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Total sesquiterpene lactone extract containing rich parthenolide and preparation method and application thereof

A technology of sesquiterpene lactone and parthenolide, which is applied in the field of preparation of plant active ingredients, can solve problems such as not suitable for industrial production, and achieve the effect of simple ingredients, clear active ingredients, and stable process

Inactive Publication Date: 2008-06-04
BEIJING SUNHO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above literatures on the separation of parthenolide were all obtained when studying phytochemical components. It is a basic separation research experiment and is not suitable for industrial production.
And so far there is no literature to disclose the research on the extraction and separation process of parthenolide and the extraction and separation process of total sesquiterpene lactones rich in parthenolide from Magnolia magnolia

Method used

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  • Total sesquiterpene lactone extract containing rich parthenolide and preparation method and application thereof
  • Total sesquiterpene lactone extract containing rich parthenolide and preparation method and application thereof
  • Total sesquiterpene lactone extract containing rich parthenolide and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The preparation of total sesquiterpene lactones: take 10kg of Magnolia glabra leaves, grind into coarse powder, soak twice with deionized water, soak for 24h and 12h respectively, filter, discard the water extract, dry the filter residue, and use 95% Ethanol percolation extraction 3 times, the amount of solvent is 8 times the amount of medicinal materials, combined ethanol extracts, concentrated under reduced pressure at 60 ° C to the extract form, add 30% ethanol 3L reflux to make the extract into a suspension and dissolve, with Petroleum ether was extracted three times, the amount of petroleum ether was 3000mL each time, the petroleum ether extract was combined, the petroleum ether solvent was recovered under reduced pressure at 40°C to about 2000mL, the petroleum ether was back-extracted twice with 80% ethanol, 1000mL each time, and the ethanol was combined Add the extract into the alcohol-water mixture, concentrate under reduced pressure at 60°C, add 1L of 8% ethanol...

Embodiment 2～5

[0027] Implementation 2 to 5 is the preparation of total sesquiterpene lactones, 2 and 3 are respectively the examples extracted with methanol and acetone, and the rest are the same as in Example 1; 4 and 5 are respectively the implementation of turning chloroform into ethyl acetate and diethyl ether Example, all the other are identical with embodiment 1.

Embodiment 6

[0029]Take 50g from the 210.35g total sesquiterpene lactones prepared in the above example 1, dissolve in ethanol, mix the sample with 30-60 mesh silica gel, weigh another 2kg of silica gel (200-300 mesh), and put it into a glass column by dry method , the column inner diameter × length is 80 × 100cm, the silica gel mixed sample is installed on the clean silica gel, and a layer of clean silica gel and absorbent cotton is covered on the sample, and the mobile phase petroleum ether-ethyl acetate 10:1 is used for elution, until the washing The color of dehydration becomes light and ends when no desired components come down, then elute with petroleum ether-ethyl acetate 3:1, follow-up detection by UV, TLC, and HPLC, collect fractions containing parthenolide, combine them, and store them at 50°C Recover the solvent under reduced pressure to dryness, dissolve with as little methanol as possible, and go to C 18 Reverse-phase silica gel column, column inner diameter × length: 60 × 800...

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Abstract

The invention relates to a total sesquiterpene lactone extract containing rich parthenolide and the preparation method and application thereof. Particularly, the invention relates to a method of extracting sesquiterpene lactone containing rich parithenolide from yulan magnolia leaves and / or flower; yulan magnolia leaves and / or flower are taken as raw materials, crushed, extracted by polarity organic solvent and non-polarity organic solvent so as to get yulan magnolia total lactone; the yulan magnolia total lactone is processed with columnchromatography, and then the invention extract is obtained by collecting necessary components. The invention has definite effective components, the content of which is high, and is suitable for industrialized production. The invention has the anti-tumor virus function. The invention can be prepared into various pharmaceutical preparations with anti-tumor function.

Description

technical field [0001] The invention belongs to the field of preparation of plant active ingredients, and in particular relates to an extraction and separation method for extracting and separating total sesquiterpene lactone compounds rich in parthenolide from Magnolia magnolia. The invention also relates to the application of the extract in pharmacy and antineoplastic drugs. Background technique [0002] Magnolia is one of the important taxonomic groups in Magnoliaceae (Magnoliaceae). It is an excellent species of fast-growing forests, special economic forests, soil and water conservation forests, and urban and rural landscape ecological forests. It has great prospects for development and utilization. The dried flower buds of Magnolia biondii Pamp., Magnolia denudate desr. or Wudang Magnolia sprengeri Pamp. are the medicinal parts of Magnolia magnolia, which are clinically used to treat colds such as wind-cold headaches and nasal congestion. symptom. Literature (Zhao Liqi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/575C07D307/93C07D493/04C07D303/00A61P35/00A61K133/00
Inventor 张树祥熊国裕
Owner BEIJING SUNHO PHARMA
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