Bovine serum albumin hot ethanol extracting process

Abstract

The invention discloses a process for extracting bovine serum albumin through thermal alcohol method comprising the steps of, separating plasma, charging sodium citrate into ox blood, centrifugal extracting, placing the collected plasma into laminated reaction tank, heating and charging in sodium octoate, agitating slowly, the temperature being 55-65 deg. C, adjusting pH to 4.0-6.5 by charging in hydrochloric acid, charging in alcohol, filtering, desalinizing and concentrating, freeze-drying to obtain the end product. The process requires less material by short process flow, it is suitable fo mass production.

Description

technical field [0001] The invention relates to a method for extracting albumin from bovine serum, in particular to a hot ethanol extraction process for bovine serum albumin. Background technique [0002] Bovine serum albumin is used for the coating of diagnostic reagents. There are many preparation methods, but most of them are still in the experimental stage. The following methods are suitable for pilot test and formal production: (1) Salting out method, cold salting out method And the hot salting out method, the main technological process of the cold salting out method is to centrifuge fresh bovine blood, add pure water, add ammonium sulfate, filter, filtrate dialysis desalination, add polyethylene glycol, dialysate concentration, freeze-drying to be the finished product; This method uses a lot of materials, adding ammonium sulfate requires 40-50% of the amount of cattle blood, adding polyethylene glycol requires 2-4 times the amount of cattle blood, and adding pure water...

AI Technical Summary

Problems solved by technology

[0002] Bovine serum albumin is used for the coating of diagnostic reagents. There are many preparation methods, but most of them are still in the experimental stage. The following methods are suitable for pilot test and formal production: (1) Salting out method, cold salting out method And the hot salting out method, the main technological process of the cold salting out method is to centrifuge fresh bovine blood, add pure water, add ammonium sulfate, filter, filtrate dialysis desalination, add polyethylene glycol, dialysate concentration, freeze-drying to be the finished product; This method uses a lot of materials, adding ammonium sulfate requires 40-50% of the amount of cattle blood, adding polyethylene glycol requires 2-4 times the amount of cattle blood, and adding pure water for dialysis requires 20-40 times the amount of cattle blood; Time-consuming, the cold salting method requires 48 hours for dialysis, 24 hours for dehydration, and 5 working days for the whole process; the yield is low, generally 0.8-1.0%; the above three points make the production efficiency of the cold salting method low and the cost higher

(2) Organic solvent precipitation method, the E.J.Cohn method is the most commonly used method, but this method needs to be carried out at a low temperature of 0°C to 5°C, which is time-consuming and laborious. It takes 7 days for a single pass, and the yield is not high, about 1%, and its cost is also high.

Images