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Function of tetrandrine in preparing liver scathing-prevention medicament

A technology of tetrandrine and liver injury, applied in the field of pharmacy

Inactive Publication Date: 2008-06-18
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, in the prior art, tetrandrine has not been used for the prevention and treatment of hepatitis.

Method used

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  • Function of tetrandrine in preparing liver scathing-prevention medicament
  • Function of tetrandrine in preparing liver scathing-prevention medicament
  • Function of tetrandrine in preparing liver scathing-prevention medicament

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0062] Preparation method of tetrandrine and pharmaceutically acceptable salt thereof

[0063] The preparation method of tetrandrine can adopt methods known to those skilled in the art, such as chemical synthesis or extraction from plants. Preferably, a method of extracting tetrandrine from plants can be used.

[0064] The main advantages of the present invention are:

[0065] (1) The new application of tetrandrine in the prevention and treatment of hepatitis or liver injury has been discovered for the first time. It has strong pharmacological effects and no toxic and side effects, and has excellent medicinal prospects.

[0066] (2) The tetrandrine can be directly extracted from plants, and has the advantages of low toxicity, convenient administration, abundant sources, low price and simple preparation process.

Embodiment 1

[0069] Example 1 Preparation and injection of tetrandrine

[0070] 1. Preparation of tetrandrine injection

[0071] Prepare tetrandrine injection, the method is as follows: take 100 mg of tetrandrine (purity above 98%) solid powder purchased from Shaanxi Huike Plant Development Co., Ltd., add 0.1M hydrochloric acid to dissolve, then add the solution formed after dissolution to Add it to PBS to a final concentration of 20mg / ml, and prepare tetrandrine injections.

[0072] 2. Experimental Grouping and Injection

[0073] C57b1 / 6 mice (purchased from Shanghai Slack Experimental Animal Co., Ltd.) were selected as the test objects, and the mice were randomly divided into groups of 3-5 mice, and the control group and tetrandrine treatment group 1 and Han Tetrandrine treatment group 2.

[0074] Among them, the control group injected mice intraperitoneally with PBS, the tetradrine treatment group 1 injected mice intraperitoneally with 25 mg / kg body weight of tetrandrine, and the tet...

Embodiment 2

[0077] The effect of embodiment 2 tetrandrine on the activity of alanine aminotransferase (ALT) in CIH mice

[0078] In this example, the mice were treated as described in Example 1. After all the mice were injected with ConA at a dose of 15 mg / kg through the tail vein for 17 hours, the mouse serum was taken to measure the activity of alanine aminotransferase. The serum alanine aminotransferase activity assay kit produced by Hua Medical Technology Co., Ltd. was operated according to the instructions. The brief steps are as follows: After the serum is properly diluted, take 25 microliters and add it to a 96-well plate, add 100 microliters of enzyme working solution, incubate at 37 degrees Celsius for 15 minutes, add 150 microliters of stop solution, and read at 492nm on a microplate reader, according to the kit provided Calculate the alanine aminotransferase activity value using the standard serum and the dilution factor.

[0079] The data are expressed in the form of mean ± s...

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Abstract

The invention discloses an application of tetrandrine, which is used to prepare combined substances for inhibiting the expressions of chemokines and to control liver injury. The invention firstly discovers the new application of the tetrandrine in controlling hepatitis or liver injury. The invention has strong pharmacological effects without side effect , and thus the invention has good prospect in medicine. Furthermore, the tetrandrine can be directly extracted from the plants and is convenient to be taken with advantages of low toxicity, rich source, low price and simple preparation process.

Description

technical field [0001] The invention belongs to the field of pharmacy, and specifically relates to the use of tetrandrine in the preparation of medicines for preventing and treating hepatitis and liver damage. Background technique [0002] Hepatitis is a major disease that seriously endangers human health. From the perspective of etiology, hepatitis is mainly divided into: viral hepatitis, alcoholic hepatitis, autoimmune hepatitis and so on. The incidence of hepatitis, especially viral hepatitis, in China continues to be relatively high. As far as hepatitis B is concerned, there are 2 billion people infected with hepatitis B virus in the world, of which 690 million are in China, and the hepatitis B virus surface antigen (HBsAg) is carried around the world. There are 350 million people affected by hepatitis B virus, one-third of which are in China; 750,000 people die from diseases caused by hepatitis B virus infection every year in the world, of which 280,000 people are from ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4748A61P1/16A61P31/14A61P31/20A61P43/00
Inventor 徐凌云冯德春
Owner SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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