Preparation of degradable pollutant polyalcohol stephanoporate microballoons and uses thereof

Abstract

A preparation method for porous microspheres of a biodegradable polymer is provided. The method comprises the following steps: (a) dissolving a biodegradable polymer in an organic solvent to form an oil phase of 1-30% (g / ml) concentration; (b) selecting a pore-forming agent 1-50% of the biodegradable polymer in the step (a) and dissolving the pore-forming agent in water to form an internal water phase; (c) adding dropwise the internal water phase into the oil phase at a volume ratio of (1-30):100, and stirring to form a primary emulsion; (d) preparing a polyvinyl alcohol aqueous solution of 0.5-10% (g / ml) concentration, which serves as an external water phase; (e) pouring the primary emulsion into the external water phase at a volume ratio of (3-30):100, stirring or performing ultrasonic treatment to form a double-emulsion, and allowing the polymer in the double-emulsion to solidify, thereby forming microspheres; (f) freeze-drying the microspheres to obtain the final product. The obtained porous microspheres of the biodegradable polymer have high porosity, large specific surface area and good adsorption and encapsulation properties; and are used for the adsorption and encapsulation of unstable protein drugs, polypeptide drugs and growth factors and can retain pharmaceutical activity and effectiveness thereof.

Description

technical field [0001] The invention relates to biomedical engineering, in particular to a method for preparing biodegradable polymer microspheres and the use of the prepared biodegradable polymer porous microspheres. Background technique [0002] With the development of biopharmaceutical technology, protein and peptide active drugs have advantages in recent years due to their good therapeutic effect, less toxic and side effects, and certain effects on disease prevention, clinical treatment and solving the problems of protein quantity deficiency and function loss in the body. The use of protein-based drugs is on the rise. However, protein drugs have the characteristics of large molecular weight and complex spatial structure, and are easily damaged by the physiological environment, especially enzymes, during drug delivery. In addition, its inherent weak penetration ability and natural instability determine its easy inactivation characteristics. Therefore, it is of great sig...

AI Technical Summary

Problems solved by technology

However, in the preparation process of this method, the degradable polymer and the drug are dissolved in the organic solvent at the same time, which has a great impact on the drug, especially the protein active drug, and even leads to the loss of drug activity, which greatly affects the protein and polypeptide active drug. curative effect; at the same time, the stirring in the process of adding the organic solvent makes the drug bear the effect of shear force, and the oil-water interface will also have an adverse effect on the drug properties of the drug, further reducing the drug properties of the drug