30 results about "Blatella germanica" patented technology

The invention provides a novel pyrethroid compound having the following structural formula (X), a preparation method of the compound and application thereof in sanitary desinsection products. In the formula, R is methoxymethyl (-CH2OCH3) or an H atom. Experiments show that the compound of the formula (X) has very good control efficiency on sanitary insect pests such as mosquito, fly, and blattella germanica, etc. In addition, the advantage which is especially worth being noticed is that the single optical isomer of the dextral cis form of the compound has better desinsection activity compared with corresponding despinner.

The invention provides a novel pyrethroid compound with the following structural formula (X), a preparation method thereof and application of the compound in health disinsection products. The R in the formula is methoxymethyl (-CH2OCH3) or H atom. Tests show that the compound shown in the formula (X) has excellent control effect against pests such as mosquitoes, flies and Blattella germanica; and particularly, the dextral trans-form single optical isomer of the compound has better disinsection activity compared with corresponding racemate.

The invention discloses an insecticidal composition taking fermentation liquid containing metarhizium anisopliae and hydramethylnon as main active ingredients. The composition takes metarhizium anisopliae powder, metarhizium anisopliae fermentation liquid and the hydramethylnon as the main active ingredients; or the composition takes the fermentation liquid containing the metarhizium anisopliae and the hydramethylnon as the main active ingredients. The results of an indoor toxicity test show that the insecticide has a good killing effect on blattella germanica and has a significant synergisticeffect.

The invention relates to a multi-PCR primer and a method for quickly identifying a blattaria insect variety, belonging to the technical field of applied biology. The multi-PCR primer for quickly identifying the blattaria insect variety comprise a periplaneta americana primer, a periplaneta fuliginosa primer, a periplaneta australasiae primer, a periplaneta brunnea primer, a blattella germanica primer and a ground beetle primer. The identifying method comprises the following steps: extracting blattaria insect genome total DNA, performing multi-PCR amplification, and judging a variety of a blattaria insect to be detected according to PCR product size obtained by sepharose gel electrophoresis detection. PCR product strip sizes of periplaneta americana, periplaneta fuliginosa, periplaneta australasiae, periplaneta brunnea, blattella germanica and ground beetle are respectively 452bp, 125bp, 160bp, 284bp, 253bp and 380bp. The identifying primer and the multi-PCR method can be used for sensitively and quickly determining the variety of the blattaria insect sample to be detected, and the method has the advantages of simple operation, high repeatability, low cost and the like in comparisonto a traditional detection method.

The invention relates to a composition containing etrafluorine ethofenprox and high-efficiency cypermethrin and application thereof, and provides a pesticidal composition for controlling health mosquitoes, health flies, Blattella germanica and other health pests, wherein the mass ratio of the tetrafluorine ethofenprox to the high-efficiency cypermethrin is 1:99-99:1. The contained two pesticides have the advantages of synergy during compounding and capacity of improving the effect of controlling the health insects; and products such as mosquito coils, insecticide aerosols prepared from the pesticidal composition have the advantages of rapidness for killing the pests and durable pesticide effect, and can ideally control the health pests with resistance to pyrethroid pesticides.

A German cockroach trap provides bait, humidity, and temperature to attract German cockroaches into entries, electrocutes the German cockroaches upon entry into the trap, and stores dead cockroaches below the entries. Electrode bands form a closed ring on an interior surface and contact with two of the rings immediately kills the German cockroach which drops into a receiving dock below. A center electrode band may include holes for entry into the trap. A water reservoir in the bottom of the trap is heated to generate humid vapor. Heaters in the top of the trap are controlled to maintain temperature and a fan in the top of the trap draws ambient air into the trap to create a humid vapor flow out through the entries. Bait trays contain material further attracting the German cockroaches.

The invention relates to a tetraflumethrin and deltamethrin-containing composition and application thereof, and provides a pesticide composition for preventing and controlling sanitary pests, such as sanitary mosquitoes, sanitary flies, Blattella germanica and the like. The mass ratio of the tetraflumethrin to the deltamethrin is 1:99-99:1. The two pesticides contained in the composition show obvious synergistic effect when compounded, so the sanitary pest prevention and control effect can be obviously improved; and the pesticide composition has the advantages of quick killing of pests and enduring pesticide effect when used for preparing the products, such as disk-type mosquito coils, insect aerosol and the like, and can very ideally prevent and control the sanitary pests which have resistance against dimethrin pesticides.

The invention discloses a gene, a gene fragment, a corresponding dsRNA that regulates the synthesis of sex pheromone of Blattella germanica and a preparation method and application thereof. The nucleotide sequence of the gene that regulates the synthesis of sex pheromone of Blattella germanica is as shown in SEQ ID No.1. The nucleotide sequence of the gene fragment is as shown in SEQ ID No.3. Introduction of dsRNA synthesized using the fragment as a template into female Blattella germanica can significantly inhibit the expression level of CYP4PC1 gene, leading to the reduction of methyl ketonesex pheromone synthesis. Finally, the courtship behavior of some male individuals of Blattella germanica disappears. The invention provides a potential molecular target for the management strategy based on cockroach mating and reproduction.

