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Production method of haemachrome and decolorizing blood plasma albumen powder

A technology of hemoglobin powder and hemoglobin, which is applied in the fields of food chemistry and biochemistry, can solve the problems of high environmental damage, high cost, inconvenient operation, etc., and achieves the effects of easy reaction operation, improved thermal stability, and simple operation.

Inactive Publication Date: 2009-01-28
抚顺丹阳生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Organic solvent extraction can extract heme very well, but it needs a very large amount of organic solvent. Extracting 1kg of heme requires 800L-1000L of organic solvent, so the efficiency is low, which brings great inconvenience to the operation and is harmful to the environment. also bigger
U.S. Patent No. 4,376,727 proposes to use carboxymethyl cellulose to adsorb heme. This method can solve the separation of heme and globin, but what is obtained is a mixture of heme and carboxymethyl cellulose, and pure heme cannot be obtained, and carboxymethyl Base cellulose cannot be regenerated, so the cost of this method is relatively high, limiting its use
Chinese patents ZL87101834 and ZL200610080022.9 use surfactants as catalysts to crystallize heme, and the crystallization effect is better, but this method is prone to co-precipitation of globin and heme during the heating process. To solve this problem, it is necessary to accurately control the reaction process And use complex operating equipment; more importantly, the protein powder produced by this method contains surfactants, and its safety for humans and animals has been questioned

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Collect 300L of fresh pig blood from the slaughterhouse, add 1kg of sodium citrate for anticoagulation, add 300L of water and stir evenly, add 1kg of animal proteolytic enzyme, 1kg of flavor enzyme, adjust the pH value to 7.5, heat to 55°C, and enzymatically hydrolyze for 48 hours. Heat the enzymolysis solution to 90°C for 20 minutes to inactivate protease. After cooling, adjust the pH of the enzymolysis solution to 6.0 with hydrochloric acid, then centrifuge to collect the supernatant and precipitate respectively. The supernatant is slightly yellow, adjust the pH to 7 with sodium hydroxide solution, concentrate in three effects, and spray dry to obtain 50kg of white protein powder; add 50L of water to the precipitate (about 10kg of dry matter) and stir evenly, add 1L to make the mass concentration 38% Concentrated hydrochloric acid, 50kg Tween 20, continue to stir, then heat to 70°C to stop stirring, keep warm for 30min, centrifuge to collect the precipitate after cool...

Embodiment 2

[0021] The slaughterhouse collected 300L of fresh bovine blood, added potassium oxalate for anticoagulation, and separated by a continuous centrifuge to obtain 170L of plasma and 130L of blood cells. The blood plasma was spray-dried to obtain 13 kg of plasma protein powder. Add 200L water to the blood cells, stir for 30 minutes to hemolyze, adjust the pH value to 9.0 with 1M NaOH solution, add 4kg animal proteolytic enzyme, heat to 65°C, and enzymolyze until the degree of hydrolysis reaches 40%. After the enzymolysis is completed, the enzymolysis solution is boiled for 30 minutes, and after cooling, the pH is adjusted to 4.5 with hydrochloric acid and then press-filtered to obtain 280 kg of filtrate and 50 kg of filter residue (about 8 kg of dry matter). The filtrate was adjusted to pH 6.5 with sodium hydroxide solution, concentrated by ultrafiltration, and spray-dried to obtain 40 kg of protein powder. Add 1600L of water to the filter residue and stir evenly, add 80L of conc...

Embodiment 3

[0023] Take 500kg of chicken and duck blood from the slaughterhouse, coagulate into blood clots, crush it with a pulverizer, add 1000L of water, stir well, adjust the pH value to 7.0, add 60g of animal proteolytic enzyme, heat to 70°C, enzymolysis for 12 hours, the degree of hydrolysis was 5.6%. After adjusting the pH to 2.5 with hydrochloric acid, the supernatant and the precipitate were collected by centrifugation, and the supernatant was adjusted to pH 5.5 with sodium hydroxide solution, concentrated in three effects, and spray-dried to obtain 105 kg of white protein powder; Concentrated hydrochloric acid with a concentration of 36%, 4kg Tween 20, and 1kg Span 60, stirred evenly, heated to 75°C, stopped stirring, kept the heat for 15 minutes, cooled, centrifuged to collect the precipitate, washed with water, and dried to obtain the finished heme 2.7 kg, with a purity of 90%.

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Abstract

The invention discloses a manufacture method of hemachrome and discolored globin powder. The method comprises the steps that: after zymohydrolysis and equal point deposition of the raw material hemoglobin are carried out, the globin powder is prepared by supernatant by concentration and drying; and the hemachrome is prepared by precipitation and crystallization. The method of the invention can fully utilize the precious protein resources of poultry blood, jointly extract the hemachrome and eatable discolored globin powder and has not waste; the obtained discolored globin powder has good color, few impurities and high content of proteins. The method has the advantages of simple operation, low lost and clean without contamination, and being suitable for industrial production.

Description

technical field [0001] The invention relates to a production method of heme and decolorized blood globulin powder, belonging to the fields of food chemistry and biochemistry. Background technique [0002] Blood accounts for 7%-8% of the total protein of poultry and livestock. It is rich in nutrients and has a protein content of about 20%. It is called "liquid meat". Proteins in blood include albumin and hemoglobin, among which hemoglobin accounts for 70%-80%. However, due to the disgusting fishy smell of hemoglobin, except for a small amount used in blood tofu, most of them are discarded, which not only wastes a large amount of precious protein resources but also pollutes the ecological environment. Hemoglobin is a homotetramer composed of four subunits, each containing a globin and a heme bound to it. Among them, heme is an excellent blood enriching agent and an important pharmaceutical and chemical raw material. Therefore, making full use of blood resources can not only...

Claims

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Application Information

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IPC IPC(8): C12P21/06
Inventor 龚兴国郦剑勇秦勇曾冬云
Owner 抚顺丹阳生物科技股份有限公司
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