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Antiviral polypeptides and use

An anti-virus and measles virus technology, applied in the field of anti-measles virus and human immunodeficiency virus anti-virus polypeptide of East Asian scorpion, and the field of high-efficiency anti-virus function polypeptide, which can solve the instability of its own genetic material and the reduction of preventive effect , prone to mutations and other problems, to achieve the effect of good water solubility, good inhibitory effect, and no side effects

Inactive Publication Date: 2008-10-15
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, some viruses, such as influenza and HIV, have unstable genetic material and are prone to mutations, so that the preventive effect of previous vaccines against this new mutant virus is reduced or completely ineffective.

Method used

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  • Antiviral polypeptides and use
  • Antiviral polypeptides and use
  • Antiviral polypeptides and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Preparation of a scorpion venom antiviral polypeptide. Proceed as follows:

[0026] A: Extraction of total RNA from scorpion venom glands (Trizol LS one-step method: Trizol LS was purchased from Invitrogen, USA)

[0027] ① Grind 500mg of scorpion glands into fine powder in liquid nitrogen, add 10ml Trizol LS to mix, and place at room temperature (20-25°C, the same below) for 5 minutes; Minutes, centrifuge at 12,000g for 15 minutes at 4°C; ③Add 1 volume of isopropanol to the water phase, place at room temperature for 10 minutes, and centrifuge at 12,000g for 10 minutes at 4°C to obtain RNA precipitation; ④Wash the precipitate with 5ml of 75% ethanol, and centrifuge at 7,500g for 5 minutes ; ⑤ After the RNA precipitate was dried, it was dissolved in DEPC (diethypyrocarbonate, diethyl pyrocarbonate)-treated sterilized water, and kept at 55-60°C for 10 minutes to completely dissolve the RNA. The whole process was carried out according to the method recommended ...

Embodiment 2

[0075] Embodiment 2: chemical synthesis of scorpion venom antiviral polypeptide AVP-W2

[0076] A polypeptide AVP-W2 consisting of 17 amino acids was isolated from the venom gland of the East Asian scorpion and was amidated. A high-purity AVP-W2 polypeptide: LVGLLPSVIGGLVSAFK-NH2 was obtained by solid-phase chemical synthesis.

Embodiment 3

[0077] Example 3: Inhibition of Measles Virus by Antiviral Polypeptide AVP-W2 Drugs

[0078] Material:

[0079] A: Culture of Vero E6 cells

[0080] The African green monkey kidney epithelial cell line (VeroE6) was purchased from China Center for Type Culture Collection.

[0081] Medium: 10% (passage medium) or 2% (maintenance medium) calf serum (FCS, GIBCO-BRL), 56 ° C, 30 minutes heat sterilization; 2mM / mL L-glutamate; 100U / mL Penicillin and 100 μg / mL streptomycin. Culture conditions are: 37°C, 5% CO 2 Incubator cultivation.

[0082] B: virus strain

[0083] The Edmonston measles virus used in this study came from the Wuhan Institute of Virology, Chinese Academy of Sciences (purchased from the American Type Culture Collection).

[0084] Specific operation method:

[0085] Monolayer Vero E6 cells were inoculated in a 96-well plate, 10 per well 4 For cells, add resistant medium to allow the cells to grow and adhere to the wall. The virus was added to the maintenance m...

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Abstract

The invention discloses scorpion toxin antiviral polypeptide and a use of the same. Scorpion toxin antivirotic polypeptide (AVP-W2) is obtained through a molecular biological and chemical synthesis method, and then the antiviral activity of the AVP-W2 on measles virus (MeV) and human immunodeficiency virus (HIV) is measured through adopting an antibody neutralization method; moreover, the measurement shows that the AVP-W2 can effectively inhibit virus infection at a low concentration. The AVP-W2 has a tremendous prospect in making applied medicine which can cure or prevent a disease caused by virus. Moreover, the antiviral peptide of the invention has high activity on MeV and HIV and a simple method, and can be developed as antiviral medicine.

Description

technical field [0001] The invention belongs to the field of biotechnology, and more specifically relates to a polypeptide with high-efficiency antiviral function. Specifically, the present invention relates to the use of an antiviral polypeptide of the scorpion scorpion in anti-measles virus (MeV) and human immunodeficiency virus (HIV). Background technique [0002] Virus infection is a disease that seriously endangers human health, and this problem has plagued human beings for many years. As early as 412 BC in ancient Greece, Hippocrates had already described influenza-like diseases; from 1742 to 1743, influenza caused by influenza viruses had affected 90% of Eastern Europeans; from 1889 to 1894 The "Russian flu" that swept through Western Europe in 1950 had a wide range of morbidity and a high mortality rate; in 1918, a deadly flu swept the world, causing 20 to 50 million deaths; the "Asian flu" in 1957 and the "Hong Kong flu" in 1968 According to statistics in the Unit...

Claims

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Application Information

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IPC IPC(8): C07K7/08C12N15/12A61K38/10A61P31/14A61P31/18
Inventor 李文鑫曹志贱戴潮鄢慧民赵震环马一保
Owner WUHAN UNIV
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