Pseudorabies virus SA215, pseudorabies virus polygene deletion bacterin and preparation method thereof

Abstract

The invention belongs to the technical field of animal virology and genetic engineering, relating to pseudorabies virus which lacks parts of virulence gene of min strain A of pseudorabies virus, and the process for preparing vaccine with the virus. The DNA recombinant technology is employed to artificially remove all or parts of albumen code area of a plurality of genes of TK, gE, gI, 11K, and 28k which are unnecessary genes and duplicate virus of viruses and influence the virulence in the pseudorabies virus, thereby lowering the virulence of the pseudorabies virus, achieving attenuated vaccine strains SA215 of which the attenuation genetic engineering is lack, preparing vaccine with SA215 virus, and immunizing animals to effectively prevent and control the occurrence and prevalence of the pseudorabies.

Description

Pseudorabies virus SA215 and pseudorabies virus multigene deletion vaccine and preparation method thereof Technical field: The invention belongs to the technical field of animal virology and genetic engineering, and relates to a pseudorabies virus lacking partial virulence genes of the Fujian A strain of pseudorabies virus and a method for preparing a vaccine with the virus. Background technique: Pseudorabies (Pseudorabies, PR) is caused by the herpesviridae, α-herpesvirus subfamily of pseudorabies virus (PseudorabiesVirus, PRV), a variety of livestock and wild animals with fever, itching and encephalomyelitis as the main symptoms An acute infectious disease. Pigs are the primary infected host of this disease, and they are also the long-term storage and detoxification of the virus. The occurrence and prevalence of the disease have two main features: a large number of piglets within two weeks of age with neurological symptoms and death, and maternal reproductive disorders ...

AI Technical Summary

Problems solved by technology

In order to overcome technical problems such as poor immunogenicity of existing vaccines, low safety, and difficulty in distinguishing wild virus-infected pigs from vaccine-immunized pigs, the present inventors used the Fujian A strain of pseudorabies virus as the original material, through homologous recombination Methods Several genes affecting virulence in Pseudorabies virus, TK, gE, gl, 11K, 28K, were artificially deleted, and a strain of SA215 virus with reduced virulence and no effect on the immunogenicity of Pseudorabies virus was obtained

Images