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Preparation method of glycosylated nano-fibre for identifying proteinicsubstance and applications

A nanofiber and glycosylation technology, which is applied to the preparation method of peptides, chemical instruments and methods, fiber treatment, etc., can solve the problems of restricting wide application and complex synthesis process of unsaturated sugar monomers, and achieve simple and easy preparation. Industrialized production, good stability and durability

Inactive Publication Date: 2008-10-15
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation of glycosylated nanofibers mainly contains two approaches at present: one is to synthesize sugar-containing polymers by polymerizing sugar-containing unsaturated monomers, and then obtain them by electrospinning (CN 1843592). This method can be well Combining the advantages of nanofibers with the functionality of sugar groups can be used for protein separation, but the synthesis process of unsaturated sugar monomers is relatively complicated, which limits its wide application to a certain extent; Sugar groups are fixed to the surface of nanofibers. In previous patents 200710070589.2 and 200810059174.X, we fixed chitosan polysaccharides and azide monosaccharides to the surface of acrylonitrile-based copolymer nanofiber membranes containing carboxyl groups by different methods , effectively verifying the feasibility of this method

Method used

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  • Preparation method of glycosylated nano-fibre for identifying proteinicsubstance and applications
  • Preparation method of glycosylated nano-fibre for identifying proteinicsubstance and applications
  • Preparation method of glycosylated nano-fibre for identifying proteinicsubstance and applications

Examples

Experimental program
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Effect test

Embodiment 1

[0025] The acrylonitrile / hydroxyethyl methacrylate copolymer (PANCHEMA, viscosity-average molecular weight is 80,000, hydroxyl molar content is 10.1%) that mass fraction is 6% is dissolved in dimethylformamide solvent, and spinning voltage is Electrospinning was carried out at 8 kV, the flow rate of the spinneret solution was 0.5 ml / hour, and the receiving distance was 15 cm, and PANCHEMA nanofibers with a diameter of 152 ± 24 nm were prepared.

[0026] 15 mg of PANCHEMA nanofibers are immersed in 15 milliliters of glucose pentaacetate in dichloromethane solution, the molar ratio of the amount of glucose pentaacetate to the surface hydroxyl of PANCHEMA nanofibers is 1, and boron trifluoride ether is added under nitrogen protection Complex compound, the molar ratio of its adding amount and used sugar monomer is 3. , shaking in an ice-water bath for 2 hours, then shaking and reacting at room temperature for 20 hours, washing and drying with acetone several times after the reacti...

Embodiment 2

[0028] The mass fraction is 6% PANCHEMA (viscosity-average molecular weight is 150,000, carboxyl molar content is 10.1%) is dissolved in the dimethylformamide solvent, is 15 kilovolts at spinning voltage, spinneret solution flow rate is 1.0 milliliters Electrospinning was carried out under the condition that the receiving distance was 15 centimeters per hour, and PANCHEMA nanofibers with a diameter of 367±28 nanometers were prepared.

[0029] 15 mg of PANCHEMA nanofibers were immersed in 15 milliliters of glucose pentaacetate in dichloromethane solution, the molar ratio of the amount of glucose pentaacetate to the surface hydroxyl of PANCHEMA nanofibers was 30, and boron trifluoride ether was added under nitrogen protection Complex compound, the molar ratio of its adding amount and used sugar monomer is 5. Shaking in an ice-water bath for 2 hours, then shaking and reacting at room temperature for 20 hours, washing and drying with acetone several times after the reaction, to ob...

Embodiment 3

[0031] The mass fraction is 4% PANCHEMA (viscosity-average molecular weight is 150,000, carboxyl molar content is 10.1%) is dissolved in the dimethyl sulfoxide solvent, is 15 kilovolts at spinning voltage, spinneret solution flow rate is 1.0 milliliters Electrospinning was carried out under the conditions of receiving distance of 8 cm per hour and a receiving distance of 8 cm to prepare PANCHEMA nanofibers with a diameter of 205 ± 26 nanometers.

[0032] 15 mg of PANCHEMA nanofibers were immersed in 15 milliliters of glucose pentaacetate in dichloromethane solution, the molar ratio of the amount of glucose pentaacetate to the surface hydroxyl of PANCHEMA nanofibers was 30, and boron trifluoride ether was added under nitrogen protection Complex compound, the molar ratio of its adding amount and used sugar monomer is 5. Shaking in an ice-water bath for 2 hours, then shaking and reacting at room temperature for 20 hours, washing and drying with acetone several times after the rea...

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Abstract

The invention discloses a method for preparing glycosylation nanometer fiber for protein recognition and applying the same. The method comprises the following steps that: acrylonitrile group copolymer is used as a raw material; acrylonitrile group copolymer nanometer fiber with controllable diameter is prepared by means of electrostatic spinning; sugar monomer which is protected by acetyl is fixed on the surface of nanometer fiber under the catalysis of boron trifluoride etherate; acetyl group is stripped down with methanol solution of sodium methoxide; and affinity nanometer fiber for glycosylation is obtained. With high specific surface area of the nanometer fiber, high porosity and a special biological function of glycosyl, the glycosylation nanometer fiber with a high immobilization rate can be immersed directly into protein solution, or the glycosylation nanometer fiber is superposed in multilayer to arrange in a membrane exchange filter, which is used for separation and purification of the protein. The glycosylation nanometer fiber for protein recognition has the advantages of simple preparation method and, repeatable use for many times and is favorable for industrialization production and so on.

Description

technical field [0001] The invention relates to a preparation method of nanofibers, in particular to a preparation method and application of glycosylated nanofibers for protein recognition. Background technique [0002] Molecular recognition is a ubiquitous phenomenon in the entire biological world. It mainly refers to the selection and combination of host molecules to their complementary guest molecules depending on their shape, size and chemical function. This phenomenon plays an extremely important role in the life process. Such as the interaction between enzyme and substrate, antigen and antibody. More than 100 years ago, Fisher described this specific combination between molecules with the cooperation of "lock and key". It is an important chemical concept to study biological phenomena such as enzymatic reaction, information transfer, and energy transfer between different media at the molecular level. Among them, the molecular recognition of proteins has become one of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D01F11/06D01F6/40D01D5/00C07K1/14
Inventor 徐志康车爱馥万灵书
Owner ZHEJIANG UNIV
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