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Exfoliated cell preservative fluid

A technology for exfoliated cells and preservation solution, applied in the field of exfoliated cell preservation solution, can solve the problems of high cost, high price, cell variation, etc., and achieve the effect of ensuring health and safety, intact cell shape, and uniform cell distribution

Inactive Publication Date: 2009-07-22
GUANGZHOU HONGQI OPTICAL INSTR TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, if it is placed away from the original living environment, the physiological function, biological activity, and inherent cell shape and structure of the cells will change. The conventional method is to cryopreserve the exfoliated cells, and cryopreservation requires special cryopreservation. The equipment is relatively expensive, and, due to the danger of cell damage during the freezing and thawing process, it will cause cell variation or deformation, which will affect the follow-up work
[0003] Thus, a solution specially used for exfoliated cell preservation has appeared on the market, such as the preservation solution (PresetverCyt) produced by U.S. Tinpreper TCT. TM ), the preservation solution is a reagent used with the machine Tinpreper-2000, which is expensive

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: the exfoliated cell preservation solution in the present embodiment comprises as follows:

[0035] Components in percentage by weight: 0.7% sodium chloride, 0.25% trisodium phosphate, one of 7.5% edetate disodium, edetate dipotassium, heparin, 1% sodium benzoate (or potassium salt), 3% paraformaldehyde, 3% coumarin, 0.1% proteinase K, 0.3% sodium bicarbonate, 2.5% carbolic acid,.

[0036] Components in volume percent: 60% pH buffer (pH7), 16% absolute ethanol, 12.5% ​​methanol, 3% acetone, wherein the pH buffer is one of PBS, Tris HCl, and PIPES.

Embodiment 2

[0037] Embodiment 2: the exfoliated cell preservation solution in the present embodiment comprises as follows:

[0038] Components in percentage by weight: 0.9% sodium chloride, 0.1% trisodium phosphate, one of 10% edetate disodium, edetate dipotassium, heparin, 1% sodium benzoate (or potassium salt), 4% paraformaldehyde, 4% coumarin, 0.5% proteinase K, 0.5% sodium bicarbonate, 1% carbolic acid.

[0039] Components in volume percentage: 50% pH buffer (pH6.5), 20% absolute ethanol, 15% methanol, 4% acetone, wherein the pH buffer is one of PBS, Tris HCl, and PIPES.

Embodiment 3

[0040] Embodiment 3: the exfoliated cell preservation solution in the present embodiment comprises as follows:

[0041] Components in percentage by weight: 0.5% sodium chloride, 0.5% trisodium phosphate, one of 5% edetate disodium, edetate dipotassium, heparin, 1% sodium benzoate (or potassium salt), 1% paraformaldehyde, 4% coumarin, 0.3% proteinase K, 0.1% sodium bicarbonate, 5% carbolic acid.

[0042] Components in volume percent: 70% pH buffer (pH7.4), 12% absolute ethanol, 10% methanol, 2% acetone, wherein the pH buffer is one of PBS, Tris HCl, and PIPES.

[0043] The test results show that the exfoliated cell preservation solutions of Example 1, Example 2 and Example 3 have a high cell preservation rate and a long preservation time, and can be preserved for 2 years at room temperature.

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PUM

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Abstract

The invention relates to a preservation solution for exfoliated cells, which is characterized by comprising a pH buffering agent, an osmotic pressure maintaining agent, an anti-corrosion antistaling agent, a fixative for maintaining cellular morphologies, an anticoagulant, a mucus softener, proteinase K and a component for destroying red blood cells. Compared with the prior art, the preservation solution has the advantages that the cell storage rate is high, the storage time at normal temperature is long, the mucus is sufficiently dissolved and the red blood cells are partially destroyed, the sheet-making effect comprises quite even cell distribution, the cellular morphologies are good, the cytolymph and the cell nucleus are divided clearly and are well arranged, the brightness of the cytolymph and the cell nucleus is quite good, and the karyoplasms, the morphological structures and the amount of the cells are quite clear.

Description

technical field [0001] The invention relates to an exfoliated cell preservation solution. Background technique [0002] Living cells have physiological functions, biological activities, and inherent cell morphology and structure, and have application value in many aspects. However, if it is placed away from the original living environment, the physiological function, biological activity, and inherent cell shape and structure of the cells will change. The conventional method is to cryopreserve the exfoliated cells, and cryopreservation requires special cryopreservation. The equipment is relatively expensive, and because of the danger of cell damage during the freezing and thawing process, it will cause cell variation or deformation, which will affect the follow-up work. [0003] Thus, a solution specially used for exfoliated cell preservation has appeared on the market, such as the preservation solution (PresetverCyt) produced by U.S. Tinpreper TCT. TM ), the preservation s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 陈文武
Owner GUANGZHOU HONGQI OPTICAL INSTR TECH
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