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Use of Polygonum cuspidatum extract for preparing anti-HIV and anti-hepatitis B virus products

A technology of Polygonum cuspidatum extract, HIV, applied in the direction of antiviral agents, medical preparations containing active ingredients, sugar derivatives, etc. Hepatitis virus, etc.

Inactive Publication Date: 2012-02-01
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0064] According to literature search, so far, no report on the use of these two compounds in the prevention, diagnosis, detection, protection, treatment and research of HIV and hepatitis B virus and their directly related diseases has been found.

Method used

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  • Use of Polygonum cuspidatum extract for preparing anti-HIV and anti-hepatitis B virus products

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0165] (1) preparation method of Polygonum cuspidatum extract

[0166] In order to further illustrate the extraction method of the above-mentioned Polygonum cuspidatum extract, especially compound 1 and compound 2, the following examples are used to illustrate.

[0167] For example, the preparation method of the said Polygonum cuspidatum extract of the present invention can be:

[0168] (1) Extraction: grind the medicinal material of Polygonum cuspidatum into coarse powder as usual, extract by percolation with 10-95% ethanol, and collect the percolation liquid of 5-30 times the volume of the medicinal material, which is the extract.

[0169] Or: thermally extract the pulverized Polygonum cuspidatum medicinal material with 20-95% ethanol as usual, and the volume volume of the solvent medium is about 4-8 times that of the crude drug for the first time; About 4 to 12 times of the crude drug; get the extract; filter and concentrate the extract, centrifuge or filter the concentrat...

Embodiment 1

[0185] Embodiment 1, anti-HIV test

[0186] C8166 cell model test materials, methods and steps for in vitro anti-HIV-1 activity of drugs

[0187] (1) Reagent

[0188] HEPES, MTT, DMF, penicillin (Penicillin), streptomycin sulfate (Streptomycin sulfate), glutamine (Glutamine) were all purchased from Sigma Company; 2-mercaptoethanol (2-ME, 2-Mercaptoethanol) was purchased from Bio-Rad Company product. RPMI-1640 and newborn calf serum are products of Gibco.

[0189] (2) culture medium

[0190] RPMI-1640 complete medium, containing 10% newborn calf serum, 2mM L-glutamine, 10mM HEPES, 50μM 2-mercaptoethanol, 100,000 IU penicillin, 100μg / ml streptomycin.

[0191] (3) cells and viruses

[0192] Human T lymphocyte cell line C8166 and HIV-1 experimental strain HIV-1 IIIB All were donated by UK Medical Research Council, AIDS Reagent Project. All cells and viruses were cultured in RPMI-1640 complete medium containing 15% calf serum. Prepare HIV-1 by conventional method IIIB , ti...

Embodiment 2

[0204] Embodiment 2, anti-hepatitis B virus test in vitro

[0205] (1) Materials and reagents

[0206] Cells: HepG2-2.2.15 of human liver cancer cells transfected by HBV-DNA clone, which was constructed in 1987 by The Mount Sinai Medical Center in the United States. Dimethyl sulfoxide (abbreviation: DMSO), kanamycin, and MTT (3-(4,5-dimethylthiazole-2)-2,5-diphenyltetrazolium bromide) were purchased from Sigma Company; RPMI 1640 medium and fetal bovine serum, products of Gibco, USA; ELISA HBsAg detection kit: products of Bio / Rad; viral DNA levels were detected with ABI Prism 7000 sequence detector (Applied Biosystems).

[0207] (2) Test method

[0208] The HepG2-2.2.15 (2*10 6 After one week of drug action, the copy number of HBV DNA in cells and the concentration of HBV surface antigen HBsAg were detected by quantitative PCR and Elisa method, respectively, to preliminarily screen the drugs with inhibitory effect on HBV. This study also observed the growth curve of HepG2-2...

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Abstract

The present invention relates to the use of Polygonum cuspidatum extract. In vitro anti-HIV tests show that resveratrol 3-O-β-D-glucoside 2″-sulfate sodium or potassium salt and resveratrol 3-O-β-D-glucoside 4 ′-Sulphate-based sodium or potassium salts have shown significant anti-hepatitis B virus activity in cell models and in vivo transgenic mouse models; resveratrol 3-O-β-D-glucoside 2″-sulfate Base sodium or potassium salt also has significant inhibitory effect on HIV. Viral infectious diseases, especially AIDS and hepatitis B, seriously threaten human health and life. Hepatitis B is one of the main diseases affecting human health. The present invention provides new anti-HIV and anti-hepatitis B virus drugs for research and development. The lead compound provides a new drug source for clinical use, and is of great value to the development and utilization of medicinal plant resources in my country.

Description

technical field [0001] The present invention relates to the field of medicine and food technology, in particular to the use of Chinese medicine extracts, more specifically to the use of Chinese medicine extracts of Polygonum cuspidatum for the preparation of anti-HIV and anti-hepatitis B virus products, and more specifically It is said that it involves Chinese medicine extract, Polygonum cuspidatum extract, resveratrol 3-O-β-D-glucoside 2″-sulfate sodium or potassium salt (abbreviation: compound 1) and resveratrol 3-O-β - the use of D-glucoside 4'-sulfate group sodium or potassium salt (abbreviation: compound 2) for the preparation of anti-HIV and anti-hepatitis B virus products. Background technique [0002] (1) Research overview of Polygonum cuspidatum [0003] Polygonum cuspidatum is the rhizome of Polygonum cuspidatum Sieb.et Zucc. in the family Polygonaceae. It is mostly born in valleys, streams or banks, and is a perennial shrub-like herb, more than 1 meter high. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7034A61K36/704A61P1/16A61P31/18A61P31/20C07H15/203A61K125/00
Inventor 肖凯郑永唐何晓文杨柳萌王云华蔺红伟宣利江徐亚明张黎明赵杰蔡建明
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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