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Test paper strip for detecting one or more porcine virus diarrhea disease antibody

A test strip and virus technology, which is applied in the field of test strips for detecting one or more swine viral diarrhea disease antibodies, can solve the problems that are not suitable for rapid detection or diagnosis, the operation is not simple and fast enough, and there are multiple operation steps and experience. problem, to achieve the effect of not easy false negative and false positive misjudgment, intuitive display, and intuitive result judgment

Inactive Publication Date: 2009-02-04
HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Detecting antibodies by cultivating viruses requires laboratory conditions, senior professionals, and relatively expensive instruments and equipment. Detects antibodies against a swine disease
It can be seen that although the above methods are specific or sensitive in detection, they are not easy and fast enough to operate, and are not suitable for rapid detection or diagnosis at the grassroots level or on-site.

Method used

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  • Test paper strip for detecting one or more porcine virus diarrhea disease antibody
  • Test paper strip for detecting one or more porcine virus diarrhea disease antibody
  • Test paper strip for detecting one or more porcine virus diarrhea disease antibody

Examples

Experimental program
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Effect test

preparation example Construction

[0026] 1. Preparation of goat or rabbit anti-SPA protein IgG

[0027] Inject negative healthy sheep or rabbits 3-4 times subcutaneously and intramuscularly with 50μg~100μg SPA / kg body weight. 20 days after the last immunization, blood is collected from the vein, and the serum antibody titer is above 1:2000 as determined by ELISA. Blood collection from the heart or neck Arterial bloodletting and collection of hyperimmune serum. Take 1 part of serum and 2 parts of PBS solution (pH7.2) and mix well, add an equal volume of saturated ammonium sulfate solution and mix well, put it in a refrigerator at 4°C for 2 hours, centrifuge at 4°C and 10000r / min for 15min, discard the supernatant; Appropriate amount of PBS solution (pH7.2) to dissolve the precipitate, add saturated ammonium sulfate solution to the final concentration of 33%, put it in the refrigerator at 4°C for 2h, centrifuge at 4°C and 10000r / min for 15min, discard the supernatant, and use a small amount of PBS solution (pH7...

Embodiment 1

[0042] Embodiment one: see Figure 1 ~ Figure 2 , in the figure, 1 is the support layer, made of hard plastic strips, 2 is the test end fiber layer, made of glass wool, 3 is the fiber layer adsorbed with gold standard SPA (prepared according to the above-mentioned specific preparation method 4) Gold standard SPA glass wool), 4 is the cellulose membrane layer, using nitrocellulose membrane, 5 is the water-absorbing material layer, made of water-absorbing filter paper, and the layers numbered 2, 3, 4, and 5 are pasted on the On the plastic sheet strip 1 , the fibers at the junction between each other intersect and infiltrate each other. On the nitrocellulose membrane layer 4, use the purified TGEV, PDEV and RV virus solutions to print the detection blots T, P, R respectively, and use the goat or rabbit anti-SPAIgG solution to print the control blots C, blots T, P, R, C is arranged in the form of Any one of them, 8-1 is the test end white plastic protective film ...

Embodiment 2

[0043] Embodiment two: see Figure 1 ~ Figure 2 , the present embodiment is basically the same as Example 1, and the similarities will not be repeated. The difference is that: 3 is the fiber layer adsorbing the gold-labeled antigen, which is the fiber layer adsorbed with gold-labeled purified TGEV, PDEV and RV virus Gold-labeled antigen glass wool prepared according to the above-mentioned specific preparation method 4), on the nitrocellulose membrane layer 4, C is combined and printed with the IgG solution against TGEV, PDEV and RV Shape control blot.

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Abstract

The present invention relates to a test strip which is used for testing a kind of or a plurality of kinds of disease antibodies of porcine virus diarrhea; the test strip comprises a supporting layer and a reaction reagent carrier absorbing layer which is pasted on the supporting layer; the reaction reagent carrier absorbing layer comprises a testing end fiber layer, a fiber layer of absorbing gold-labeled SPA protein or gold-labeled antigen which corresponds to the antigen to be tested, and a cellulose layer, which are arranged at the sample end in sequence, and an absorbent material layer which is positioned at the handle end; the cellulose layer contains one, two or three testing print(s) which are printed by anyone, any two or three of the purified transmissible gastroenteritis virus TGEV solution, the porcine epidemic diarrhea virus PDEV solution and the porcine rotavirus RV, and the cellulose layer also contains the contrast prints which are printed by anyone, any two or three of the anti-SPA protein IgG solution of the sheep or the rabbit or the anti-TGEV, anti-PDEV, anti-RV IgG solution of the sheep or the rabbit; and the invention provides a test strip for testing a kind of or a plurality of kinds of disease antibodies of the porcine virus diarrhea, which has the advantages of accurate and rapid detection, convenient operation and low costs.

Description

(1) Technical field: [0001] The invention relates to a device for detecting antibodies to porcine viral infectious diseases, in particular to a test strip for detecting one or more antibodies to porcine viral diarrhea diseases. (two), background technology: [0002] Porcine diarrheal disease is a kind of disease that seriously affects the development of pig industry, and viral diarrhea is especially difficult to control. Porcine epidemic diarrhea virus (PEDV, code P), porcine transmissible gastroenteritis virus (TGEV, code T) and rotavirus (RV, code R) are the main viruses that cause viral diarrhea in pigs. The clinical symptoms of porcine diarrheal diseases are similar, and there are often mixed infections in the same pig farm. Therefore, in order to prevent and treat porcine viral diarrhea in time, a specific method for rapid differential diagnosis of porcine viral diarrhea is needed, that is, rapid detection of related viruses or specific antibodies thereof is required. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/545G01N33/544
Inventor 张改平肖治军杨艳艳邓瑞广李学伍杨继飞赵东王爱萍邢广旭柴书军刘庆堂
Owner HENAN ACAD OF AGRI SCI
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