Method for extracting amber acid from fermentation liquor

Abstract

The invention discloses a method for extracting succinic acid from a broth. The method comprises the following steps: a sodium succinate broth generated from microbial fermentation goes through a microfiltration membrane to conduct cross-flow filtration so as to remove thallus and other relatively large impurity particles; cross-flow filtration is carried out through a nanofiltration membrane to remove residual protein and pigment in the broth so as to get a clean filtrate; after the electrolysis, electrodialysis and electrolysis of the clean filtrate through a bipolar membrane, a succinic acid solution and a sodium hydroxide solution are obtained respectively; and the succinic acid solution is decompress-vaporized, cooled and crystallized, and therefore white succinic acid crystals are obtained. All working procedures in the method are closely connected, feed liquid always recycles under sealed condition, conditions through the whole technique process are mild, no organic solvent and any other organic compounds are added, the technique flow is short, steps are simple, the requirements on equipment are not high, the final product has high purity and the content of succinic acid is more than 99.0 percent through test.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and relates to a method for extracting succinic acid from fermentation liquid. Background technique [0002] Succinic acid (succinic acid) is an important C4 platform compound, which is widely used in medicine, pesticides, dyes, fragrances, paints, food, plastics and photographic materials industries. It is the basic raw material of important bulk chemicals and specialty chemicals such as 1,4-butanediol, tetrahydrofuran, γ-butyrolactone, PBT resin, N-methylpyrrolidone, adipic acid, etc. [0003] The preparation methods of succinic acid mainly include chemical synthesis and microbial fermentation. Chemical methods mainly include paraffin oxidation, cyanidation hydrolysis of methyl chloroacetate, ozonation of cycloalkenes, catalytic hydrogenation of maleic anhydride or fumaric acid, and electrolysis of maleic acid. The production of succinic acid in the chemical synthesis method will...

AI Technical Summary

Problems solved by technology

It is reported that succinic acid is extracted by using a solvent, but a large amount of expensive organic solvents are consumed during the extraction process, resulting in high purification costs, and the toxicity of residual solvents has a negative impact on the quality of food-grade and pharmaceutical-grade products. The method is not suitable for the fermentation broth system with high ionic strength

[0009] Chinese patent CN1887843 discloses a method for extracting succinic acid by microfiltration membrane filtration, then ultrafiltration membrane filtration, activated carbon decolorization, concentration, and crystallization, but this method cannot convert succinate into succinate Acid, and finally add acid to acidify, which will lead to the introduction of a large amount of salt in the solution, resulting in difficulty in purifying the product and low yield

[0010] Chinese patents CN101348428A and CN101348429A disclose a method for purifying succinic acid with anion and cation resins. It consumes a large amount of water in the process of ion exchange, which makes the evaporation cost higher, increases the energy cost, and the regeneration process will produce a lot of waste water

At the same time, it is used for the high salt content in the fermentation liquid, resulting in serious resin pollution, difficult regeneration, and shortened resin life.

[0011] In the above-mentioned extraction method, there may be problems of high post-treatment purification cost, or the reagent materials used are relatively expensive, the process route is long, or the method is too complicated, and the problem of high energy consumption is not conducive to the large-scale production of succinic acid produced by fermentation