Zinc and copper ion fluorescence probe and preparation method and application thereof
A technology of fluorescent probes and copper ions, which is applied in the field of chemical analysis and detection, can solve the problems of complex preparation of copper ion fluorescent probes, inability to simultaneously measure copper ions, complex reaction conditions, etc., and achieve good prospects for popularization and application, low cost, Significant effects
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Embodiment 1
[0028] Example 1 Preparation of zinc and copper ion fluorescent probe 1-(7-acetamide-1,8-naphthyridine)-2-(6-acetamide-pyridine)-ethylene compound
[0029] 2-Amino-3-formyl-pyridine, 2-amino-7-methyl-1,8-naphthyridine and acetic anhydride were stirred and refluxed at 140°C for 24 hours under nitrogen protection. The mixture was then poured into ice water and stirred overnight. A yellow solid was obtained by filtration, washed with a large amount of water, and dried to obtain a crude product. After passing through a silica gel column using chloroform / ethanol (10:1) as a developing solvent, a light yellow sample was obtained with a yield of 80%.
[0030] Basic data of fluorescent probes:
[0031] mp 180-181°C.
[0032] 1 H NMR (CDCl 3 , 400MHz): δ(ppm) 8.59(m, 1H), 8.53(d, 1H, J=8.8Hz), 8.18(m, 3H), 7.67(d, 1H, J=16.2Hz), 7.56(d, 1H, J=8.3Hz), 7.48(m, 1H), 7.37(d, 1H, J=16.2Hz), 2.33(s, 9H).
[0033] 13 C NMR (CDCl 3 , 100MHz): δ(ppm) 25.0, 26.7, 115.1, 120.0, 120.1, 12...
Embodiment 2
[0036] Example 2 Application of Zinc and Copper Ion Fluorescent Probes
[0037] Dissolve the fluorescent probe in methanol to a concentration of 5.0 × 10 -5 M, After adding the corresponding metal ion solution, the change of fluorescence intensity was measured. figure 2 , image 3 , Figure 4 , Figure 5 , Figure 7 , Figure 8 , Figure 9 , Figure 10 , Figure 11 The excitation wavelength is 362nm. Spectral analysis shows that the fluorescent probe molecule 1 has a high selectivity for zinc ions and copper ions, and the fluorescence of the low-concentration solution of the fluorescent probe molecule 1 is significantly enhanced after zinc ions are added and red shifted, and the color changes. It is not affected by potassium, calcium, sodium and magnesium ions, nor is it affected by solvents and complex anions of metal ions; fluorescence is quenched after adding copper ions, and it is not affected by solvents and complex anions of metal ions. Transient fluorescence ...
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