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Method for predicating tumor cell radiosusceptibilitiy by employing premature chromosome condensation

A technology of tumor cells and chromosomes, applied in the field of predicting the radiation sensitivity of tumor cells, to achieve the effects of accurate radiation sensitivity, small workload, and short time-consuming

Inactive Publication Date: 2008-02-20
INST OF MODERN PHYSICS CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But most of the research focuses on the cell G 2 period of research, for G 1 period and G 1 Period and G 2 There are few reports on the relationship between

Method used

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  • Method for predicating tumor cell radiosusceptibilitiy by employing premature chromosome condensation
  • Method for predicating tumor cell radiosusceptibilitiy by employing premature chromosome condensation

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Embodiment 1

[0027] Embodiment 1: A method for predicting radiosensitivity of tumor cells by precocious chromosome agglutination has the following steps:

[0028] (1) Cultivate the cells and start operation when they are in the logarithmic growth phase;

[0029] Cell culture: Human liver cancer cell line SMMC-7721 (purchased from CCTCC) was incubated with RMPI-1640 medium containing 10% fetal bovine serum at 37°C, 5% CO 2 cultured in a constant temperature incubator.

[0030] (2) Add 50nmol / L precocious chromosome aggregation inducer protein serine / tyrosine phosphatase inhibitor Calyculin A 5 minutes before irradiation;

[0031] (2) irradiating the cells according to different doses;

[0032] Cell Irradiation: Human Hepatoma Cell SMMC-7721 in Exponential Growth Phase in the First Affiliated Hospital of Lanzhou Medical College 60 The γ-rays (dose rate 0.2Gy / min) produced by the Co source were irradiated.

[0033] (4) The survival rate of the cells after irradiation was measured by the clo...

Embodiment 2

[0037] The preparation method of the precocious chromosome aggregation inducer Calyculin A of the present invention comprises dissolving Calyculin-A in 100% ethanol to make a 1mM stock solution; before irradiation, it is added to the culture solution of cells to be irradiated, and the final concentration is 50nM.

Embodiment 3

[0039] The preparation method of the chromosome of the present invention, the cells are irradiated at 37 ° C, 5% CO 2 Continue to incubate for 30 minutes in a constant temperature incubator. Cells were collected, treated with 75mM KCl hypotonicity for 20 minutes, fixed with Carnot's solution, and finally suspended cells with a small amount of fixative solution, dropped into slices, dried on hot steam at 85-90°C, and stained with 5% Giemsa.

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Abstract

The utility model mainly relates to a method to forecast the radiosensitivity of the tumor cells, in particular to a method to forecast the radiosensitivity of the tumor cells by utilizing premature chromosome condensation, which is characterized in the following steps: (1) to cultivate the cells and to start operation on the cells when the cells are in the log phase; (2) to add premature chromosome condensation inducer protein serine or tyrosine phosphatase inhibitor Calyculin A five minutes before irradiation; (3) to irradiate the cells according to different doses; (4) to measure the survival rate of the cells after irradiation with the colony formation method; (5) the trypsinization which is to collect the cells of different doses irradiation and to make chromosome samples of the premature chromosome by low porosity, fixed treatment and drop; (6) to count the result of the chromosome breakage and to fit the result of the chromosome breakage with the survival fraction of the cells so as to acquire the relationship between the chromosome breakage and the radiosensitivity of the cells. The utility model has the advantages of fast and accurate forecast of the radiosensitivity of the tumor cells, short consuming time of getting the result within twenty-four hours and little amount of work which indicates that the result can be derived by testing the type and number of the chromosome breakage in only forty cells in G2 phase.

Description

Technical field: [0001] The invention mainly relates to a method for predicting radiosensitivity of tumor cells, in particular to a method for predicting radiosensitivity of tumor cells by using precocious chromosome aggregation. Background technique: [0002] It has been reported abroad that the precocious chromosome agglutination technique is used to study the chromosomal damage of cells after radiation exposure, which is a fast and accurate research method. However, this method is only applied to the study of radiation-induced chromosomal damage, without clearly proposing the necessary relationship between chromosomal damage and cell radiation sensitivity, and there is no report on the efficiency of premature chromosome induction. [0003] Cancer is one of the diseases with a high mortality rate. Radiation biologists have made it a major goal to develop a method that can predict the radiation effects of tumors. Accurate prediction and analysis methods of tumor radiation...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N33/48C12Q1/02
Inventor 杨建设李文建
Owner INST OF MODERN PHYSICS CHINESE ACADEMY OF SCI
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