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Method for separating preparing anthocyan monomer from mulberry

An anthocyanin and mulberry technology, which is applied in the preparation of sugar derivatives, chemical instruments and methods, and medical preparations containing active ingredients, etc., can solve the problems of large separation technology, small separation amount, difficult regeneration and use, etc. Preparation, low preparation cost, good separation effect

Inactive Publication Date: 2007-10-03
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The existing separation of anthocyanins mainly uses Sephadex LH-20 gel column chromatography and preparative high-performance liquid chromatography, the limitation of which is that the amount of separation is small
Secondly, high-performance liquid chromatography columns and Sephadex LH-20 are expensive and relatively difficult to regenerate and use. The cost of separating and obtaining anthocyanin monomers is relatively high, and it is difficult to develop into a separation technology with a large amount of preparation

Method used

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  • Method for separating preparing anthocyan monomer from mulberry
  • Method for separating preparing anthocyan monomer from mulberry
  • Method for separating preparing anthocyan monomer from mulberry

Examples

Experimental program
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Effect test

Embodiment 1

[0041] The solvent system of the present embodiment adopts TBME by volume: acetonitrile: n-butanol: TFA aqueous solution=1:1:3:5, the concentration of TFA aqueous solution is to contain 1 milliliter of TFA in every liter of aqueous solution, and the countercurrent chromatograph is HSCCC-D400 High-speed countercurrent chromatography, the content of anthocyanins in the crude extract of mulberry anthocyanins accounts for about 46%.

[0042] Measure 150ml of TBME, 150ml of acetonitrile, 450ml of n-butanol and 750ml of trifluoroacetic acid aqueous solution, put it in a 2000ml separatory funnel, shake it well, after standing for stratification, put the upper and lower phases into the reagent bottle respectively. The upper phase was injected into the chromatographic column of high-speed countercurrent chromatography at a flow rate of 20ml / min. Turn on the countercurrent chromatograph to 800 revs / min, then pump the sample solution (15ml lower phase solution with 0.6g mulberry anthocya...

Embodiment 2

[0044] The solvent system of the present embodiment adopts TBME by volume ratio: acetonitrile: n-butanol: TFA aqueous solution=2: 1: 2.5: 6, the concentration of TFA aqueous solution is to contain 5 milliliters of TFA in every liter of aqueous solution, countercurrent chromatograph is HSCCC-D400 High-speed countercurrent chromatography, the content of anthocyanins in the crude extract of mulberry anthocyanins accounts for about 46%.

[0045]Measure 300ml of TBME, 150ml of acetonitrile, 375ml of n-butanol and 900ml of TFA aqueous solution, put them in a 2000ml separatory funnel, shake them well, and put the upper and lower phases into reagent bottles respectively after standing for stratification. The upper phase was injected into the chromatographic column of high-speed countercurrent chromatography at a flow rate of 20ml / min. Turn on the countercurrent chromatograph to 800 revs / min, then pump the sample solution (15ml lower phase solution with 0.6g mulberry anthocyanin crude ...

Embodiment 3

[0047] The solvent system of the present embodiment adopts TBME by volume ratio: acetonitrile: n-butanol: TFA aqueous solution=1: 1: 3.5: 6, the concentration of TFA aqueous solution is to contain 5 milliliters of TFA in every liter of aqueous solution, countercurrent chromatograph is HSCCC-D1000 High-speed countercurrent chromatography, the content of anthocyanins in the crude extract of mulberry anthocyanins accounts for about 38%.

[0048] Measure 250ml of TBME, 250ml of acetonitrile, 875ml of n-butanol and 1500ml of TFA aqueous solution, put it in a 3000ml separatory funnel, shake it well, after standing for stratification, put the upper and lower phases into reagent bottles respectively. The upper phase was injected into the chromatographic column of high-speed countercurrent chromatography at a flow rate of 40ml / min. Turn on the countercurrent chromatograph to 800 revs / min, then pump the sample solution (40ml lower phase solution with 1.5g mulberry anthocyanin crude extr...

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Abstract

This invention relates to a method of prepare high-purity anthocyanin monomer from mulberry. It adopts ethanol to leach anthocyanin in mulberry; use cation exchange resin to carry out carry out initiative purification to mulberry ethanol; use counter-current chromatography technology to separate anthocyanin in mulberry anthocyanin crude extract, the solvent system composed by n-butanol, acetonitrile, mtbe ( TBME) and trifluoroacetic acid ( TFA) water solution. The volume ratio of TBME, acetonitrile and n-butanol is 1: 0.5 to 1: 1.25 to 3.5. The concentration of TFA water solution is 1 to 10 milliliter TFA in per liter water solution. This invention takes high speed counter-current chromatography instrument ( HSCCC) or low speed counter-current chromatography instrument ( SRCCC) as separation plant, effectively reduce preparation cost of anthocyanin monomer.

Description

technical field [0001] The invention belongs to the technical field of food processing and relates to the deep processing technology of mulberries, in particular to a method for separating and preparing high-purity anthocyanin monomers from mulberries. technical background [0002] Mulberry is rich in nutrients and anthocyanins, and also has high medicinal value. The Food Sanitation Supervision and Inspection Institute of the Ministry of Health of my country has included mulberry in the list of both food and medicine in 1988. Existing studies have shown that natural anthocyanins have strong antioxidant activity, can eliminate excessive free radicals produced during the body's metabolism, delay aging, prevent various diseases, etc., and also have biological activities such as anti-cancer and anti-mutation , and have a protective effect on DNA splitting and reduce eye fatigue, improve night vision and improve the adaptability of visual instant changes. [0003] The existing s...

Claims

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Application Information

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IPC IPC(8): C07H17/06C07H1/08A61K36/605A61K131/00
Inventor 杜琪珍
Owner ZHEJIANG GONGSHANG UNIVERSITY
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