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Method for preparing collagen protein

A collagen and raw material pretreatment technology, applied in the field of medical grade collagen and sponge, can solve the problems of physiological activity destruction, affecting collagen physiological activity, limiting application range, etc., and achieve strong tissue affinity and strong tissue repair function , good hemostatic performance

Inactive Publication Date: 2009-02-11
无锡贝迪生物工程股份有限公司
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Gelatin (one of the components of jelly) or some commercially available hydrolyzed collagen is almost the same as protein hydrolyzate (amino acid), its original physiological activity has been destroyed, and different treatment methods will affect the original collagen. Physiological activity, but if the collagen molecule is not further processed according to the purpose of development, its application range will be limited

Method used

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  • Method for preparing collagen protein
  • Method for preparing collagen protein
  • Method for preparing collagen protein

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Embodiment Construction

[0012] Mainly pretreatment→acidification treatment with acetic acid→extraction (sodium chloride differential precipitation)→purification (filtration, chromatography)→concentration (ultrafiltration, ion exchange resin, Akta, freeze-drying)→cutting→double packaging→sterilization ( гline) → finished product.

[0013] 1) Pre-treatment: After removing the fat and fascia of the fresh beef tendon, take it out after freezing and hardening at 4°C, scrape off the residual fascia on the surface of the beef tendon with a scalpel, and cut it into thin slices of appropriate size along the direction of the tendon fibers. Weigh, soak in 0.1% sodium carbonate (CP) solution for 4 hours, rinse with distilled water several times, filter the aqueous solution with a stainless steel mesh, and dry it for later use.

[0014] 2) Extraction of collagen: put the above-mentioned pretreated beef tendon into an Erlenmeyer flask, add an appropriate amount of 0.3% acetic acid (CP) solution (pH=2-3) containing...

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Abstract

A method for preparing collagen comprises the steps: 1) preliminary treatment of materials: body fat and aponeurosis of fresh beef tendon are removed, and then the beef tendon is soaked in 0.1 plus or minus 0.05% of sodium carbonate solution for 4 plus or minus 2h and is rinsed by distilled water for a plurality of times for airing; 2) pretreated beef tendon is added with proteolytic enzyme according to the mass mixture ratio of 0.3 plus or minus 0.1wt% and is added with acetic acid solution to be slowly stirred for 3-5d at the temperature of 1-12 DEG C; and then, the mixture is centrifugated by a high speed freezing centrifuge, and supernatant fluid is taken out and extracted crudely to obtain collagen solution; 3) the purification of the collagen: the crude extracted collagen solution is added with H2O2 solution with the mass percentage of 1 plus or minus 0.5% to be evenly mixed in a rest state for 4 plus or minus 2h, and the pH value is adjusted by 5 plus or minus 1 by trisodium citrate solution; after centrifugation, certain quantity of sodium chloride is added into the residual solution for salting out, and then the precipitate is filled into a bag filter to be dialyzed by acetic acid solution for 1 plus or minus 0.5d and by distilled water for 3 plus or minus 1d; dialysate is changed for 2-3 times every day, and collagen liquid is obtained.

Description

1. Technical field [0001] The invention relates to medical-grade collagen and a sponge, and relates to a high-purity and complete triple-helix macromolecular active collagen. Especially the preparation of biochemical and pharmaceutical grade high-purity collagen, especially the process for obtaining soluble medical grade collagen from animal connective tissue and medical products including soluble collagen produced by this process. It can be widely used in biomedicine, biomaterial preparation, beauty care and other industries. 2. Background technology [0002] Collagen is a structural protein that makes up various extracellular matrices and plays a role of binding tissue in animal cells. It is the protein with the most content in animals, accounting for about 25-35% of human protein, equivalent to 6% of human body weight, and is distributed throughout various tissues and organs throughout the body, such as bones, cartilage, ligaments, skin, cornea, various endometrium, Fas...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/14
Inventor 任伟业李峰杨庆
Owner 无锡贝迪生物工程股份有限公司
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