The invention relates to a cyhalothrin and bendiocarb-containing composition and application thereof and provides a pesticidal composition for controlling health mosquitoes, health flies, blattella germanica, solenopsis invicta buren and other health pests, wherein the mass ratio of the cyhalothrin to the bendiocarb is 1:99-99:1. The contained two pesticides have the advantages of different toxicology mechanisms, independent function, synergy during compounding, and capacity of improving the effect of controlling the health pests; the composition can control each metamorphosis stage and developmental stage of the health pests in the whole life history due to related coaction of the pesticides in the pest bodies; and products such as mosquito coils, insecticide aerosol, insecticide electrical mosquito mats, insecticide baits prepared from the pesticidal composition have the advantages of rapidness for killing the pests and durable pesticide effect, and can ideally control the health pests with resistance to pyrethroid pesticides.

The invention provides a biological source insecticidal composition and applications thereof. According to the invention, Metarhizium spores and boric acid are used as main active components, and auxiliary agents such as trehalose, sorbitol and the like are added, such that the moisture evaporation loss is reduced, the activity of Metarhizium spores is improved, and the use period is prolonged; and creamy flavors and Blattella germanica exoskeleton powder rich in cockroach pheromone are adopted to induce cockroaches to eat, and extra sugar is not introduced as a phagostimulant, such that the possibility of mixed bacterial introducing is reduced while the induction range is expanded and the attraction effect is enhanced so as to substantially prolong the storage and use period and achieve good practical application value.

The invention relates to a composition containing tetraflumethrin and cypermethrin and application thereof and provides a pesticidal composition for preventing and controlling sanitary pests such as sanitary mosquitoes, sanitary flies, blattella germanica and the like. The mass ratio of the tetraflumethrin to the cypermethrin is 1:99-99:1. When compounded, two pesticides contained in the composition have remarkable mutual synergistic effect and can remarkably improving the prevention and control effect on the sanitary pests. When used for preparing products such as coil-type insect-killing mosquito incenses, pesticidal aerosols and the like, the pesticidal composition has the advantages of rapid killing of the pests and durable pesticide effect and can ideally prevent and control the sanitary pests which are resistant to dimethrin medicaments.

The invention discloses a blattella germanica sodium-ion channel resistance mutant gene. The DNA sequences of the mutant gene are shown as SEQ ID NO.1. A protein, an amplification primer, an identification primer and an identification method are also disclosed. A pair of primers can be used to amplify the gene fragments of related resistance gene mutation of blattella germanica, and sequencing canbe performed; and blattella germanica resistance genes can be identified through a four-primer ARMS-PCR method. The mutant gene can be widely applied to the identification demands of blattella germanica resistance gene mutation and corresponding scientific research fields.

The invention discloses a method for producing German cockroach allergen Bla g 7 protein in a rhabdovirus-insect expression system, comprising the following steps: total RNA is extracted from the tissue of the German cockroach, the total RNA is reversely transcripted into cDNA, a coding gene of German cockroach allergen Bla g 7 is obtained by the method of PCR; the coding gene is cloned to a rhabdovirus carrier, insect cells are transformed for expression; the method of affinity chromatography is adopted for purification, thus obtaining the German cockroach allergen Bla g 7 protein. The protein is different from Bla g 7 protein of foreign sources in terms of 2 amino acid locuses. In the invention, the rhabdovirus-insect expression system is adopted to produce German cockroach allergen Blag 7 protein, thus avoiding influence on Bla g 7 allergen structures in different expression systems (such as bacterial and microzyme); in addition, the invention provides the method of affinity chromatography, thus solving the problem that the Bla g 7 allergen has complex purification process.

The invention discloses a blattella germanica knockdown resistance-related mutant gene. A DNA sequence thereof is shown in SEQ ID NO. 1. A protein thereof and an identification primer and an identification method are disclosed. The method amplifies a mutant gene fragment of the blattella germanica knockdown resistance-related gene by a pair of primers and performs sequencing, and identifies a blattella germanica resistance gene by a four-primer ARMS-PCR method. The method can be widely applied to the identification requirements of blattella germanica resistance gene mutation and correspondingscientific research fields.

The invention provides an application of paralytic shellfish poisoning (PSP) toxin to preparation of a medicament for controlling Blattella germanica, provides an application of paralytic shellfish poisoning toxin homologue GC 1-3 to preparation of a medicament for controlling Blattella germanica, and provides a composition for killing Blattella germanica which contains paralytic shellfish poisoning toxin homologue GC 1-3 as a poisoning agent as well as one or several kinds of auxiliary materials with food calling effects and others.

The invention relates to a multi-PCR primer and a method for quickly identifying a blattaria insect variety, belonging to the technical field of applied biology. The multi-PCR primer for quickly identifying the blattaria insect variety comprise a periplaneta americana primer, a periplaneta fuliginosa primer, a periplaneta australasiae primer, a periplaneta brunnea primer, a blattella germanica primer and a ground beetle primer. The identifying method comprises the following steps: extracting blattaria insect genome total DNA, performing multi-PCR amplification, and judging a variety of a blattaria insect to be detected according to PCR product size obtained by sepharose gel electrophoresis detection. PCR product strip sizes of periplaneta americana, periplaneta fuliginosa, periplaneta australasiae, periplaneta brunnea, blattella germanica and ground beetle are respectively 452bp, 125bp, 160bp, 284bp, 253bp and 380bp. The identifying primer and the multi-PCR method can be used for sensitively and quickly determining the variety of the blattaria insect sample to be detected, and the method has the advantages of simple operation, high repeatability, low cost and the like in comparisonto a traditional detection method.

