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Fermentation process for the production of organic acids

a fermentation process and organic acid technology, applied in the direction of fermentation, etc., can solve the problems of carbon dioxide gas release into the atmosphere, inefficient use of carbon dioxide gas as a source of inorganic carbon in the fermentation solution,

Inactive Publication Date: 2013-05-23
MYRIANT CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The invention relates to a cost-effective way to produce organic acid, such as succinic acid, using an anaerobic fermentation process. The invention replaces the use of expensive K2CO3 and KOH with relatively inexpensive NH4OH, as a source of inorganic carbon in the fermentation process. Additionally, microaeration is used to ensure complete consumption of the organic carbon supply and increase the titer and productivity of succinic acid. This invention can help reduce the cost of production of succinic acid and contribute to global carbon sequestration efforts.

Problems solved by technology

The utilization of carbon dioxide gas as a source of inorganic carbon in the fermentation solution is very inefficient.
Moreover, the continuous pumping of carbon dioxide into the fermentation vessel results in the release of carbon dioxide into the atmosphere.

Method used

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  • Fermentation process for the production of organic acids
  • Fermentation process for the production of organic acids
  • Fermentation process for the production of organic acids

Examples

Experimental program
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Effect test

example 1

Preparation of Solid Inorganic Carbon Source

[0083]A stock solution containing both ammonium hydroxide and ammonium bicarbonate was prepared by means of sequestering carbon dioxide in the solution of ammonium hydroxide (FIG. 1). One liter of 28-30% ammonium hydroxide solution was added to a 3 liter NBS (New Brunswick Scientific) fermentor and carbon dioxide gas was micro-sparged at the rate of 1 L / minute. The ammonium hydroxide solution inside the fermentor was stirred at 500 rpm for an hour. At the end of one hour, the temperature of the fermentor had increased to 39.3° C. from an initial temperature of 19.5° C. Cooling of the fermentor was initiated by circulating cold water through a coil within the fermentor. With the cold water circulation the temperature of the fermentor reached 16.2° C. in about 2 hours. When the solution turned into a white slimy liquid, 325 ml of water was added to obtain approximately 11 M combined solution of ammonium with approximately 3 M bicarbonate. 11...

example 2

Succinic Acid Production with NH4OH and NH4HCO3

[0085]In order to identify the optimal ratio for NH4OH and NH4HCO3 in the fermentative production of succinic acid, a series of succinic acid fermentations were conducted with varying ratios of NH4OH and NH4HCO3. As shown in the Table 1 below, twelve different NH4OH—NH4HCO3 compositions were tested in the succinic acid fermentation using the KJ122 strain of E. coli as a biocatalyst in a total volume of 2,000 ml in a NBS fermentor maintained at 39° C. The fermentation medium also contained KH2PO4 (55 ml of 1M KH2PO4), MgSO4 (4 ml of 1.5 M MgSO4), betaine (4 ml of 1M betaine). and trace elements. KJ122 inoculum had an initial OD550 nm of 6.8 to 7.8 and 150 ml of this inoculum representing 7.5% (v / v) of the total fermentation volume was used. The pH was maintained at 6.5 and fermentation fluid was stirred with the impeller within the fermentor operated at 750 RPM. Glucose solution was fed as required. At the end of the fermentation the ti...

example 3

Potassium Requirement in Succinic Acid Production

[0086]In this study efforts were made to determine whether potassium salts could be entirely eliminated from the fermentation medium without any significant effect on the succinic acid productivity. In the control experiment, the fermentation was carried out with an initial volume of 4,000 ml in AM1 medium using KJ122 as a biocatalyst. 3N NH4OH and 0.75 M K2CO3, and 1.5 N KOH were used as the neutralizing base. Glucose was provided at the initial concentration of 102.9 grams per liter. At the end of 38 hours of fermentation, the glucose was completely utilized. At the end of 38 hours of fermentation, the succinic acid productivity was calculated to be 1.45 g / L / hr. In the second experiment, fermentation was carried out with 6N NH4OH as the only neutralizing base in an initial volume of 2,000 ml. K2CO3 and KOH were completely eliminated from the fermentation medium. Carbon dioxide gas was provided as the source of inorganic carbon at th...

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Abstract

This invention relates to improvements in the fermentation process used in the production of organic acids from biological feedstock using bacterial catalysts. The improvements in the fermentation process involve providing a fermentation medium comprising an appropriate form of inorganic carbon, an appropriate amount of aeration and a biocatalyst with an enhanced ability to uptake and assimilate the inorganic carbon into the organic acids. This invention also provides, as a part of an integrated fermentation facility, a novel process for producing a solid source of inorganic carbon by sequestering carbon released from the fermentation in an alkali solution.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application claims the priority of the U.S. Provisional Application Ser. No. 61 / 400,596, filed on Jul. 31, 2010.GOVERNMENT SUPPORT[0002]The invention was made with United States government support under a contract awarded from the US Department of Energy under Award Number DE-EE0002878 / 001. The United States government has certain rights in the invention.BACKGROUND OF THE INVENTION[0003]A 2004 U.S. Department of Energy report entitled “Top value added chemicals from biomass” has identified twelve building block chemicals that can be produced from renewable feedstocks. The twelve sugar-based building block chemicals are 1,4-diacids (succinic, fumaric and maleic), 2,5-furan dicarboxylic acid, 3-hydroxy propionic acid, aspartic acid, glucaric acid, glutamic acid, itaconic acid, levulinic acid, 3-hydroxybutyrolactone, glycerol, sorbitol, and xylitol / arabinitol.[0004]Building block chemicals are molecules with multiple functional groups t...

Claims

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Application Information

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IPC IPC(8): C12P7/46
CPCC12P7/46
Inventor HERMANN, THERONREINHARDT, JAMESYU, XIAOHUIUDANI, RUSSELLSTAPLES, LAUREN
Owner MYRIANT CORP
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