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Hyaluronic acid modified polycaprolactone/polylactic acid three-dimensional stephanoporate compound stent and preparation

A technology of polycaprolactone and hyaluronic acid, which is applied in the direction of conjugated synthetic polymer rayon, fiber processing, filament/thread forming, etc., can solve the problems such as no similar or similar patent reports.

Inactive Publication Date: 2009-01-28
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no patent report in China to use PCL, PLA and hyaluronic acid (HA) blends to prepare three-dimensional porous scaffold materials for tissue engineering
However, using PCL, PLA and HA as raw materials, there are no similar or similar patent reports at home and abroad for the method of preparing tissue engineering scaffold materials through electrospinning technology and plasma modification.

Method used

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  • Hyaluronic acid modified polycaprolactone/polylactic acid three-dimensional stephanoporate compound stent and preparation
  • Hyaluronic acid modified polycaprolactone/polylactic acid three-dimensional stephanoporate compound stent and preparation

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Using chloroform as a co-solvent, dissolve polymer PCL with a molecular weight of 50,000 and a melting point of 58-60°C, and polymer PLA with an average molecular weight of 100,000 and a melting point of 150-170°C in a ratio of 60:40 In a co-solvent, stir evenly to make a transparent and uniform solution, then electrospin to make an electrospun fiber mat, dry it in a vacuum oven, remove the residual solvent, take it out, stack it and fill it in a mold with a specific shape. The temperature of the vacuum oven was set at 63°C, and the mold was placed in the vacuum oven at a constant temperature for 20 minutes before being taken out, cooled and demoulded, then subjected to plasma modification, then soaked in hyaluronic acid solution, and then freeze-dried to obtain Scaffold material for tissue engineering with specific shape and good performance. The fiber mat fiber diameter measured by scanning electron microscope and Photoshop software is 2000±300nm, and the contact angl...

Embodiment 2

[0033]Using a mixed solvent of chloroform and DMF as a co-solvent, the high polymer PCL with a molecular weight of 100,000 and a melting point of 58-60°C, and the high-molecular PLA with an average molecular weight of 10,000 and a melting point of 150-170°C were mixed in a ratio of 40:60. Dissolve the ratio in the co-solvent, stir evenly, make a transparent solution, electrospin to make electrospun fiber felt, put it in a vacuum oven to dry, take it out, stack it and fill it in a mold with a specific shape, and put it in a vacuum oven The temperature was set at 63°C, and the mold was placed in a vacuum oven at a constant temperature for 35 minutes before being taken out, cooled and demolded, and then subjected to plasma modification, then soaked in hyaluronic acid solution, and then freeze-dried to obtain a specific Scaffold material for tissue engineering with good shape and good performance. The fiber mat fiber diameter measured by scanning electron microscope and Photoshop ...

Embodiment 3

[0035] Using a mixed solvent of tetrahydrofuran and acetone as a co-solvent, the polymer PCL with a molecular weight of 100,000 and a melting point of 58-60°C and the polymer PLA with a molecular weight of 10,000 and a melting point of 150-170°C are mixed in a ratio of 80:20 Dissolve in a co-solvent, stir evenly to make a transparent solution, electrospin to make an electrospun fiber mat, dry it in a vacuum oven, remove the residual solvent, take it out, stack it and fill it in a mold with a specific shape. The temperature of the vacuum oven is set at 60°C, the mold is placed in the vacuum oven at a constant temperature for 40 minutes, and then taken out, cooled and demoulded, then subjected to plasma modification, then soaked in hyaluronic acid solution, and then freeze-dried to obtain Scaffold material for tissue engineering with specific shape and good performance. The fiber mat fiber diameter measured by scanning electron microscope and Photoshop software is 3500±200nm, an...

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Abstract

The invention relates to a hyaluronic acid modified polycaprolactone / polylactic acid three-dimensional porous composite scaffold and a preparation method thereof. The composite scaffold comprises the components of polycaprolactone PCL, polylactic acid PLA and hyaluronic acid HA. The preparation method comprises: (1) dissolving PCL and PLA into a cosolvent to derive transparent blend solution; (2) adjusting electrostatic spinning process, spinning the blend solution to a nanometer / submicron graded fibrofelt, and vacuumizing in a vacuum oven to remove residual solvents; (3) overlapping and filling the fibrofelt to a die of specific shape and placing in the vacuum oven, cooling, demoulding, modifying a plasma, and immersing in hyaluronic acid HA solution; (4) placing the HA modified composite scaffold in the vacuum oven to be dried, sterilizing with epoxy ethane and deriving the three-dimensional porous composite scaffold. The three-dimensional porous tissue engineering composite scaffold material of the invention has the advantages of uniform internal pore structure, stable architecture and simple preparation method, and can be used as the scaffold of bone and cartilage tissue engineering cell.

Description

technical field [0001] The invention belongs to the field of biological tissue engineering carrier materials and preparation, in particular to a hyaluronic acid-modified polycaprolactone / polylactic acid three-dimensional porous composite scaffold and its preparation. Background technique [0002] The loss or dysfunction of tissues and organs seriously affects human health, leading to human disease and death. Although traditional autografts, allografts, and artificial substitutes have shown good therapeutic effects, their defects such as immune rejection, limited donor sources, or the need for secondary surgery limit their application, and the development of tissue engineering has made up for it. these defects. Tissue engineering collects function-related living cells, plants them on natural or synthetic three-dimensional scaffolds with a certain spatial structure, and cultures them in vitro. After the cells proliferate in large quantities, they are transplanted into the bod...

Claims

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Application Information

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IPC IPC(8): A61L27/40A61L27/20A61L27/18D01F8/14D01D5/00
Inventor 杨庆唐勇红郯志清沈新元
Owner DONGHUA UNIV
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