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Novel use of Bitai-en for protecting liver stem cell, eliminating apoptosis of said cells and protecting nepatic function

A technology of liver stem cells and liver cells, which can be applied to medical preparations containing active ingredients, pill delivery, digestive system, etc., can solve problems that have not been paid attention to, and achieve the effect of improving pathological changes and blood sperm enzymatic indicators

Inactive Publication Date: 2007-07-18
丁先风
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are also people abroad such as Italy (the country's Pharmacopoeia), and the United States is used to treat alcoholic hepatitis and non-specific hepatitis caused by various reasons, liver cirrhosis (Barak, et al.United States Patent, 5,428,063), and belongs to the 1990s. Patents, which have not been taken seriously in China (Chinese patent search and literature search)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1. Effect of Bitaien on Rat Hepatic Stem Cells and Hepatocytes in Culture

[0027]1. Materials Imported premium fetal bovine serum (fetal bovine serum, FBS, Gibco), imported FBS and domestic newborn calf serum (newborn calf serum, NCS; provided by Hangzhou Sijiqing Bioengineering Materials Research Institute). DMEM / F12 (high sugar, no sodium pyruvate, sigma), non-essential amino acids (×100, Gibco), Bitaine (sigma, purity 99.9%). Conalbumin (Conalbumin, Sigma). Fast blue BB salt (Fast blue BB salt, Sigma) and naphthol-AS-MX (Naphthol ASMX phosphate, Sigma).

[0028] Liver stem cell medium: 5% FBS (domestic or imported), 10% domestic NCS, 0.14mM β-ME, 40ng.ml -1 Conalbumin, bovine serum albumin, 1-5 μg / ml leukemia inhibitory factor (LIF), 1% non-essential amino acid high glucose DMEM / F12 medium. 0.2% gelatin: gelatin (type A, G2500, Sigma), prepared with 0.01M PBS, autoclaved for use.

[0029] II. Methods Preparation of rat embryonic liver cells Take mouse...

Embodiment 2

[0045] Embodiment two, than Tyne to CCl in in vitro culture 4 Effects of injured rat hepatocytes

[0046] Objective: To primary culture rat hepatocytes and observe the effect of Bitaine on CCl 4 Protective Effect of Injured Rat Hepatocytes

[0047] 1. Materials and methods 1.1 SD male rats (provided by the Experimental Animal Center of Sun Yat-Sen University School of Medicine), weighing 180-220 g, free to eat and drink.

[0048] 1.2 Main reagents: Bitaine, collagenase (type II), and insulin are all products of Sigma (St.Louis, MO, USA); 1640 medium is a product of Gibco (Grand Island, NY); bovine serum albumin (BSA) It is the product of Hangzhou Sijiqing Company; CCl 4 (analytical pure).

[0049] 1.3 Isolation and primary culture of hepatocytes: Anesthetize the rats with 3% sodium pentobarbital, intubate the portal vein, cut off the inferior vena cava, and quickly perfuse with D-Hanks solution to wash away the residual blood at a flow rate of 30-40ml / min , for about 10 m...

Embodiment 3

[0058] Embodiment three, than Tyne to CCl 4 Effects of Acute Liver Injury on Injured Rats

[0059] CCl 4 After entering the body, it is activated by liver cytochrome P450 to generate trichloromethyl free radicals (CCl 3 ), attack the phospholipid molecules on the endoplasmic reticulum membrane through the adsorption of hydrogen, causing lipid peroxidation of the membrane, CCl 3 Then covalently bind to membrane lipids and protein macromolecules, causing damage to membrane structure and functional integrity, CCl 3 It can also inhibit the activity of calcium ion pump on the cell membrane and microsomal membrane, so that Ca 2+ Increased influx of ions leads to toxic cell death. This example utilizes CCl 4 Make a mouse model of liver injury, and observe the protective effect of Bitaine on the liver injury of mice.

[0060] 1. Materials and methods: 1.1 32 healthy male HIH mice were provided by the Experimental Animal Center of Sun Yat-Sen University School of Medicine, weighi...

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PUM

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Abstract

An application of Bitaien in preparing the medicines in the form of capsule, oral liquid, particle, tablet, or injection for preventing and treating fatty liver, alcoholic liver, hepatitides, rheumatism, osteoporosis, gout, diabetes, cardiovascular and cerebrovascular diseases, etc, preventing cancer, delaying sanility, improving immunity, etc is disclosed.

Description

technical field [0001] The invention relates to the fields of protecting liver stem cells, inhibiting apoptosis of liver stem cells and protecting liver function Background technique [0002] my country is a high-incidence area of ​​viral hepatitis, and about 120 million people carry hepatitis B virus, which is one of the diseases that seriously endanger the health of our people. Hepatitis Trilogy—The final outcome of chronic hepatitis includes recovery, liver fibrosis and cirrhosis, and liver cancer. Hepatic decompensation caused by liver disease has become the main cause of serious threat to patients with liver disease and disability and death. With the continuous improvement of people's living standards and unhealthy diet, the incidences of alcoholic hepatitis, liver fibrosis, liver cirrhosis (alcoholic hepatic scleroses, AHS) and nonalcoholic liver cirrhosis (nonalcoholic steatohepatitis, NASH) are also increasing year by year. Can cause hepatic decompensation. In add...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/205A61K9/08A61K9/10A61K9/16A61K9/20A61K9/48A61P1/16A61P29/00A61P19/10A61P19/06A61P3/10A61P9/10A61P37/02
Inventor 丁先风
Owner 丁先风
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