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Method for producing acetone-butanol by co-fermentation of pentoses and hexose

An acetone-butanol and co-fermentation technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., to achieve the effects of ensuring the safety of liquid fuels, improving innovation capabilities and R&D capabilities, and being conducive to environmental protection

Active Publication Date: 2011-06-15
GUANGZHOU INST OF ENERGY CONVERSION - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] There is no report on the co-fermentation of pentose and hexose to produce acetone butanol

Method used

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  • Method for producing acetone-butanol by co-fermentation of pentoses and hexose
  • Method for producing acetone-butanol by co-fermentation of pentoses and hexose
  • Method for producing acetone-butanol by co-fermentation of pentoses and hexose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: Add corn flour co-fermentation in xylose and glucose mixed sugar

[0029] The total sugar content is controlled to be 3w%, 5w%, 6w%, 7w%, 8w%, 9w%, and xylose accounts for 75w%, 60w%, 50w%, 42.8w%, 37.5w% and 33.3% of the total sugar respectively w%, the rest is glucose. Wherein corn flour accounts for 5w%, 20w%, 33w%, 42w%, 50w% and 55w% of total sugar respectively.

[0030] Add 1000ml of water to corn flour, gelatinize at 100°C for 1 hour, add xylose and glucose in proportion, mix well, and divide into 250ml Erlenmeyer flasks, each with 150ml. Sterilize at 121°C for 120 minutes in a sterilizer.

[0031] After sterilization, the culture medium was taken out and cooled to room temperature, and ATCC 51743 and CICC8012 strains were inserted into the culture medium with an inoculum size of 5%, and fermented in an anaerobic incubator at 35°C for 72 hours.

[0032] After the fermentation is complete, get 1ml of fermented liquid and place it in a centrifuge t...

Embodiment 2

[0035] Embodiment 2: Co-fermentation of adding organic additives in xylose and glucose mixed sugar

[0036] Take 35g of xylose, 35g of glucose and add 1000ml of water to form a sugar content of 7% by weight, organic additives (mixed preparation of various agricultural and sideline products, rich in phosphorus and nitrogen, including bran, rice bran, bean cake powder and bean cake, etc.) Respectively 15w%, 20w%, 25w%, 30w% and 40w% of the amount of total sugar, mixed evenly, and distributed to 250ml Erlenmeyer flasks, each Erlenmeyer flask is filled with 150ml. Sterilize at 121°C for 120 minutes in a sterilizer.

[0037] After sterilization, the culture medium was taken out and cooled to room temperature, and ATCC 51743 and CICC8012 strains were inserted into the culture medium with an inoculum size of 5%, and fermented in an anaerobic incubator at 35°C for 72 hours.

[0038]After the fermentation is complete, get 1ml of fermented liquid and place it in a centrifuge tube, cent...

Embodiment 3

[0041] Example 3: Adding corn flour and organic additives to co-fermentation in xylose and glucose mixed sugar

[0042] Medium 1: Take 3g of corn flour and add 1000ml of water, gelatinize the corn at 100°C for 1 hour, add 30g of xylose to control the total sugar content to 7%, the rest is glucose, and 0.5g of organic additives, of which corn flour and organic additives are 5% of the total sugar, mix well, put into 250ml Erlenmeyer flask, each Erlenmeyer flask is filled with 150ml, and sterilized at 121℃ for 120 minutes in a sterilizing pot;

[0043] Medium 2: Take 15g of corn flour and add 1000ml of water, add 30g of xylose after corn gelatinization at 100°C for 1 hour, control the total sugar content to 7%, and the rest are glucose and 7g of organic additives, of which corn flour and organic additives are the total 31.4% of sugar, mix evenly, dispense into 250ml Erlenmeyer flasks, each Erlenmeyer flask is filled with 150ml, and sterilize in a sterilizing pot at 121°C for 120 ...

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Abstract

The invention provides a method for producing acetone butanol by jointly fermenting pentose and hexose. The method comprises the following steps: the pentose and the hexose are mixed, the weight of the pentose against the total weight of the mixture is between 5 and 75 percent, the mixture is added with a nitrogen source or a phosphorus source to obtain a raw material, and the raw material is subjected to cooking sterilization to obtain cooking mash; the cooking mash is cooled to a temperature of below 42 DEG C and injected with strains and fermented; and the fermented solution is filtered, distilled and rectified to obtain final acetone, butanol and ethanol products. By the invention, the method which effectively utilizes the joint fermentation of the pentose and the hexose to produce the acetone butanol is obtained. According to local conditions, lignocellulose is utilized to hydrolyze pentose and the hexose for joint fermentation to produce the acetone butanol, thereby realizing waste reutilization, facilitating environmental protection, promoting diversification of energy structure in China, ensuring safety of liquid fuel in China and having positive significance on improving creativity and research capability in the field of preparing biomass liquid fuel in China.

Description

technical field [0001] The invention relates to a method for producing acetone-butanol by fermentation, in particular to a method for co-fermenting pentose and hexose to produce acetone-butanol. technical background [0002] Acetone butanol is an important organic solvent and chemical raw material, widely used in spray paint, explosives, plastics, pharmaceuticals, plant extraction, organic glass, synthetic rubber and other industries. What's more: Biobutanol, similar to ethanol, is a bioprocessed alcohol fuel and a new generation of liquid fuel. The main composition of gasoline is C6-C8, so butanol is more similar to "oil" than ethanol, and it has better compatibility with fuel additives and lubricating oils. Butanol's chemical structure gives it several advantages over ethanol, including low vapor pressure and less water pollution when mixed with petroleum, allowing it to be transported using existing petroleum transportation pipelines. Ethanol readily absorbs water molec...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12R1/145C12P7/36C12P7/08C12P7/16
Inventor 陈新德袁浩然彭万峰危巍陈勇
Owner GUANGZHOU INST OF ENERGY CONVERSION - CHINESE ACAD OF SCI
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