Active constituent of curcuma longa for inhibiting and killing plant pathogenic fungi and its preparation and application
A technology of plant pathogenic fungi and active ingredients, which is applied in the field of plant pesticides and can solve the problems of plant pathogenic fungi causing great harm to plants and the like
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Embodiment 1
[0016] Preparation and antibacterial activity assay of embodiment 1 turmeric extract
[0017] Steps to prepare turmeric extract:
[0018] (1) The dried turmeric rhizome (or tuber) is crushed and passed through a 40-mesh sieve to obtain turmeric dry powder.
[0019] (2) Take 95% ethanol which is equivalent to about 7 times the volume of the raw material (the general control range is 5 to 10 times) and extract it at room temperature (the temperature is generally in the range of 18 to 24 ° C) for about 20 hours (the general control range is 12 ~24h).
[0020] (3) Continuous stirring and extraction in a constant temperature water bath at about 50°C (general control range is 45-60°C) for about 10h (general control range is 6-12h). Use a Buchner funnel to filter under negative pressure to remove the residue to obtain the filtrate ①.
[0021] (4) The residue is further agitated and extracted for 8 hours (the general control range is 6 to 12 hours) with 95% ethanol equivalent to ab...
Embodiment 2
[0032] The influence of embodiment 2 turmeric ethanol total extract, n-hexane extract on germination of pathogenic bacteria spores
[0033] (1) Bacteria to be tested: wasabi spp., olive green mold, Penicillium pallidum, Verticillium spp.
[0034] (2) Adopt the concave slide method: take the prepared drug-containing spore suspension (the final concentration of the drug solution is 10mg·ml -1 ) and 100 μl of the spore suspension of the blank control and the acetone control were dropped on the concave glass slide, put into a sterilized petri dish, and placed in a 26°C incubator for cultivation. Four replicates were set for the liquid treatment and the control group. . Check for spore germination after 12 hours. Microscopically inspect 10 fields of view, observe about 200 spores in each treatment, and calculate the spore germination rate. When the spore germ tube length exceeds half of the spore diameter length (minimum diameter), as the spore that has germinated, the calculati...
Embodiment 3
[0040] Example 3 Preparation and bioactivity determination of turmeric n-hexane extract.
[0041] (1) The total ethanol extract of turmeric was extracted and separated with 8 times the volume of n-hexane to obtain n-hexane extract, and the extract was concentrated with a rotary evaporator at a concentration temperature of about 40°C to obtain a paste-like extract.
[0042] (2) The above-mentioned extraction paste is configured with acetone solution to a concentration of 0.05g ml -1 The medicinal solution was used as a spare sample for the experiment. Wasabi sclerotium, rape sclerotium, tomato cinerea, Verticillium spp., wheat gibberella, edible fungus verruca, olive green mold, and Penicillium pallidum were used as the test bacteria, and the concentration of different medicinal solutions was determined. Antibacterial activity, antibacterial activity assay method is the same as embodiment 1.
[0043] The experimental results are shown in Table 3.
[0044] pathogenic...
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