B cell epitope of epididymal protease inhibitor and multiple antigenic peptide containing thereof

Abstract

The invention discloses an epididymis protease inhibition protein B-cell epitope and a multiple antigenic peptide containing the epitope; the epididymis protease inhibition protein B-cell epitope of the invention has amino acid sequences shown in SEQ ID No.1 and has single component and simple structure, and is free of the impact of bad epitopes in natural proteantigens, and immunoreactions initiated by the epitope of the invention has strong pertinence; the multiple antigenic peptide containing the epididymis protease inhibition protein B-cell epitope, is obtained by connecting a T accessory cell epitope with the epididymis protease inhibition protein B-cell epitope on a branch of a tree-based core formed by a bifunctional unit or a plurality of bifunctional units; the multiple antigenic peptide can induce that an antibody of the epididymis protease inhibition protein B-cell epitope is targeted with high titer in a specific manner and a reversible anti-fertility function with high efficiency and safety is produced, thereby being capable of preparing a male pregnancy vaccine, having significant social and economic benefits and having important value in theory and wide application prospect.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to epididymis protease inhibitory protein B cell epitope and multiple antigenic peptides containing the epitope. Background technique [0002] Family planning is the basic national policy of our country. In order to effectively control the excessive population growth, the development of efficient, safe and reversible contraceptive methods has always been an important topic of family planning research. At present, male contraception is still limited to the traditional use of condoms or vasectomy, the former has a high failure rate, and the latter is irreversible and may have unexpected and serious immunological consequences, such as persistently high levels of antisperm antibody drops Degree and / or changes in testicular morphology, both of which are unsatisfactory. Over the years, scientists at home and abroad have been working on the development of male contraceptive vaccines, mainly t...

AI Technical Summary

Problems solved by technology

At present, male contraception is still limited to the traditional use of condoms or vasectomy, the former has a high failure rate, and the latter is irreversible and may have unexpected and serious immunological consequences, such as persistently high levels of antisperm antibody drops degree and / or changes in testicular morphology, neither of which is satisfactory

Over the years, scientists at home and abroad have been committed to the development of male contraceptive vaccines, mainly targeting reproductive hormones and sperm-specific proteins, such as sperm membrane antigen PH-20, fertilization antigen FA-1, acrosome antigen SP-10 , fertilin β, etc. The multivalent vaccines that have been developed can significantly inhibit the combination and fusion of sperm and eggs in animals, but the contraceptive rate still remains at about 50%.

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