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Treatment of parasitic infections of fish surfaces

a technology for parasitic infections and fish surfaces, applied in the field of parasitic infection treatment, can solve the problems of high morbidity and mortality in wild and cultured fish, unsolved questions on their residual and toxic effects on the environment, and compounds, etc., and achieve the effect of strong in vitro antiparasitic

Inactive Publication Date: 2020-11-12
NEDERLANDS INST VOOR ECOLOGIE NIOO KNAW
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The fish can be treated with bacteria that produce a special substance called lipopeptide biosurfactant. This can help to make the fish resistance to certain diseases or make them easier to process for consumption.

Problems solved by technology

Diseases in fish, such as freshwater fish caused by parasites in and on their surfaces (gills, skin, fins) are an economic challenge in fish production industry targeting fish for consumption as well as ornamental fish.
One important disease is white spot disease of freshwater fish in particular caused by the parasitic ciliate Ichthyophthirius multifiliis which leads to high morbidity and mortality in both wild and cultured fish, worldwide.
These compounds, however, can have adverse environmental effects.
However, despite the promising reports on antiparasitic effects of natural, semi-synthetic and synthetic plant-derived products, questions on their residual and toxic effects on the environment, fish and humans remain unsolved.
White spot disease causes severe economic losses in fresh water and sea water aquaculture.

Method used

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  • Treatment of parasitic infections of fish surfaces
  • Treatment of parasitic infections of fish surfaces
  • Treatment of parasitic infections of fish surfaces

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0075]In Vitro Effects Pseudomonas fluorescens Strain H6 Lipopeptide Biosurfactant on Ichthyophthirius multifiliis

Tomonts

[0076]Ichthyophthirius multifiliis tomonts were only sensitive to the two highest concentrations (1000 and 100 μg / mL PS), which killed all parasites within 15 min (FIG. 1A).

[0077]Cytoplasmic movements inside the tomonts (FIG. 2A) initially increased when exposed to the lipopeptide biosorfactant, whereafter a disruption of the membrane followed and finally cytoplasm was released into the surroundings of the tomonts (FIG. 2C).

[0078]A 10 μg / mL PS concentration had no effect on this parasite life stage within the observation period tested.

Tomocysts

[0079]Ichthyophthirius multifiliis tomocysts, with their enclosed tomites (FIG. 2B), showed a different sensitivity to the lipopeptide biosurfactant when compared to tomonts (FIG. 1B).

[0080]At the highest tested concentration of 1000 μg / mL PS, the majority of tomites in the tomocysts (83%) were immotile after 15 min of expo...

example 2

Sensitivity of Fish to Pseudomonas Lipopeptide Biosurfactant

[0086]Rainbow trout showed no immediate or late adverse reactions when exposed for 3 h to PS concentrations of 10 and 13 μg / mL. No toxic effects on fish could be detected.

example 3

Comparison of In Vitro Activity of Various Biosurfactants Against Tomonts and Theronts

[0087]Collection of I. multifiliis was performed as described above. Extracts of the biosurfactant massetolide A was obtained from Pseudomonas fluorescens SS101 as described in De Bruijn et al (2008), the putisolvin-like biosurfactant from Pseudomonas putida 267 as described in Kruijt et al (2008) and the viscosin-like biosurfactant of Pseudomonas H6 as described above. A stock solution of 15 mg / mL was prepared for each surfactant by dissolving the product in sterile distilled water whereafter a dilution series was prepared for parasite exposures that was performed as described in above. Concentrations of 0.15 and 0.015 mg / ml were tested for all three biosurfactants against tomonts (one replicate) and theronts (in duplicate) and mortality recorded every 15 min up to 1 h of exposure. Significant differences were calculated for the theront mortality data by analysis of variance followed by Dunnet's p...

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Abstract

The invention relates to the use of bacterial lipopeptide biosurfactants in the treatment of white spot disease in fresh water and marine fish. Particularly useful for treatment of white spot disease are viscosin-like lipopeptide biosurfactants obtainable from the Pseudomonas fluorescens strain H6, massetolide or a derivative thereof and putisolvin or a derivative thereof.

Description

FIELD OF THE INVENTION[0001]The present invention relates to the treatment of parasitic infections causing white spot disease in fish.BACKGROUND OF THE INVENTION[0002]Diseases in fish, such as freshwater fish caused by parasites in and on their surfaces (gills, skin, fins) are an economic challenge in fish production industry targeting fish for consumption as well as ornamental fish. One important disease is white spot disease of freshwater fish in particular caused by the parasitic ciliate Ichthyophthirius multifiliis which leads to high morbidity and mortality in both wild and cultured fish, worldwide.[0003]Several chemicals are currently being applied to control white spot disease in fish, such as in freshwater fish.[0004]Following the ban of the organic dye malachite green, previously used for treatment of the disease, fish farmers apply a number of compounds for disease control including sodium percarbonate, copper sulphate, formalin, peracetic acid and totrazuril. These compou...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K38/12C12R1/39A61P33/02
CPCC12R1/39A61P33/02A61K38/12A61K38/08A61K35/74A01N63/50A01N63/27C12N1/205C12R2001/39
Inventor DE BRUIJN, IRENERAAIJMAKERS, JOSEPHUS MARIABUCHMAN, KURT
Owner NEDERLANDS INST VOOR ECOLOGIE NIOO KNAW
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