Method for predicating tumor cell radiosusceptibilitiy by employing premature chromosome condensation

Abstract

The utility model mainly relates to a method to forecast the radiosensitivity of the tumor cells, in particular to a method to forecast the radiosensitivity of the tumor cells by utilizing premature chromosome condensation, which is characterized in the following steps: (1) to cultivate the cells and to start operation on the cells when the cells are in the log phase; (2) to add premature chromosome condensation inducer protein serine or tyrosine phosphatase inhibitor Calyculin A five minutes before irradiation; (3) to irradiate the cells according to different doses; (4) to measure the survival rate of the cells after irradiation with the colony formation method; (5) the trypsinization which is to collect the cells of different doses irradiation and to make chromosome samples of the premature chromosome by low porosity, fixed treatment and drop; (6) to count the result of the chromosome breakage and to fit the result of the chromosome breakage with the survival fraction of the cells so as to acquire the relationship between the chromosome breakage and the radiosensitivity of the cells. The utility model has the advantages of fast and accurate forecast of the radiosensitivity of the tumor cells, short consuming time of getting the result within twenty-four hours and little amount of work which indicates that the result can be derived by testing the type and number of the chromosome breakage in only forty cells in G2 phase.

Description

Technical field: [0001] The invention mainly relates to a method for predicting radiosensitivity of tumor cells, in particular to a method for predicting radiosensitivity of tumor cells by using precocious chromosome aggregation. Background technique: [0002] It has been reported abroad that the precocious chromosome agglutination technique is used to study the chromosomal damage of cells after radiation exposure, which is a fast and accurate research method. However, this method is only applied to the study of radiation-induced chromosomal damage, without clearly proposing the necessary relationship between chromosomal damage and cell radiation sensitivity, and there is no report on the efficiency of premature chromosome induction. [0003] Cancer is one of the diseases with a high mortality rate. Radiation biologists have made it a major goal to develop a method that can predict the radiation effects of tumors. Accurate prediction and analysis methods of tumor radiation...

AI Technical Summary

Problems solved by technology

But most of the research focuses on the cell G 2 period of research, for G 1 period and G 1 Period and G 2 There are few reports on the relationship between

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