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A kind of biocontrol actinomycetes strain and application thereof for preventing and treating tobacco bacterial wilt

A technology of actinomycetes and biocontrol agents, applied in the field of microorganisms, can solve the problems of lack of high-efficiency strains, weak research foundation of tobacco bacterial wilt disease, etc., and achieve the effect of reducing agricultural pollution

Inactive Publication Date: 2017-08-29
INST OF PLANT PROTECTION FAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But generally speaking, the research foundation of using antagonistic strains to control tobacco bacterial wilt in my country is still relatively weak, especially the obvious lack of effective strains

Method used

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  • A kind of biocontrol actinomycetes strain and application thereof for preventing and treating tobacco bacterial wilt
  • A kind of biocontrol actinomycetes strain and application thereof for preventing and treating tobacco bacterial wilt
  • A kind of biocontrol actinomycetes strain and application thereof for preventing and treating tobacco bacterial wilt

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Screening of antagonistic actinomycetes

[0024] The specific process is as follows:

[0025] 1. Collection of soil samples

[0026] The rhizosphere soil at a depth of 5-20 cm from different tobacco field plots was collected from Zhenghe County, Fujian Province, a total of 4 parts were packaged and marked and brought back to the laboratory, and separated after natural air-drying.

[0027] 2. Isolation of actinomycetes

[0028] Separation was performed using the plate dilution method. Weigh 10g of the soil sample, pour it into a conical flask equipped with small glass beads and 90ml of sterile water, shake it for 30min, then let it stand for 5 minutes, dilute it by 10 times successively, and prepare 10 -2 、10 -3 、10 -4 、10 -5 、10 -6 0.1ml of the suspension of different concentrations was added to Gao's No. 1 medium (adding K with a final concentration of 50mg / L 2 GrO 7 ) plate, spread evenly and place it at 28°C for observation. After 5-7 days, differe...

Embodiment 2

[0034] The mensuration of embodiment two fermented liquid antibacterial activity

[0035] Six strains of actinomycetes whose inhibition zone diameters were all above 30mm were re-screened by cup and saucer method. The strains were inoculated into the seed medium, and after 2 days of fermentation in shake flasks at 28°C, they were inserted into the fermentation medium with 5% inoculum, cultured at 28°C and 200 r / min for 6 days, and the fermentation broth was centrifuged at 8000 r / min for 5 min. A sterile fermentation broth is obtained after filtration with a bacterial filter. Tobacco R. solanacearum (10 8 CFU / ml) in the center of the NA plate, 100 μl of sterile fermentation broth was added to the hole, cultured at 28°C for 2 days, and the diameter of the inhibition zone was measured, repeated 3 times. The results showed (Table 2) that the inhibition zones of strains P12 and K4 were both above 20 mm, and the K4 fermentation broth had the strongest antibacterial effect, with th...

Embodiment 3

[0039] The identification of embodiment three bacterial strains K4

[0040] 1. Observation of morphological characteristics

[0041] Streak inoculation of the strain K4 on Gao’s No. 1 medium, insert a sterile cover glass at an angle of 45 degrees, culture at 28°C for 7 days, take out the cover glass and observe the growth of hyphae and spores under an optical microscope and an electron microscope. Morphological characteristics. It was found that the spore filaments of strain K4 were helical, often in clusters, and the spore filaments were broken into spores, spores were oval, and the surface was rough ( figure 1 and figure 2 ).

[0042] 2. Observation of culture characteristics

[0043] Using the 8 kinds of media recommended by the Streptomyces Identification Manual, culture the strain K4 at 28°C for 7-10 days, observe the growth of the bacteria, record the color of the aerial hyphae and basal hyphae, and whether soluble pigments are produced. Strain K4 grew well on Gao's...

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Abstract

The invention discloses an actinomycete strain for preventing and treating tobacco bacterial wilt and an application method thereof, belonging to the technical field of microorganisms. The strain is Streptomyces sp. K4, which has been deposited in the General Microbiology Center of China Committee for the Collection of Microorganisms, with the date of preservation being March 24, 2014, and the preservation number is CGMCC NO.8949. The bacterial strain inhibits the growth of tobacco bacterial wilt by producing active substances, and shows better control effect on tobacco bacterial wilt. The biocontrol actinomycete strain is used to prepare a biocontrol agent, which can be used with root-fixing water when transplanting tobacco seedlings; or can be used after root irrigation 4-5 days after transplanting. The bacterial agent can also be mixed with organic fertilizer to make bacterial fertilizer. The strain K4 is obtained from the rhizosphere soil of the tobacco field, and is compatible with the soil ecology, so it has a good application prospect in the biological control of tobacco bacterial wilt.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to a biocontrol actinomycete strain for preventing and treating tobacco bacterial wilt and its application. Background technique [0002] Tobacco bacterial wilt is caused by the bacteria Ralstonia solanacearum ( Ralstonia solanacearum ) is a soil-borne disease that is widespread worldwide. The strains of the disease are complex, the range of hosts is wide, and it is very easy to break out and epidemic, so it is difficult to control. Tobacco bacterial wilt occurs commonly in the Yangtze River Basin and the areas south of my country, especially in Fujian, Guangdong, Hunan, Guizhou and other tobacco-growing areas, where the incidence rate of seriously diseased fields is as high as 90%, seriously affecting the yield and quality of tobacco. cause significant economic losses. [0003] At present, tobacco bacterial wilt is mainly prevented and controlled in production by cultivating...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P1/00
CPCA01N63/10C12N1/205C12R2001/465
Inventor 谢世勇丁雪玲谢廷鑫陈志厚林勇黄建成陈庆河李本金
Owner INST OF PLANT PROTECTION FAAS
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