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Collagen material and method for producing collagen material

a technology of collagen material and collagen material, which is applied in the direction of macromolecular non-active ingredients, pharmaceutical delivery mechanisms, prosthesis, etc., can solve the problems of not being able to design a collagen material shape and the orientation of the material freely, and not being able to freely set the direction of the material orientation, etc., to achieve the effect of convenient manufacturing

Inactive Publication Date: 2020-03-05
ATREE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0034]The present invention has an advantage effect that it is possible to design a shape and a direction of orientation as to an orientated collagen material freely. Further, the present invention has an advantage effect that it is possible to produce a biocompatible material which an orientation is controlled so that the orientation can be nearly equal to an orientation of each site of a normal tissue of a living organism, and further to put into practice regeneration of normal tissue of a living organism, since it is possible to produce a material having an orientation of collagen according to a site in a tissue from a living organism.
[0035]The present invention has an advantage effect that it is possible to produce a collagen material at a macro size of a millimeter order or more. Moreover, the method of producing a collagen material according to the present invention has an advantage that it is easy to manufacture and it is possible to produce a collagen material capable of being designed their shape freely. Further, the present invention has an advantage effect that if an orientated collagen with a string shape is aligned to a desired direction in a plane shape or a three-dimensional shape, it is possible to design a direction of orientation in the collagen material freely.
[0036]Furthermore, the present invention has an advantage effect that even if a dried orientated or non orientated collagen material is immersed into PBS or cell culture medium etc, or is implanted into the living body, the shape of the material is maintained for necessary period and is not dissolved to the original shape of the collagen gel fragment such as a string shape etc. The reasons why the shape of the material is maintained for necessary period and it is difficult to dissolve, is assumed that according to a triple-strand helix conformation which is one of feature of collagen molecule, a hydrophobic residue is throwed out to outside to make their residues assemble and be firmly fixed.

Problems solved by technology

However, in a method of producing a collagen material as described in the above Patent Literatures 1 and 2, it was impossible to allow a planar shape or a three-dimensional shape such as a ribbon, a sheet, a tube, a block to be freely set, and allow direction of orientation in the material to be freely set.
Moreover, although there is a technique for orientation of a collagen, there are no technique for designing a shape of collagen material and a direction of orientation in the material freely.

Method used

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  • Collagen material and method for producing collagen material
  • Collagen material and method for producing collagen material
  • Collagen material and method for producing collagen material

Examples

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Effect test

example 1

[0094]In a method of the present invention, first of all, a collagen gel was prepared as an orientated collagen (substrate) having a size of a millimeter order or more. As to a collagen gel, 9.3 mg / mL concentration of I type of collagen solution derived from a rat tail (BD Company) was extruded through a nozzle having 0.38 mm of an inside diameter into a plate container containing a phosphate buffered saline (PBS) at 38° C., at pH7.4, and thereby sliding the nozzle to obtain a string like collagen gel having about 1 mm in diameter, about 20 mm of length.

[0095]An orientation of a collagen gel obtained thus was analyzed by a raman spectroscopic microscope (PHOTON Design Corporation). In doing so, an excitation wavelength was set at 514.5 nm using a continuous oscillation argon ion laser Stabilite 2017 (Spectra-Physics, Inc.), a HR-320 (Jovin Yvon S.A.S.) as a spectroscope and a LN / CCD-1100-PB / UV AR / 1 (Roper scientific Inc.) were used respectively. FIG. 5 gives a result of evaluating a...

example 2

[0096]Next, a larger size of the collagen material is tried to obtain. First of all, a orientated collagen gel with a string shape was prepared. As to a collagen gel, 9.3 mg / mL concentration of I type of collagen solution derived from a rat tail (BD Company) was extruded through a nozzle having 0.38 mm of an inside diameter into a plate container containing a phosphate buffered saline (PBS) at 38° C., at pH7.4, and thereby sliding the nozzle to obtain a string like collagen gel having about 1 mm in diameter, about 200 mm of length. FIG. 1 gives a photograph of an orientated collagen gel with a string shape immediately after it is produced in PBS.

[0097]The produced orientated collagen gel with a string shape is aligned on a plane as shown in FIG. 2, and after that is dried to obtain a dried orientated collagen material with a sheet shape.

example 3

[0098]The above mentioned orientated collagen gel with a string is aligned on the plane with a sheet shape. After that, a second layer is aligned wherein a direction of an orientated collagen gel with a string shape is perpendicular to a travel direction of the orientated collagen with a string shape of the first layer (FIG. 3(a)). After that, a dried sample is shown in FIG. 3(b), a sample cut in rectangles after drying is shown in FIG. 3(c). In this way, it is possible to make not only single layer but multiple layers using the orientated collagen sheet with a sheet shape, and further it is possible to freely design a direction of orientation of each layer.

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Abstract

A collagen material is characterized in being constituted of a collagen gel fragment wherein a shape of the collagen gel fragment is a string and the string collagen gel fragments couple with each other by drying, and the collagen gel fragments have orientation obtained by giving a flow of a fixed direction to collagen solution during a process of gelating a collagen solution. A method of producing a collagen material comprises a step for preparing a string collagen gel formed and orientated by collagen solution being extruded through a nozzle into a container containing a phosphate buffered saline, and giving a flow of a fixed direction to the collagen solution wherein a shape of the collagen gel is a string and the direction of the orientation of the string collagen gel is a direction of the string shape, a step for obtaining multiple string collagen gels wherein the orientation are controlled, a step for arranging the multiple collagen gels in a desired shape, and a step for drying the multiple collagen gels arranged in the desired shape to couple with the collagen gels each other.

Description

CROSS REFERENCE TO RELATED APPLICATIONS[0001]The present application is a continuation application of U.S. patent application Ser. No. 14 / 000,397 filed Aug. 19, 2013, which claims priority from PCT Application No. PCT / JP2012 / 001098 filed February 20, 2012 and Japanese Patent Application No. JP 2011-035239 filed Feb. 21, 2011, which are each expressly incorporated by reference herein.Technical Field[0002]The present invention relates to a collagen material, and a method of producing the collagen material, in particular, a collagen material and a method of producing the collagen material, using a collagen segment.Background[0003]In the past, a non orientated collagen was used as a substrate material of a cell culture for many years. On the contrary, a collagen having an orientation is found in tremendous numbers in the human body according to the site, and therefore, it is extremely important to design and produce a shape and a direction of the orientation of the collagen material in ...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K47/42A61K9/00A61K45/06A61L27/54A61L27/24
CPCA61L2300/414A61L2430/16A61L27/24A61K9/0024A61K45/06A61K47/42A61L27/54A61F2/142
Inventor SAKU, TAROISOBE, YOSHIHIROISOBE, TAKAOKI
Owner ATREE
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