Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

DNA sensitivity electrode modified by hydrotalcite nanometer slice and preparation thereof

A sensitive electrode and hydrotalcite technology, applied in the direction of material analysis, instruments, and measuring devices through electromagnetic means, can solve the problems of easy leakage, anti-interference, and small amount of DNA immobilization, and achieve wide linear response range and strong anti-interference The effect of resistance and wide pH value application range

Inactive Publication Date: 2008-10-08
BEIJING UNIV OF CHEM TECH
View PDF0 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, electrochemical DNA sensors have problems such as small amount of DNA immobilization, easy leakage and poor anti-interference.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • DNA sensitivity electrode modified by hydrotalcite nanometer slice and preparation thereof
  • DNA sensitivity electrode modified by hydrotalcite nanometer slice and preparation thereof
  • DNA sensitivity electrode modified by hydrotalcite nanometer slice and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Preparation of hydrotalcite nanosheet sol

[0031] Weigh 0.12mol of Co(NO 3 ) 2 ·6H 2 O and 0.06mol of Al(NO 3 ) 3 9H 2Dissolve O in 100mL deionized water to make a salt solution; weigh 0.35mol of NaOH and dissolve it in 100mL deionized water to make an alkaline solution. Add the alkali solution and the salt solution into a colloid mill with a rotation speed of 400 rpm and mix them back for 2 minutes to obtain a pink suspension; quickly transfer the suspension to a four-necked flask, and crystallize at 100°C under the protection of nitrogen After 6 hours, filter with suction, wash until the pH of the filtrate is 6, then put the filter cake in an oven at 50°C to dry for 48 hours, and grind to obtain cobalt-aluminum hydrotalcite powder with a Co / Al molar ratio of 2:1. Take 0.025 g of the cobalt-aluminum hydrotalcite, add it to 50 mL of formamide, and stir at a constant temperature of 90° C. for 0.5 hour on a magnetic stirrer to obtain a hydrotalcite nanosheet so...

Embodiment 2

[0036] (1) Preparation of hydrotalcite nanosheet sol

[0037] Weigh 0.12mol of Co(NO 3 ) 2 ·6H 2 O and 0.04mol of Al(NO 3 ) 3 9H 2 Dissolve O in 100mL deionized water to make a salt solution; weigh 0.31mol of NaOH and dissolve it in 100mL deionized water to make an alkaline solution. Add the alkali solution and the salt solution into a colloid mill with a rotation speed of 700 rpm and mix them back for 1.5 minutes to obtain a pink suspension; quickly transfer the suspension to a four-necked flask, and crystallize at 90°C under nitrogen protection After 7 hours, filter with suction, wash until the pH of the filtrate is 6.5, then put the filter cake in an oven to dry at 70°C for 24 hours, and grind to obtain cobalt-aluminum hydrotalcite powder with a Co / Al molar ratio of 3:1. Take 0.05 g of the cobalt-aluminum hydrotalcite, add it to 50 mL of formamide, and stir at a constant temperature of 80° C. for 2 hours on a magnetic stirrer to obtain a hydrotalcite nanosheet sol wit...

Embodiment 3

[0042] (1) Preparation of hydrotalcite nanosheet sol

[0043] Weigh 0.12mol of Co(NO 3 ) 2 ·6H 2 O and 0.03mol of Al(NO 3 ) 3 9H 2 Dissolve O in 100mL deionized water to make a salt solution; weigh 0.29mol of NaOH and dissolve it in 100mL deionized water to make an alkaline solution. Add the alkali solution and the salt solution into a colloid mill with a rotation speed of 1000 rpm and mix them back for 1 minute to obtain a pink suspension; quickly transfer the suspension to a four-necked flask, and crystallize at 95°C under the protection of nitrogen After 8 hours, filter with suction, wash until the pH of the filtrate is 7, then put the filter cake in an oven and dry at 80°C for 12 hours, and grind to obtain cobalt-aluminum hydrotalcite powder with a Co / Al molar ratio of 4:1. Take 0.1 g of the cobalt-aluminum hydrotalcite, add it to 50 mL of formamide, and stir at a constant temperature of 70° C. for 3 hours on a magnetic stirrer to obtain a hydrotalcite nanosheet sol ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a DNA sensitive electrode decorated by hydrotalcite nano-chip and the preparation method thereof, which belongs to the field of electrochemical biosensors and preparation technology thereof. The sensitive electrode uses a glassy carbon electrode as the substrate electrode, and a sensitive film composed of double-strand DNA and co-aluminum hydrotalcite nano-chip is decorated on the surface of the substrate electrode. The preparation method of the sensitive electrode is as follows: the co-aluminum hydrotalcite nano-chip collosol is coated on the surface of the clean glassy carbon electrode; after dried at room temperature, the glassy carbon electrode is washed with second distilled water and dried, then the double-strand DNA solution is dropped on the surface of the hydrotalcite nano-chip; after dried at room temperature, the glassy carbon electrode is washed with second distilled water and dried, then the DNA sensitive electrode decorated by hydrotalcite nano-chip is prepared. The sensitive electrode has the advantages that the invention uses the properties of the hydrotalcite nano-chip, that is, the hydrotalcite nano-chip has large surface area, has high-density positive charge, has a lot of hydroxide radical on the surface and has good electric conductibility and so on, to realize the stability and fixing of mass DNA, and the electrode can be used in quantitative determination of phenol, which has good anti-interference performance.

Description

technical field [0001] The invention belongs to the technical field of electrochemical biosensors and preparation thereof, in particular to a DNA sensitive electrode modified by hydrotalcite nanosheets and a preparation method thereof. Background technique [0002] Electrochemical biosensors have the characteristics of stable performance, low price, easy operation, and easy miniaturization, and have been widely used in clinical diagnosis, industrial control, food testing, drug analysis, environmental analysis, biochips, and military fields. Among them, electrochemical DNA sensors have attracted much attention in recent years. They are not limited to the testing of gene sequences, but have also been greatly developed in drug analysis and pollutant testing. They are currently a hot spot in the field of electrochemical biosensor research. As in the literature (1) Biosensors and Bioelectronics, 2006, 21: 1777-1783, Kavita Arora et al. used the electrodeposition method to form a ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N27/327
Inventor 杨文胜李立群王毅
Owner BEIJING UNIV OF CHEM TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com