Method of preparing glutathione freeze-dried powder

Abstract

The invention discloses a method for preparing glutathione freeze-dried powder, which relates to a method for preparing reduced glutathione (GSH) freeze-dried powder by a reverse micelle extraction method. The method takes bread dry yeast as a raw material to prepare extract containing GSH and a P204 reverse micelle solution first, then the GSH extract is extracted through the reverse micelle solution, then a sodium chloride solution is used to back-extract the reverse micelle solution after the extraction, and then a finished product is prepared through hyperfiltration and freeze drying. The method is simple and has mild preparation conditions, the back-extracted reverse micelle solution can be regenerated and utilized, the method has low production cost; in the prepared freeze-dried powder, the content of the GSH is high up to 95.2 percent, the recovery rate is high up to 86.3 percent, and a compound amino acid concentrated solution byproduct can be prepared. The GSH freeze-dried powder prepared by the method can be widely used as antioxidant preservatives and flavor additives in pharmaceutical raw materials and food industry, and can also be used as feed additives and bread yeast culture media and so on.

Description

Technical field [0001] The invention belongs to the technical field of glutathione preparation, and particularly relates to a method for preparing reduced glutathione freeze-dried powder by a reverse micelle extraction method. technical background [0002] Glutathione is a very important active tripeptide. There are two forms of reduced form and oxidized form. It is widely present in animals, plants and microorganisms. The main physiological functions are to resist free radical damage, detoxify, promote cell protein synthesis, participate in the transmethylation or propylamino reaction, and maintain the normal function of liver cells. Therefore, glutathione is widely used in the medical field, such as the treatment of liver disease, acute and chronic renal failure, cardiovascular disease, senile eye disease, diabetic nerve damage, liver protection and intestinal protection after tumor radiotherapy, etc.; glutathione Peptides are also widely used in the food industry, such as nood...

AI Technical Summary

Problems solved by technology

The main disadvantages of this method are: 1. produce a large amount of eluted waste liquid with strong acidity and alkalinity, which must be purified before being discharged, which not only increases the production cost, but also increases the process equipment and site for treating the waste liquid, thereby further increasing the production cost. Cost of production

②The crystallization process of this method often loses more products and leads to a decrease in yield

③ The prepared product is a crystal. When in use, the GSH crystal needs to be prepared into a GSH solution, and the utilization rate of the GSH crystal is reduced due to the incomplete dissolution of the GSH crystal.

This method is easy to operate, but the purity of GSH in the obtained extract is not high, and the content of GSH in the freeze-dried powder is only 9.0%.