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Human immunodeficiency virus antigen/antibody chemiluminescence immune assay determination kit and method for preparing same

A technology for human immunodeficiency and virus antigens, applied in the field of immunoassay medicine, can solve the problems of need, limited popularization, and inability to be widely used in clinical diagnosis and scientific research, and achieves improved sensitivity, wide application range, and shortened detection window period. Effect

Inactive Publication Date: 2009-02-11
CHEMCLIN DIAGNOSTICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the chemiluminescence immunoassay kit in the prior art is a closed fully automatic chemiluminescence measurement system, which requires an expensive fully automatic chemiluminescence measuring instrument, thus limiting its popularization and use, and cannot be widely used in clinical diagnosis and scientific research.

Method used

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  • Human immunodeficiency virus antigen/antibody chemiluminescence immune assay determination kit and method for preparing same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1 Preparation of Human Immunodeficiency Virus Antigen / Antibody Chemiluminescence Immunoassay Assay Kit of the present invention

[0061] The human immunodeficiency virus antigen / antibody chemiluminescent immunoassay detection kit of the present invention contains the following components: a pre-coated luminescent plate coated with HIV antigen and P24 monoclonal antibody at the same time; an enzyme label labeled with horseradish peroxidase HIV antigen marker and enzyme-labeled streptavidin; biotinylated P24 antibody; washing solution containing Tween20 phosphate buffer (20 times concentrated washing solution); containing luminol, Tween20 and its enhancer benzofluor Chemiluminescence substrate solution A of anthracene; chemiluminescence substrate solution B containing hydrogen peroxide; negative control of normal human serum; HIV antibody positive control of HIV antibody-positive human serum diluted in neonatal bovine serum; and diluted P24 antigen in neonatal bovi...

Embodiment 2～3

[0076] Embodiment 2~3 preparation human immunodeficiency virus antigen / antibody chemiluminescence immunoassay assay kit of the present invention

[0077] The kit of the present invention was prepared in the same manner as in Example 1 except that plastic beads and magnetic particles were used as carriers respectively.

Embodiment 4

[0078] Embodiment 4 The using method of kit of the present invention

[0079] 1. Add samples, biotinylated antibodies and controls to the chemiluminescent coated plate

[0080] Take the HIV antigen antibody coated plate, equilibrate to room temperature, set 3 wells for negative control, 2 wells for HIV antibody positive control, and 2 wells for HIV-1P24 antigen positive control for each experiment, add 100 μl to each well, and add 100 μl to each well of the sample well Add 50 μl of the sample, then add 50 μl of biotinylated P24 antibody, mix well, stick the plate sealing film, and incubate at 37°C for 30 minutes.

[0081] 2. Wash the plate with washing solution

[0082] Shake off the reaction solution, wash the plate 5 times with diluted washing solution, and finally dry it on clean absorbent paper.

[0083] 3. Add enzyme markers

[0084] In addition to the blank wells, add 100 μl of horseradish peroxidase-labeled HIV1+2 antigen and horseradish peroxidase-labeled streptavid...

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Abstract

The invention relates to the immunization analysis medical field, and particularly provides a kit for determining the human immunodeficiency virus (HIV) antigen / antibody by the chemoluminescent immunization analysis and a preparation method thereof. The kit comprises (1) a solid carrier coated by the HIV antigens and antibodies; (2) biotin-labeled HIV antibodies; (3) enzyme-labeled HIV antigens and streptavidin; (4) a chemoluminescent primer acting with the above enzyme; and (5) a contrast. The preparation method of the kit includes the following steps of (1) coating the solid carrier with the HIV antigens and antibodies; (2) labeling the HIV antigens with a biotin; (3) labeling the HIV antigens and the streptavidin with the enzyme; (4) preparing the chemoluminescent primer, (5) preparing the contrast; (6) separately packaging; and (7) assembling into a finished product. The kit has the advantages of simpliness, rapidness, sensitiveness, stability and the like.

Description

technical field [0001] The invention relates to the field of immunoanalysis medicine, in particular, the invention provides a human immunodeficiency virus antigen / antibody assay kit and a preparation method thereof. Background technique [0002] AIDS, or Acquired Immure Deficiency Syndrome (AIDS), is caused by human immunodeficiency virus (human immunodeficiency virus, HIV). According to the different clinical manifestations of AIDS, it can be divided into four periods: acute infection period, incubation period, pre-AIDS period, and typical AIDS period. AIDS in the acute infection stage usually only manifests as mild symptoms such as fever, rash, enlarged lymph nodes, fatigue, sweating, nausea, vomiting, diarrhea, and pharyngitis. HIV antibodies can appear, which can be used as an important indicator for early diagnosis and disease screening. After the symptoms of the acute phase last for 3 to 14 days, AIDS has an asymptomatic incubation period of varying lengths (average ...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/543G01N21/76
Inventor 詹先发应希堂胡国茂
Owner CHEMCLIN DIAGNOSTICS CO LTD
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