conidia is 20-80 μm ×6~12μm( figure 2 ).

[0035] 2) Characteristics of culture

[0036]The strain grows very slowly on PDA, PCA, Czapek and other media, and its growth rate is 0.53, 0.60 and 0.40mm / d, respectively. The optimum growth temperature is 18°C, and the growth is inhibited when the temperature is higher than ...

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PUM

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Abstract

The invention discloses an Oxytropis embellisia strain FEL1a-AVB3 that is used for swainsonine synthesis and preserved in China Center for Type Culture Collection with the preservation date of October 8th, 2008 and CCTCC NO. of M 208142. The invention further discloses a strain separation method. The FEL1a-AVB3 strain parasitizes inside tissues of astragalus variabilis including leaf, stem, flower and seed, accretes with the astragalus variabilis and does not cause diseases of the astragalus variabilis, in vitro cultivation discovers that the FEL1a-AVB3 strain can generate SW, participates in the SW biosynthesis of the astragalus variabilis and is closely related with toxicity of the astragalus variabilis, and consequently SW content in the astragalus variabilis can be lowered by inhibiting the FEL1a-AVB3 growth in the astragalus variabilis, and the goal for reducing the astragalus variabilis toxication of animals can be achieved.

Description

technical field [0001] The invention belongs to the technical field of microbes, and relates to a new type of Ehrigia spp. (Embellisaoxytropis), in particular to a type of Ehrysotropis capable of synthesizing the main toxin of madweed, swainsonine (Swainsonine, SW). FEL1a-AVB3 also relates to the preparation and application of the bacteria. Background technique [0002] Locoweed is the general term for the poisonous plants of the genus Oxytropis and Astragalus of the leguminous family, and it is also the most serious poisonous weed that endangers grassland animal husbandry in the world. In my country, locoweeds are widely distributed in the vast grasslands of Northwest, North and Southwest China, with an area of ​​damage reaching 11 million ha 2 , accounting for 2.8% of the country's grassland area. Variation Astragalus is a kind of locoweed Astragalus in my country, mainly distributed in Inner Mongolia, Xinjiang, Gansu, Qinghai, Ningxia and other places; Variation Astraga...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P17/18A61K31/437A61P35/00C12R1/645
Inventor 王建华余永涛王建娜赵清梅耿果霞
Owner NORTHWEST A & F UNIV
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