The invention discloses a method for producing German cockroach allergen BgGSTD1 protein in rhabdovirus-insect expression system, comprising the following steps: total RNA is extracted from the tissue of the German cockroach, the total RNA is reversely transcripted into cDNA, a coding gene of German cockroach allergen BgGSTD1 is obtained by the method of PCR; the coding gene is cloned to a rhabdovirus carrier, insect cells are transformed for expression; the method of affinity chromatography is adopted for purification, thus obtaining the German cockroach allergen BgGSTD1 protein. In the invention, the rhabdovirus-insect expression line is adopted to produce the German cockroach allergen BgGSTD1 protein, thus avoiding influence on Bla g 7 allergen structures in different expression systems (such as bacterial and microzyme); in addition, the invention provides the method of affinity chromatography, thus solving the problem that the BgGSTD1 allergen has complex purification process.

The invention discloses a method for producing German cockroach allergen Bla g 7 protein in a rhabdovirus-insect expression system, comprising the following steps: total RNA is extracted from the tissue of the German cockroach, the total RNA is reversely transcripted into cDNA, a coding gene of German cockroach allergen Bla g 7 is obtained by the method of PCR; the coding gene is cloned to a rhabdovirus carrier, insect cells are transformed for expression; the method of affinity chromatography is adopted for purification, thus obtaining the German cockroach allergen Bla g 7 protein. The protein is different from Bla g 7 protein of foreign sources in terms of 2 amino acid locuses. In the invention, the rhabdovirus-insect expression system is adopted to produce German cockroach allergen Bla g 7 protein, thus avoiding influence on Bla g 7 allergen structures in different expression systems (such as bacterial and microzyme); in addition, the invention provides the method of affinity chromatography, thus solving the problem that the Bla g 7 allergen has complex purification process.

4-Hydroxy-6-methyl-3-(3-methylbutyryl)-2-pyrone can quickly and effectively control arthropods such as Dictoptera insects (eg German cockroach, Periplaneta smoke).

The invention provides a biological source insecticidal composition and applications thereof. According to the invention, Metarhizium spores and boric acid are used as main active components, and auxiliary agents such as trehalose, sorbitol and the like are added, such that the moisture evaporation loss is reduced, the activity of Metarhizium spores is improved, and the use period is prolonged; and creamy flavors and Blattella germanica exoskeleton powder rich in cockroach pheromone are adopted to induce cockroaches to eat, and extra sugar is not introduced as a phagostimulant, such that the possibility of mixed bacterial introducing is reduced while the induction range is expanded and the attraction effect is enhanced so as to substantially prolong the storage and use period and achieve good practical application value.

The invention discloses an insecticidal composition taking fermentation liquid containing metarhizium anisopliae and hydramethylnon as main active ingredients. The composition takes metarhizium anisopliae powder, metarhizium anisopliae fermentation liquid and the hydramethylnon as the main active ingredients; or the composition takes the fermentation liquid containing the metarhizium anisopliae and the hydramethylnon as the main active ingredients. The results of an indoor toxicity test show that the insecticide has a good killing effect on blattella germanica and has a significant synergisticeffect.

The invention discloses a Blattella germanica kdr mutant allele detection kit and special primers thereof. The invention provides primers for detecting kdr gene mutant site, which are composed of a primer set A and a primer set B, wherein the primer set A comprises a primer 2 and a primer 3, and the primer set B comprises a primer 2 and a primer 4. The experiment proves that the invention provides a quick, simple and sensitive kit for detecting Blattella germanica drug resistance in order to master the drug resistance of Blattella germanica to pyrethroid insecticides.

A method for use of ingestible boric acid as an insect-killing chemical to kill Blatella germanica is disclosed, said boric acid being separate from and juxtaposed to honey made non-ingestible by absorption into wood, the presence of said non-ingestible honey stimulating feeding behavior by said Blatella germanica, which ingest the boric acid as they might ingest a food.

The invention provides an insecticidal composition for controlling the sanitary insect pests such as mosquitoes, flies, blattella germanica, and the like. The insecticidal composition comprises a component (A) dichlor dimefluthrin and a component (B) 2, 3, 5, 6-tetrafluoro-4-methoxyl methyl benzyl-3-(3, 3, 3-trifluoro-propenyl)-2, 2-dimethyl propane carboxylate, wherein, the mass ratio between the component (A) and the component (B) is 1:20 to 99:1. The coil type mosquito repellent incenses, the electric mosquito-repellent mats, the electric mosquito repellent vaporizer, the insecticide aerosol and other products which are prepared by using the insecticidal composition have the advantages of rapid killing to the insect pests and long-term drug effect.