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Diagnosis chip for testing subtype of human papilomavirus gene and preparation method and test method

A human papillomavirus and genotyping technology, which is applied in the field of human papillomavirus genotyping detection and diagnosis chips, and can solve problems such as the inability to meet HPV virus genotypes

Inactive Publication Date: 2005-10-26
ZIBO HIGH TECH TIANJIN CITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the diagnosis of HPV infection mainly relies on the genetic detection of viral nucleotide sequences by molecular biology techniques, including gene sequencing, hybrid capture, dot hybridization, slot hybridization and specific probes, etc. have been applied to HPV research, but Due to their respective limitations and special requirements, it is impossible to obtain the genotype of HPV virus infected by patients in a short time and high throughput in clinical testing

Method used

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  • Diagnosis chip for testing subtype of human papilomavirus gene and preparation method and test method
  • Diagnosis chip for testing subtype of human papilomavirus gene and preparation method and test method
  • Diagnosis chip for testing subtype of human papilomavirus gene and preparation method and test method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Probes are set on the chip substrate, including 1 universal probe for identifying all HPV genotypes and 15 HPV genotype-specific probes for distinguishing HPV6, HPV11, HPV16, HPV18, HPV31, HPV33, HPV35, HPV39, HPV42, HPV43, HPV44, HPV45, HPV52, HPV56, and HPV58 have a total of 15 genotypes. The probes are arranged in a microarray on the chip, and the rectangular spotting area of ​​the chip is controlled by 5 coordinate points. Sample direction, set 2 coordinate points in one corner of the microarray, which is the starting point of sample application, indicating the arrangement direction of the probes, and 1 coordinate point in the other 3 corners of the microarray, the column of the microarray is 4 columns, and the microarray The horizontal row of the array is 7 rows. Each HPV35, HPV42, HPV43, HPV45, HPV56, HPV58 probe point is 1, general probe, HPV6, HPV11, HPV16, HPV18, HPV31, HPV33, HPV44, HPV52 probe point is 2, general probe is Consensus sequence of all HPV, HPV6,...

Embodiment 2

[0048] Probes are set on the chip substrate, including 1 universal probe for identifying all HPV genotypes and 15 HPV genotype-specific probes for distinguishing HPV6, HPV11, HPV16, HPV18, HPV31, HPV33, HPV35, HPV39, HPV42, HPV43, HPV44, HPV45, HPV52, HPV56, and HPV58 have a total of 15 genotypes. The probes are arranged in a microarray on the chip, and the rectangular spotting area of ​​the chip is controlled by 5 coordinate points. In the direction of sampling, two coordinate points are set in one corner of the microarray as the starting point of sample application, indicating the arrangement direction of the probes, and one coordinate point is set in the other three corners of the microarray, and the columns of the microarray are 3 columns. The horizontal row of the array is 9 rows. Each HPV35, HPV42, HPV43, HPV45, HPV56, HPV58 probe point is 1, general probe, HPV6, HPV11, HPV16, HPV18, HPV31, HPV33, HPV44, HPV52 probe point is 2, general probe is Consensus sequence of all...

Embodiment 3

[0050] Probes are set on the chip substrate, including 1 universal probe for identifying all HPV genotypes and 15 HPV genotype-specific probes for distinguishing HPV6, HPV11, HPV16, HPV18, HPV31, HPV33, HPV35, HPV39, HPV42, HPV43, HPV44, HPV45, HPV52, HPV56, and HPV58 have a total of 15 genotypes. The probes are arranged in a microarray on the chip, and the rectangular spotting area of ​​the chip is controlled by 5 coordinate points. Sample direction, set 2 coordinate points in one corner of the microarray, which is the starting point of sample application, indicating the direction of probe arrangement, and 1 coordinate point in the other 3 corners of the microarray, the column of the microarray is 6 columns, and the microarray The horizontal row of the array is 5 rows. Each HPV35, HPPV42, HPV43, HPV45, HPV56, HPV58 probe point is 1, general probe, HPV6, HPV11, HPV16, HPV18, HPV31, HPV33, HPV44, HPV52 probe point is 2, general probe is Consensus sequence of all HPV, HPV6, HPV...

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Abstract

This invention is a method of preparing and detecting diagnostic chip of human papillomavirus gene. On the base of the chip there are 16 kinds of stylet which arrange in array, they consist of one general stylet, 15stylet subjected to HPV. There are 6, 11, 16, 18, 31, 33, 35, 39, 42, 43, 44, 45, 52, 56, 58 and so on 15 in total. We add a nucleic acid to the end of the atyle, irradiate the chip under the ultraviolet radiation for 5minute, fix the aryle to the surface of the chip, and construct to be diagnostic chip. PCR primer adapts 2-24 chain of primer. This invention has the virtue of quick, exactness, simple and convenient, special recognition, high information, it is prone to expand, and has high economic and social efficiency on clinic application.

Description

technical field [0001] The invention relates to a microbe detection medical in vitro diagnostic technology, in particular to a human papillomavirus genotyping detection and diagnosis chip and a manufacturing method and a detection method. Background technique [0002] The typing research of human papilloma virus (Human papilloma virus, hereinafter referred to as HPV) has always been highly valued by scholars at home and abroad. The confirmation of HPV types in specimens depends on the method used. At present, the diagnosis of HPV infection mainly relies on the genetic detection of viral nucleotide sequences by molecular biology techniques, including gene sequencing, hybrid capture, dot hybridization, slot hybridization and specific probes, etc. have been applied to HPV research, but Due to their respective limitations and special requirements, it is impossible to obtain the genotype of HPV virus infected by patients in a short time and high throughput in clinical testing. ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 高英堂方淑昌杜智
Owner ZIBO HIGH TECH TIANJIN CITY
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