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Preparation method of sea-ear active peptide

An active peptide, abalone technology, applied in food preparation, animal protein processing, food science and other directions, can solve the problem of low added value of abalone processing, and achieve the effects of improving immunity, rich nutrition and easy operation.

Inactive Publication Date: 2009-06-17
SHANDONG HOMEY AQUATIC DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the patent 200510047409 discloses the extraction method of abalone polysaccharides, which involves the method of extracting polysaccharides from abalone viscera, and the leftovers of abalone processing - abalone viscera are discarded, resulting in the disadvantage of low added value of abalone processing. So far, it has not been seen A report on the production of abalone active peptides from abalone leftovers

Method used

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preparation example Construction

[0009] A method for preparing an abalone active peptide, which comprises dehydrating and drying abalone leftovers, grinding them into fine powders of 100-200 mesh, adding 10-30 times of pure water, heating to eliminate microorganisms, adding proteolytic enzymes after cooling to carry out enzymatic hydrolysis, After enzymolysis, heat to inactivate the enzyme, and after cooling, centrifuge to remove insoluble matter, add 95% ethanol to the supernatant to make the system concentration reach 50-60% ethanol concentration, centrifuge to remove the precipitate after resting, concentrate the supernatant under reduced pressure to remove ethanol, use Decolorize with activated carbon, and finally dry to obtain abalone active peptide.

[0010] The proteolytic enzyme of the present invention is compounded by one or more of alkaline protease, acid protease, neutral enzyme, flavor enzyme, compound enzyme and papain.

[0011] The specific process method is:

[0012] a, the preparation of aba...

Embodiment 1

[0021] A kind of preparation method of abalone active peptide, abalone leftovers are dehydrated and dried, beaten into 100-200 mesh fine powder, add 10-30 times of pure water, heat to kill microorganisms, add compound enzyme after cooling (enzyme dosage and abalone leftovers The ratio of feed powder is 1:100-500) for enzymolysis, after enzymolysis, heat to inactivate the enzyme, after cooling, centrifuge to remove insoluble matter, add 95% ethanol to the supernatant to make the system concentration reach 50-60% ethanol concentration, stand still Afterwards, the precipitate was removed by centrifugation, the supernatant was concentrated under reduced pressure to remove ethanol, decolorized by activated carbon, and finally dried to obtain the abalone active peptide. specifically is:

[0022] a, the preparation of abalone leftovers powder select abalone leftovers to dry, make its moisture finally 10%, utilize the universal pulverizer to beat into fine powder of 100-200 purpose th...

Embodiment 2

[0031] A kind of preparation method of abalone active peptide, concrete steps are:

[0032] a, the preparation of abalone leftovers powder select abalone leftovers to dry, make its moisture finally 8%, then utilize universal pulverizer to beat into 100-200 purpose fine powder;

[0033] b. Enzymatic hydrolysis Take 50kg of abalone offal powder, add its quality of 1000L of pure water, heat treatment, make the solution center temperature reach 95-100°C for 10min, cool to 50°C, adjust the pH of the solution to 2, add 0.5kg of acid Hydrolyze with protease at a constant temperature of 50°C for 2 hours, adjust the pH value to 10, add 0.5 kg of alkaline protease, hydrolyze at a constant temperature of 50°C for 2 hours, adjust the pH value to 7 after the hydrolysis is completed;

[0034] c. Enzyme inactivation Heat the above solution to inactivate the enzyme so that the central temperature of the solution reaches 95°C for 10 minutes;

[0035] d, centrifugation The protease hydrolyzate...

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Abstract

The present invention relates to a method of manufacturing abalone bioactive peptide, including: dewatering and drying the abalone offal, grinding into 100-200 mesh of fine powder, adding 10-30 times of pure water, cooling down and than adding the proteolytic enzyme for enzymolysis, heating for exterminating enzyme, cooling down and then centrifugation for removing the insolubles, adding 95% ethanol in the supernatant to make the system concentration achieves the 50-60% of ethanol concentration, centrifugation for removing precipitation after the supernatant is static, performing pressure reduction and concentration to the supernatant for removing ethanol, decolorizing by use of activated carbon, and finally drying to get the abalone bioactive peptide. The invention uses the abalone offal for preparing abalone bioactive peptide, and extracts the obtained abalone bioactive peptide, which has health-care effects such as eliminating fatigue and improving immunity. The manufacturing method has reasonable process, simple operation, easy realization for the industrial production, eutrophy processed products and health-care efficacy.

Description

technical field [0001] The invention relates to the technical field of extracting active substances, in particular to a process for preparing abalone active peptides from abalone leftovers. Background technique [0002] We know that abalone is one of the precious seafood. It is a high-protein, low-fat, low-cholesterol food and is known as the "crown of seafood". Abalone is rich in protein, as well as more nutrients such as calcium, iron, iodine and vitamin A. The meat of abalone also contains an ingredient called "baosu", which can destroy the necessary metabolites of cancer cells. In addition to being rich in nutrition, abalone has very fresh and tender meat, so it has been loved by people since ancient times. With the breakthrough of abalone farming technology, there are more and more enterprises processing abalone on a large scale. However, the leftovers in the abalone processing process - abalone viscera have not been fully utilized. According to the research, the visc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J3/04A23L1/333A23L1/28A23L33/10A23L33/18
Inventor 袁文鹏刘昌衡唐聚德夏雪奎孟秀梅张绵松王小军孙永军胡炜
Owner SHANDONG HOMEY AQUATIC DEV
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