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example 1
ent of Cellulosic Fiber with a Combination of Liquid Natural Polymer and Enzyme
[0095]Ten pulp samples—equivalent to 24 grams of oven dry bleached eucalyptus kraft fiber each—were prepared by diluting never-dried pulp fiber to 10% w / w consistency with deionized water. The resultant pulp slurries (pH 7.8) were then pre-incubated for 20 minutes at 40° C. After pre-incubation, a liquid composition of liquid natural polymer, LNP derived from waxy corn starch, cellulase A of SEQ ID No: 1 from Novozymes (Bagsvaerd, Denmark) and / or cellulase B of SEQ ID No: 2 from Novozymes (Bagsvaerd, Denmark) were added to the 10 pre-heated aliquots according to table 1.
TABLE 1LNPCellulase ACellulase BSample #Identifier(kg / t)(g / t)(g / t)1Control———2EGA50— 50—3EGA100—100—4EGB50—— 505EGB100——1006LNP5——7LNP + EGA505 50—8LNP + EGA1005100—9LNP + EGB505— 5010LNP + EGB1005—100
[0096]When applicable, formulated liquid natural polymer was added to deliver 5 kg of dry waxy corn starch per metric ton of oven dry bleach...
example 2
er Pretreatment of Cellulosic Fiber with a Combination of Liquid Natural Polymer and Enzyme
[0100]Thirteen pulp slurries were prepared by diluting 24 grams of oven dry equivalents of never-dried bleached eucalyptus kraft fiber to 10% w / w consistency with deionized water. The resultant pulp slurries (pH 7.8) were then pre-incubated separately for 20 minutes at 40° C. After pre-incubation, the pulp slurry was divided into one group of four and three groups of three. The first group of four served as the untreated control group (labelled “Control”). The first group of 3 slurries (labelled “LNP”), were treated with a liquefied waxy corn starch (Ingredion, Westchester, Ill.). The second group of 3 slurries were treated with cellulase A of SEQ ID No: 1 from Novozymes (Bagsvaerd, Denmark) (labelled “Cellulase A”). The third group of 3 slurries was treated with both the Cellulase A and the liquefied waxy corn starch (labelled “LNP+Cellulase A”). In all relevant instances, formulated liquefie...
example 3
ent of Cellulosic Fiber with a Combination of Liquid Natural Polymer and Enzyme
[0106]Once-dried bleached eucalyptus kraft pulp (BEKP) was soaked in deionized water at 10% w / w consistency at room temperature overnight and was repulped at 500 rpm for 5 minutes. The resultant pulp was concentrated by centrifuge, dispersed, and stored at 4° C. before use.
[0107]Four pulp samples—equivalent to 24 grams of oven dry fiber each—were prepared by diluting to 4.0% w / w consistency with deionized water. The resultant pulp slurries (pH 6.8) were then pre-incubated for 30 minutes at 40° C. After pre-incubation, a liquid composition of liquid natural polymer, LNP derived from waxy corn starch, and cellulase A of SEQ ID No: 1 from Novozymes (Bagsvaerd, Denmark) were added to the pre-heated pulp slurries according to Table 2.
TABLE 2LNPCellulase ASample #Identifier(kg / t)(g / t)1BEKP-ctrl——2BEKP-Enz—1503BEKP-LNP7.5—4BEKP-LNP / Enz7.5150
[0108]When applicable, the formulated liquid natural polymer was added t...
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