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Preparation method of effective component of Hydrocotyle sibthorpioides and application

A technology of effective parts and coriander, applied in the field of extraction and application of traditional Chinese medicine, can solve the problems of no ideal drug, large toxic and side effects, etc.

Inactive Publication Date: 2009-07-08
张如松
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In recent years, although some progress has been made in the treatment of anti-hepatic fibrosis, there is still no ideal drug at present. More than a dozen drugs such as colchicine and penicillamine have the effect of inhibiting liver fibrosis, but their side effects are relatively large , limiting its clinical use

Method used

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  • Preparation method of effective component of Hydrocotyle sibthorpioides and application
  • Preparation method of effective component of Hydrocotyle sibthorpioides and application
  • Preparation method of effective component of Hydrocotyle sibthorpioides and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) The medicinal material of coriander is extracted 3 times with 8 times the weight of ethanol, the concentration of ethanol is 50%, and each time is 1 hour; the verification of the extraction process: take 2kg of the medicinal material, extract according to the best extraction process, and use the extraction method of HAS Obtaining rate is an investigation index, and the result is that the transfer rate of HAS is 87.2%, and the extraction rate of effective parts is 1.2% based on the total amount of HAS and HHAS;

[0019] (2) After the above-mentioned extract is decompressed and recovered ethanol, put it on the HPD400 type macroporous resin column, the concentration of the sample solution is 0.20mg (crude drug) / ml, and the ratio of the sample amount to the crude drug and resin is 1:4 (kg / L), eluted with 70% ethanol after 3 volumes of water elution;

[0020] (3) Take the purified effective part, use silica gel column chromatography, elute with CHCl3:MeOH:H20=20:3:1 (up...

Embodiment 2

[0022] (1) Coriander Coriander medicinal material is extracted 2 times with 6 times the weight of ethanol, the concentration of ethanol is 60%, each time for 1 hour;

[0023] (2) After the above-mentioned extract is decompressed and recovered ethanol, put it on the HPD400 type macroporous resin column, the concentration of the sample solution is 0.25mg (crude drug) / ml, and the ratio of the sample amount to the crude drug and resin is 1:4 (kg / L), eluted with 50% ethanol after 3 volumes of water elution;

[0024] (3) Take the purified effective part, use silica gel column chromatography, elute with CHCl3:MeOH:H2O=20:3:1 (upper layer)-CHCl3:MeOH:H2O=10:3:1 (upper layer), collect CHCl3:MeOH:H2O=10:3:1 eluate, recover the solvent under reduced pressure, and recrystallize multiple times with methanol to obtain HAS with a yield of 0.52% (HSS: crude drug).

Embodiment 3

[0026] (1) Coriander Coriander medicinal material is extracted once with 10 times the weight of ethanol, and the concentration of ethanol is 70%;

[0027] (2) Go up NKA-9, D101, AB-8 or HPD100 macroporous resin column after decompression recovery ethanol of above-mentioned extract solution, the concentration of sample solution is 0.25mg (crude drug) / ml, 0.30mg (crude drug) / ml ml, 0.15mg (crude drug) / ml sample amount equivalent to the ratio of crude drug to resin is 1:4 (kg / L), eluted with 3 volumes of water and then eluted with 60% ethanol;

[0028] (3) Take the purified effective part, use silica gel column chromatography, elute with CHCl3:MeOH:H2O=20:3:1 (upper layer)-CHCl3:MeOH:H2O=10:3:1 (upper layer), collect CHCl3:MeOH:H2O=10:3:1 eluate, recover the solvent under reduced pressure, and recrystallize multiple times with methanol to obtain HAS with a yield of 0.49% (HAS: crude drug).

[0029] In vitro Inhibition Test of One Effective Fraction on HepG2 Cells Transfected by ...

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Abstract

The invention relates to a method for preparing the valid part of hydrocotyle sibthorpioides and an application thereof, belonging to the technical field of traditional Chinese medicine extraction and application. The method for preparing the valid part of the hydrocotyle sibthorpioides includes the following steps: 1-10 times of weight of ethanol is added to the hydrocotyle sibthorpioides, the obtained mixture is extracted by 1 to 3 times, and the concentration of the ethanol ranges from 30 percent to 70 percent; after the ethanol in the extracted solution is processed by the operation of depressurization and recycling, the extracted solution is led to a macroporous resin column, and after being eluted by 3 volumes by water, the extracted solution is eluted by the ethanol with the concentration ranging from 50 percent to 70 percent; the gained purified solution is processed by the operation of silica column chromatography, the eluted solution is collected, a dissolvent is depressurized and recovered, and finally, multiple times of methanol recrystallization is carried out to obtain the valid part of the hydrocotyle sibthorpioides; and the valid part of the hydrocotyle sibthorpioides has the functions of treating and preventing the hepatofibrosis. The invention has the advantages of reasonable design, easy process and low cost, and the valid part of the hydrocotyle sibthorpioides is derived from natural medicines; compared with the intraclass chemical medicine treating and preventing the hepatofibrosis, the valid part of the hydrocotyle sibthorpioides has low toxicity and high efficiency. Proven by the exosomatic inhibition test of HBV transfected HepG2 cells and drug effect experiments relating to the hepatofibrosis prevention, the valid part has prominent pharmacological action.

Description

technical field [0001] The invention belongs to the technical field of extraction and application of traditional Chinese medicines, and in particular relates to a preparation method of the effective part of Coriander coriander and its application in the preparation of anti-hepatic fibrosis drugs. Background technique [0002] Hepatic fibrosis refers to the abnormal proliferation of fibrous tissue in the liver when liver cells undergo necrosis and inflammatory stimulation. important link. There are many pathogenic factors that cause liver cell necrosis and inflammation. In foreign countries, especially in Europe and the United States, alcoholism is the most common factor. In my country, it is mainly caused by hepatitis virus. [0003] Liver fibrosis caused by viral hepatitis is caused by chronic inflammation in the liver after the human body is infected by hepatitis virus, resulting in fibrous tissue hyperplasia. Current studies have shown that the viruses that cause chroni...

Claims

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Application Information

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IPC IPC(8): A61K36/23A61P1/16
Inventor 张如松
Owner 张如松
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