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Method for preparing microorganism grease by fermenting steam-exploded straw hemicellulose hydrolysate

A microbial oil and hemicellulose technology, applied in the field of microbial fermentation, can solve problems such as high production costs, achieve low prices, increase oil production, and reduce production costs

Inactive Publication Date: 2008-03-26
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, microbial oil is mainly produced by liquid fermentation of grain or molasses, and the production cost is high. Therefore, it is imperative to find new fermentation methods and abundant and cheap fermentation raw materials to produce microbial oil.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Take 200g of wheat straw and heat it under 200°C water vapor for 5min, release it rapidly from the reactor and explode it, soak it in 1L of tap water for 30min, filter to obtain 1L of steam-exploded wheat straw hemicellulose hydrolyzate, divide it into ten 500ml Erlenmeyer flasks 100ml per bottle, sterilized at 121°C for 20min, 5ml of Mortierella ramanii seed liquid into each bottle, cultured on a shaker at 30°C, 200r / min for 7d, collected mycelium, washed fully with water, and dried to obtain Dried thallus; reflux the obtained dry thallus with an excess mixed solvent composed of chloroform and methanol with a volume ratio of 2:1 in a Soxhlet extractor at 80°C for 6 hours, and remove the solvent under reduced pressure with a rotary evaporator to obtain oil , Obtained 8.9g of fats and oils altogether.

Embodiment 2

[0023] Take 200g of corn stalks and heat them under 220°C water vapor for 4 minutes, then release them rapidly from the reactor for explosion, soak them in 1L of tap water for 30 minutes, filter to obtain 1 liter of hemicellulose hydrolyzate of steam-exploded corn stalks, and use ten 500ml triangular Pack in separate bottles, 100ml per bottle, sterilize at 121°C for 20min, insert 5ml of Microspaeropsissp. seed solution into each bottle, culture at 30°C, 200r / min shaker for 6d, collect mycelia and wash fully with water, and dry to dry thalline; the excess mixed solvent that the gained dry thalline is formed by the volume ratio of 2: 1 chloroform and methanol is refluxed in Soxhlet extractor at 85 ℃ for 6 hours, removes solvent under reduced pressure with rotary evaporator, just obtains grease, A total of 7.6 g of oil was obtained.

Embodiment 3

[0025] Take 200g of wheat straw and heat it under 200°C water vapor for 5 minutes, release it rapidly from the reactor and explode it, soak it in 1L of tap water for 30 minutes, filter to obtain 1 liter of hemicellulose hydrolyzate of steam-exploded wheat straw, and use ten 500ml Erlenmeyer flasks Separate packaging, 100ml per bottle, sterilize at 121°C for 20min, add 5ml Mucor seed solution to each bottle, culture at 30°C, 200r / min shaker for 7d, collect the mycelium and fully wash with water, and dry to obtain dry bacteria body; the resulting dried bacteria was refluxed in a Soxhlet extractor at 85°C for 6 hours with an excess mixed solvent composed of chloroform and methanol with a volume ratio of 2:1, and the solvent was removed under reduced pressure with a rotary evaporator to obtain grease. 8.4 g of oil and fat were obtained.

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Abstract

The process of preparing microbial oil with steam blasted stalk hemicellullose hydrolysate includes the following steps: water soaking steam blasted stalk and filtering to obtain the steam blasted stalk hemicellullose hydrolysate, sterilizing, inoculating oil microbe seed liquid in 1-5 vol%, fermentation at 25-32 deg.c for 5-8 days, filtering to obtain mycelium and stoving, and extracting with organic solvent to obtain the microbial oil. The microbial oil containing high unsaturated fatty acid is applied in producing biodiesel oil. The present invention has best utilization of stalk material and low production cost.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, in particular to a method for preparing microbial oil by fermenting steam-exploded straw hemicellulose hydrolyzate. Background technique [0002] With the depletion of fossil energy and the continuous enhancement of people's environmental awareness, various renewable resources are getting more and more attention. Among them, biomass resources are the only renewable resources that can be converted into energy products and various chemical products. my country's straw production The material resources are very rich, and more than 700 million tons can be produced every year. Due to the very tight cell wall structure of plant straw, there are different binding forces between cellulose, hemicellulose and lignin molecules. The combination of cellulose and hemicellulose or lignin molecules mainly depends on hydrogen bonds; besides hydrogen bonds, there are also chemical bonds between hemicellulose ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/00C12P7/46C12P19/04C12R1/645C12R1/72C12R1/85
Inventor 陈洪章彭小伟
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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