Pretreatment method for liquid-phase micro-extraction sample

Abstract

This invention relates to one liquid phase extract sample front process method, which comprises the following steps: a, taking one magnetic mixture device and one tube cover PCR tube for 0.2 ml without cover, one sample bottle, bottle plug and mixture magnetic probe; b, containing extraction liquid in bottom of PCR tube for liquid sealing by sample liquid; c, plugging the PCR tube into bottle plug; d, taking sample liquid in the sample bottle with mixture magnetic probes; e, containing the plug of tube into sample bottle for mixing and extraction.

Description

technical field [0001] The invention relates to a liquid phase micro-extraction sample pretreatment method. Background technique [0002] The sample pretreatment technology has an important impact on the analysis results and has been paid much attention. Currently widely used pretreatment techniques include liquid-liquid extraction (LLE), solid-phase extraction (SPE), solid-phase microextraction (SPME), and membrane extraction. Classical liquid-liquid extraction is cumbersome to operate, uses a large amount of organic solvents that are toxic or harmful to humans and the environment, and is not easy to automate. SPE technology has been rapidly developed in many fields due to its advantages of high efficiency, reliability, and low solvent consumption, but it needs to elute and purify substances to meet the requirements of instrumental analysis such as gas chromatography (GC) or high performance liquid chromatography (HPLC). SPME is a solvent-free sample pretreatment technolo...

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Problems solved by technology

[0006] (2) The liquid droplets suspended on the needle tip will drop due to slight vibrations, so the experiment has very high requirements on the experimental environment, and requires the operator to be extremely careful, and the stirring speed of the sample pool must also be adjusted very low

[0007] (3) Due to the decrease of the droplet volume, its vapor pressure rises, causing the extraction agent to be extremely volatile, and the extraction time is limited. Generally speaking, the extraction of the analyte has stopped before the equilibrium between the two phases is reached, and the equilibrium cannot be achieved. Really realized, resulting in relatively poor enrichment effect and relatively low sensitivity

Since the liquid film is always fixed on the microporous film, the choice of organic solvent in the liquid film is very important to its stability, and must meet the conditions such as non-water solubility, non-volatility and low viscosity, so there are only a few organic solvents ( n-undecane, n-hexyl ether, etc.) are available, and the efficiency of using these solvents to separate and enrich polar compounds is often low

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