The invention discloses a disposable hemodialysis nursing kit, which comprises a box body and a box cover. The inside of the box body is divided into an on-machine treatment and nursing area for hemodialysis catheters, an off-machine treatment and nursing area for hemodialysis catheters, and an internal fistula through partitions. In the puncture nursing area, a disposable bed patch bag, three rubber gloves, a piece of non-woven treatment towel, a piece of iodophor gauze, a piece of alcohol gauze and four alcohol cotton balls, two A pair of 5ml syringes, a piece of sterile gauze, a piece of sterile applicator, a piece of adhesive tape, two disposable treatment trays, and an outer packaging bag. The items except the packaging bag are stacked in the outer packaging bag from top to bottom Inside. The invention has simple structure, very fast and convenient use, can ensure the aseptic state in the operation process, and greatly reduces the chance of cross-infection.