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Production method for microbial polysaccharide welan gum

A production method, sugar welan gum technology, applied in the field of bioengineering, can solve the problems of increasing investment cost and production cost, high energy consumption, etc., and achieve the effect of promoting green production, reducing pollution, reducing investment cost and production cost

Inactive Publication Date: 2008-10-01
安徽龙津生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The organic solvent used needs to be rectified and recycled, but it consumes a lot of energy. The production workshop uses organic solvents, and there are explosion-proof requirements in the design and production process, which increases its investment cost and production cost.

Method used

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  • Production method for microbial polysaccharide welan gum

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Embodiment 1

[0024] Embodiment 1. the production of microbial polysaccharide welan gum

[0025] Strains Alcaligenes ATCC 31555.

[0026] The seed medium used is (g / L): glucose 10, ammonium nitrate 0.6, yeast powder 2.5, peptone 5, KH 2 PO 4 0.5, MgSO 4 0.1, pH6.8, sterilized at 121°C for 20min.

[0027] Fermentation medium used (g / L): glucose 10, sucrose 1, yeast powder 1.0, urea 1, dipotassium hydrogen phosphate 0.5, MgSO 4 0.1, pH 6.0, sterilized at 121°C for 20min.

[0028] The feed medium used (g / L): glucose 400, yeast powder 10, urea 40, and ammonia water 12.5% ​​(v / v).

[0029] Fermentation was carried out on a 15L Biostat C10-3 mechanical stirring tank, the inoculum size was 5% (v / v), the liquid filling volume was 9L, the stirring speed of the fermenter was 500r / min, the ventilation intensity was 1.0vvm, and the tank pressure was maintained at 0.01MPa. The temperature is 30° C., and the pH value of the fermentation broth is controlled by feeding medium, and the pH is contro...

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Abstract

The present invention provides a preparing method of microbial polysaccharides Weilan gum, belonging to biotechnology field. The invention use an alcaligenes faecalis as a fermentation strain, using sucrose or glucose commonly used in food industry as carbon source for microbe cultivation, using conventional chemical fertilizer in the agricultural production as nitrogen source and inorganic salt, the medium of microbial growth is prepared by auxiliary yeast powder, aerobic fermentation is processed in the conditions of 30 DEG C, 1.0vvm, 500r / min ventilatiing and stirring, adding specially supplementary food fluid in the fermentation course achieves the following aims: (1) controlling the penetrating pressure of the broth in the hypotonicity condition; (2) controlling the pH of the broth between 4.0 and 7.0; (3) providing abundant nutritious matter such as carbon source and nitrogen source for microbial growth, obtaining Weilan gum broth after 72 hours, obtainin Weilan gum product by drying in 95-115 DEG C and atmospheric pressure. The production process of the Weilan gum product does not use organic solvent, the broth obtains Weilan gum product to correspond with the petroleum exploitation industry standard only processing one step drying.

Description

technical field [0001] The invention discloses a method for producing microbial polysaccharide welan gum, which belongs to the technical field of bioengineering. Background technique [0002] Welan gum (welan gum) is usually produced by fermentation of a Gram-negative rod-shaped Alcaligenes. It can be used as an excellent stabilizer, thickener, suspending agent, gelling agent, etc., mainly used in geological and mineral resources and construction industries. Its molecular structure is shown below, the main chain is composed of glucose, glucuronic acid and rhamnose units, the ratio is 2:1:1, about every 4 units have a rhamnose or mannose with α-(1, 3) The glycosidic bond branches are connected to the gluconic acid unit, and 85% of the repeating units have an acetyl group connected to the 2-carbon of β-(1,3)glucose. [0003] [0004] Molecular structure of welan gum [0005] As an excellent profile control agent, welan gum has good thickening properties...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12N1/20C12R1/05
Inventor 詹晓北郑志永贾薇朱莉吴剑荣
Owner 安徽龙津生物科技有限公司
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