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Diagnosis chip for testing subtype of human papilomavirus gene and preparation method and test method

A technology of human papillomavirus and genotyping, which is applied in the field of human papillomavirus genotyping detection and diagnosis chip, and can solve the problem of not being able to meet the genotype of HPV virus

Inactive Publication Date: 2008-07-09
ZIBO HIGH TECH TIANJIN CITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the diagnosis of HPV infection mainly relies on the genetic detection of viral nucleotide sequences by molecular biology techniques, including gene sequencing, hybrid capture, dot hybridization, slot hybridization and specific probes, etc. have been applied to HPV research, but Due to their respective limitations and special requirements, it is impossible to obtain the genotype of HPV virus infected by patients in a short time and high throughput in clinical testing

Method used

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  • Diagnosis chip for testing subtype of human papilomavirus gene and preparation method and test method
  • Diagnosis chip for testing subtype of human papilomavirus gene and preparation method and test method
  • Diagnosis chip for testing subtype of human papilomavirus gene and preparation method and test method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Probes are set on the chip substrate, including 1 universal probe for identifying all HPV genotypes and 15 HPV genotype-specific probes for distinguishing HPV 6, HPV11, HPV16, HPV18, HPV31, HPV33, HPV35, HPV39, HPV42 , HPV43, HPV44, HPV45, HPV52, HPV56, and HPV58, a total of 15 genotypes, the probes are arranged in a microarray on the chip, and the rectangular spotting area of ​​the chip is controlled by 5 coordinate points, and the rectangular spotting area of ​​the chip is controlled by coordinate points and Spotting direction, set 2 coordinate points in one corner of the microarray, which is the starting point of spotting, indicating the arrangement direction of the probes, and 1 coordinate point in the other 3 corners of the microarray, and the vertical column of the microarray is 4 columns, The horizontal row of the microarray is 7 rows. Each HPV35, HPV42, HPV43, HPV45, HPV56, HPV58 probe point is 1, general probe, HPV6, HPV11, HPV16, HPV18, HPV31, HPV33, HPV44, HP...

Embodiment 2

[0054] Probes are set on the chip substrate, including 1 universal probe for identifying all HPV genotypes and 15 HPV genotype-specific probes for distinguishing HPV 6, HPV11, HPV16, HPV18, HPV31, HPV33, HPV35, HPV39, HPV42 , HPV43, HPV44, HPV45, HPV52, HPV56, and HPV58, a total of 15 genotypes, the probes are arranged in a microarray on the chip, and the rectangular spotting area of ​​the chip is controlled by 5 coordinate points, and the rectangular spotting area of ​​the chip is controlled by coordinate points and Spotting direction, set 2 coordinate points in one corner of the microarray, which is the starting point of spotting, indicating the arrangement direction of the probes, and 1 coordinate point in the other 3 corners of the microarray, and the column of the microarray is 3 columns, The horizontal row of the microarray is 9 rows. Each HPV35, HPV42, HPV43, HPV45, HPV56, HPV58 probe point is 1, general probe, HPV6, HPV11, HPV16, HPV18, HPV31, HPV33, HPV44, HPV52 probe...

Embodiment 3

[0056] Probes are set on the chip substrate, including 1 universal probe for identifying all HPV genotypes and 15 HPV genotype-specific probes for distinguishing HPV 6, HPV11, HPV16, HPV18, HPV31, HPV33, HPV35, HPV39, HPV42 , HPV43, HPV44, HPV45, HPV52, HPV56, and HPV58, a total of 15 genotypes, the probes are arranged in a microarray on the chip, and the rectangular spotting area of ​​the chip is controlled by 5 coordinate points, and the rectangular spotting area of ​​the chip is controlled by coordinate points and Spotting direction, set 2 coordinate points in one corner of the microarray, which is the starting point of spotting, indicating the arrangement direction of the probes, and 1 coordinate point in the other 3 corners of the microarray, and the column of the microarray is 6 columns, The horizontal row of the microarray is 5 rows. Each HPV35, HPV42, HPV43, HPV45, HPV56, HPV58 probe point is 1, general probe, HPV6, HPV11, HPV16, HPV18, HPV31, HPV33, HPV44, HPV52 probe...

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Abstract

This invention is a method of preparing and detecting diagnostic chip of human papillomavirus gene. On the base of the chip there are 16 kinds of stylet which arrange in array, they consist of one general stylet, 15stylet subjected to HPV. There are 6, 11, 16, 18, 31, 33, 35, 39, 42, 43, 44, 45, 52, 56, 58 and so on 15 in total. We add a nucleic acid to the end of the atyle, irradiate the chip under the ultraviolet radiation for 5minute, fix the aryle to the surface of the chip, and construct to be diagnostic chip. PCR primer adapts 2-24 chain of primer. This invention has the virtue of quick, exactness, simple and convenient, special recognition, high information, it is prone to expand, and has high economic and social efficiency on clinic application.

Description

technical field [0001] The invention relates to a microbe detection medical in vitro diagnostic technology, in particular to a human papillomavirus genotyping detection and diagnosis chip and a manufacturing method and a detection method. Background technique [0002] The typing research of human papilloma virus (Human papilloma virus, hereinafter referred to as HPV) has always been highly valued by scholars at home and abroad. The confirmation of HPV types in specimens depends on the method used. At present, the diagnosis of HPV infection mainly relies on the genetic detection of viral nucleotide sequences by molecular biology techniques, including gene sequencing, hybrid capture, dot hybridization, slot hybridization and specific probes, etc. have been applied to HPV research, but Due to their respective limitations and special requirements, it is impossible to obtain the genotype of HPV virus infected by patients in a short time and high throughput in clinical testing. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 高英堂方淑昌杜智
Owner ZIBO HIGH TECH TIANJIN CITY
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