Noninvasive polygenic hereditary colorectal cancer detection kit based on next-generation sequencing technology

Abstract

The invention discloses a noninvasive polygenic hereditary colorectal cancer detection kit based on the next-generation sequencing technology. The noninvasive polygenic hereditary colorectal cancer detection kit based on the next-generation sequencing technology comprises primers of SEQ NO:1-SEQ NO:272. The detection kit is capable of detecting 245 highly-susceptible SNP loci of 10 carcinoma-associated genes, has the advantages of high defection flux on carcinoma-associated vulnerable SNP loci, high specificity, low possibility of pollution and high safety, is high in accuracy and repeatability in detection results, and takes certain auxiliary diagnosis and prompt effects on the crowds highly susceptible to the colorectal cancer, especially the crowds with the colorectal cancer family heredity history.

Description

technical field [0001] The invention relates to the field of gene detection, in particular to a kit for non-invasive detection of high-incidence hereditary intestinal cancer susceptibility genes. Background technique [0002] In recent years, the incidence of malignant tumors in my country has shown an obvious upward trend, an increase of 83.1% compared with the mid-1970s. Statistics show that the average cancer rate in my country is 286 per 100,000 people, and the number of deaths reaches 181 per 100,000 people, which means that 2.35 million new cancer cases are diagnosed in my country every year, and 6 people are diagnosed every minute. for cancer. Studies have shown that the formation of cancer is related to multiple factors such as the external environment, living habits, and heredity, among which genetic factors account for about 10% of the cancer population. Hereditary cancers are mainly divided into two categories. One is to pass cancer to the next generation. The co...

AI Technical Summary

Problems solved by technology

[0006]At present, most of the diagnostic methods for hereditary tumors on the market stay at the detection level of single or several sites of a single tumor, and most of the detection methods are fluorescent PCR and chip method These methods have different defects, such as cumbersome operation, complex primer or probe design, difficult interpretation of results, poor repeatability, many false positives and false negatives, less number of combined genes, and low throughput, which are difficult to meet At present, the number of test samples is large, the detection sites are many, the distribution is wide, and the detection accuracy is high. Therefore, a new detection method is urgently needed to meet the needs of the market and medical treatment.

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