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Novel use of Cistanche tubulosa extract

A technology of Cistanche pipiensis and extract, which is applied to the new use field of Cistanche pipiensis extract, and can solve the problems of not specifically revealing Cistanche pipiensis and the like.

Inactive Publication Date: 2008-12-17
张小平 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in this patent, it is not specifically disclosed which aspect of the function of skin cells has been improved by Cistanche tubulosa

Method used

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  • Novel use of Cistanche tubulosa extract
  • Novel use of Cistanche tubulosa extract
  • Novel use of Cistanche tubulosa extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0210] The preparation of embodiment 1 Cistanche cistanche extract

[0211] 1. Cistanche 10 kg, crushed into coarse powder, add 8 times the amount of 50% ethanol, heat to above 50°C for extraction for 2 hours, pass the extract through a 100-mesh sieve, add 6 times the amount of 50% ethanol to heat and extract twice, extract Pass the liquid through a 100-mesh sieve; combine the extracts, concentrate to a specific gravity of 1.10 under the condition of vacuum degree ≥ 0.08Mpa, cool and precipitate for 12 hours, take the supernatant and centrifuge to obtain the centrifugate, inject it into the SEPABEADS resin column, add 2 times the amount Elute with 20% ethanol, collect the eluate, and then elute with 2 times the amount of 30% ethanol, repeat this process again, combine the ethanol eluate for 3 times, decompress and concentrate to obtain the extract containing phenylethanol glycosides 710 gram.

[0212] Determination of content of ergosteroside and echinacoside by high performa...

Embodiment 2

[0219] Embodiment 2 toxicity test

[0220] 1. Acute toxicity (oral LD 50 )test:

[0221] Objective: To observe the adverse reaction and death caused by oral administration of the test sample once to animals and to determine the median lethal dose.

[0222] Testing basis: GB15193.3-994

[0223] 1.1 Materials and methods:

[0224] 1.1.1 Sample name: Cistanche tuberosum extract.

[0225] 1.1.2 Sample properties: dark brown powder.

[0226] 1.1.3 Test sample preparation: Weigh 10000mg of sample, grind it, add distilled water to 20ml, mix well and prepare a homogeneous suspension as the test sample.

[0227] 1.1.4 Test animals:

[0228] Twenty Kunming mice, half male and half male, weighing 18-22 grams, were provided by the Experimental Animal Department of Fudan University.

[0229] Certificate number: 02-22-1. Breeding room temperature: 18-22°C, relative humidity: 40-70%. Animal room certificate number: 02-28. The mouse feed was provided by Suhang Experimental Animal Scie...

Embodiment 3

[0361] Embodiment 3 stimulation test

[0362] 1), 6 guinea pigs, weighing 250±10g, half male and half male. 24 hours before the administration, remove the hair on both sides of the guinea pig spine, which is 8 × 5cm 2 , Check after 24 hours to ensure no skin damage.

[0363] Adopt self-comparison of the left and right sides of the same body, the left side is coated with the test article (containing 2% of the present invention rich in phenylethanol glycosides Cistanche tubulosa extract and other cosmetic auxiliary materials) 1g, and the right side is coated with 1g of excipients. Formulation as a control, fixed with film plus gauze, adhesive tape. Each guinea pig was raised in separate cages. After giving the test product for 24 hours, the residual test product was washed away with warm water. After 1, 24, 48, and 72 hours after the test product was removed, it was observed with the naked eye and histopathological examination, and recorded whether there was erythema on the sm...

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Abstract

The invention relates to a cistanche tubulosa extract and discloses a new use of the cistanche tubulosa extract and the preparation thereof for promoting the generation of the elastic microfiber of skin and regaining the stretching and reversion function of the elastic fiber of skin. In addition, the invention further indicates that the cistanche tubulosa extract can be used as a raw material and an additive for improving the state of the elastic fiber of skin. Tests indicate that the preparation containing the cistanche tubulosa extract has no toxic and side effect on human body and no adverse allergic reaction. The cistanche tubulosa extract of the invention brings good news to people who expect to solve the problems related to the elastic fiber of skin.

Description

technical field [0001] The invention relates to an application of a plant extract, in particular to a new application of the extract of Cistanche tubulosa. Background technique [0002] With the improvement of people's living standards, women of all age groups in society are paying more and more attention to skin health. Delicate and flawless skin is the eternal pursuit and yearning of women. Cistanche deserticola is a well-known traditional Chinese medicine for invigorating the kidney, which has the effects of nourishing essence and qi and anti-aging. It has been used as a dual-purpose traditional Chinese medicine for more than 2,000 years in my country. [0003] In recent years, Japanese scholars have discovered that phenylethanol glycosides are the active ingredients of plants, and in vitro culture experiments have confirmed that phenylethanol glycosides promote the activity of M cells and form collagen fiber networks. (Tanimoto S et all. Yakugaku Zasshi 2006 Mar.126(3):...

Claims

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Application Information

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IPC IPC(8): A61K36/64A61K8/97A61K8/02A61K8/06A61P17/00A61Q19/00
CPCA61K36/64A61P17/00A61P17/18A61P43/00
Inventor 方宗仁张小平王海龙
Owner 张小平
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