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Aweto sporophore culturing process

The invention relates to a technology of Cordyceps militaris seeds and a cultivation method, which is applied to the cultivation field of medicinal edible fungus-Cordyceps militaris fruiting bodies, can solve the problem of low fruiting body transformation rate, and achieves the convenience of fresh body sales, high production speed and high biological efficiency. Effect

Inactive Publication Date: 2007-10-03
夏启华
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Problems solved by technology

Although these methods can cultivate fruiting body products, the conversion rate of fruiting body is not high

Method used

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Embodiment Construction

[0009] 1. Bacteria culture. Collect the spores of robust wild Cordyceps strains and inoculate them on PDA medium for sexual reproduction, and then purify and screen to obtain Cordyceps strains.

[0010] 2. Rejuvenation culture of strains. Inoculate the above-mentioned strains to the magnetized PSA medium added with 5% of the dry weight of the PSA medium's egg white for rejuvenation culture, purify and screen again, and remove the weaker and degenerated strains.

[0011] 3. Fruiting body cultivation. (1) Inoculation: Inoculate the rejuvenated mycelium of the strain into a transparent glass bottle or plastic bottle with a height of about 9-15cm and a diameter of about 5-10cm, and on the magnetized rice culture medium. Its composition is calculated by weight ratio: rice 52%, corn dregs 14%, pupa powder 26%, peptone 2%, beef 1%, glucose 4%, yeast 0.7%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.05% , 0.03% ammonium tartrate, 0.05% fumaric acid, 0.05% thiamine, 0.0...

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Abstract

The aweto sporophore culturing process includes three stages: spawn culturing, spawn rejuvenation and commercial sporophore culturing. The culture medium for rejuvenation culturing and the culture medium for sporophore culturing are magnetizing treated. The commercial sporophore culturing includes three steps: low temperature culturing, temperature difference culturing, and constant temperature culturing. The present invention adopts wild aweto spore as the propagation material to maintain the excellent features of wild aweto effectively; and the magnetizing treatment on the culture medium can excite the activity of culture medium, promote spawn growth and raise biological efficiency greatly.

Description

technical field [0001] The invention relates to a method for cultivating the fruiting body of Cordyceps militaris, a medicinal edible fungus. Background technique [0002] At present, the artificial cultivation of the fruiting bodies of Cordyceps militaris has been successful. Usually, the Cordyceps militaris collected in the field is inoculated on the strain culture medium to obtain strains, and then the strains are inoculated on the fruiting body culture medium, and cultured in the dark for several days before light culture. Such a method for artificially cultivating the fruiting bodies of Cordyceps militaris is disclosed in Chinese ZL99124200.9. And how to improve the yield and prevent the degradation of strains are the two main problems that need to be solved in the artificial large-scale cultivation of Cordyceps militaris at present. Generally, the yield can be increased by selecting a suitable medium and suitable process conditions, and ZL99124200.9 discloses just su...

Claims

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Application Information

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IPC IPC(8): C12N1/14
Inventor 夏启华
Owner 夏启华
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