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Methods for treating metabolic disorders using fgf

a metabolic disorder and metabolic technology, applied in the field of metabolic disorders treated using fgf, can solve the problems of high incidence of type 2 diabetes, high mortality, morbidity and healthcare expenditure, blood vessel and nerve damage, etc., and achieve the effect of high fa

Inactive Publication Date: 2013-05-09
SALK INST FOR BIOLOGICAL STUDIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The patent describes a method for treating metabolic disorders in individuals using an FGF-1 compound. The FGF-1 compound can be administered in various ways, such as through an injection or as a pill. The treatment can help to reduce elevated blood glucose, improve insulin resistance, and treat type II diabetes, obesity, and fatty liver. The method can also involve combining the FGF-1 compound with other therapeutic agents, such as a TZD, to target specific symptoms of the metabolic disorder. The patent also describes a method for inducing fatty liver in food animals by inhibiting FGF-1. Overall, the patent provides a technical solution for using FGF-1 to treat metabolic disorders and associated symptoms.

Problems solved by technology

Metabolic disorders such as type 2 diabetes, obesity, and all of the related complications, are leading causes of mortality.
The incidence of type 2 diabetes is high and rising and is becoming a leading cause of mortality, morbidity, and healthcare expenditure throughout the world (Amos et al., Diabetic Med.
Prolonged high blood sugar may cause blood vessel and nerve damage.
However, there are numerous side effects associated with the use of TZDs, such as weight gain, liver toxicity, cardiovascular toxicity, upper respiratory tract infection, headache, back pain, hyperglycemia, fatigue, sinusitis, diarrhea, hypoglycemia, mild to moderate edema, fluid retention, and anemia (Moller, Nature, 2001, 414: 821-827).

Method used

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  • Methods for treating metabolic disorders using fgf
  • Methods for treating metabolic disorders using fgf
  • Methods for treating metabolic disorders using fgf

Examples

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Effect test

example 1

Identification of FGF-1 as a Direct Target of PPARγ

[0179]To identify nuclear hormone receptor (NHR) targets, we used a “Promoter Ontology” screen, which encompasses a validated cDNA expression library including all 49 mouse NHRs combinatorially paired with a large collection of pathway specific promoter-reporter libraries. The pairing facilitates rapid evaluation of the transcriptional regulation of each genetic pathway by any NHR in a given context. Using this high-throughput promoter screen, we screened promoter constructs for members of the FGF family for regulation by the NHRs, and identified FGF-1 as a direct target of PPARγ. More specifically, we identified strong and specific transcriptional regulation of FGF-1 by PPARγ.

[0180]FGF-1A Promoter Characterization.

[0181]The expression of the FGF-1 gene is directed by at least three distinct promoters driving the untranslated exons: 1A, 1B, and 1D, spaced up to 70 kilobase pairs apart (FIG. 1A) (Myers et al., 1993). Alternative spli...

example 2

FGF-1 Protects Against HFD-Induced Insulin Resistance

[0187]Next, we determined the consequences of loss of FGF-1 in vivo, using FGF-1 knockout (KO) mice. FGF-1 KO mice have been studied in the context of wound healing and cardiovascular changes. Neither these mice, nor FGF-1 / FGF2 double KO mice, displayed any significant phenotype under normal feeding conditions (Miller et al., 2000). To study the role of PPARγ-mediated regulation of FGF-1, FGF-1 KO and wild-type littermates were fed a high fat diet (HFD). Although no difference in HFD-induced weight gain was observed (FIG. 4A), FGF-1 KO mice had smaller WAT and larger, steatotic livers, suggesting that FGF-1 KO mice fail to increase their adipose mass and alternatively mobilize fat into the liver (FIG. 4B, C). At the same time, FGF-1 KO mice displayed increased fasting levels of glucose and insulin and increased insulin resistance compared to wild-type littermates as demonstrated by glucose- and insulin-tolerance tests (GTT, ITT), ...

example 3

AKT Signaling is Impaired in WAT of HFD-fed FGF-1 KO Mice

[0188]FGFs signal through four cognate high-affinity tyrosine kinase receptors, designated FGFR-1 to −4, leading to downstream activation of multiple signal transduction pathways, including the MAPK (ERK1 / 2) and PI3K / AKT pathways. These pathways regulate components of the insulin / glucose signaling pathways including activation of glycogen synthase kinase-3 (GSK-3), which regulates glycogen synthesis in response to insulin, and translocation of the glucose transporter GLUT4 (Cho et al., 2001). To investigate the integrity of these signaling pathways, we determined the expression of its critical components in WAT, BAT, liver, and muscle of HFD-fed FGF-1 KO and wild-type mice (FIG. 5). Interestingly, we found that total levels of AKT (and to a lesser extent GSK3(3) were reduced in WAT of HFD-treated FGF-1 KO mice compared to WT mice. In contrast, levels of AKT were normal in liver, BAT, or muscle, and levels of ERK1 / 2 were normal...

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Abstract

The method provides methods and compositions for treating metabolic disorders such as impaired glucose tolerance, elevated blood glucose, insulin resistance, dyslipidaemia, obesity, and fatty liver.

Description

CROSS-REFERENCES TO RELATED APPLICATIONS[0001]This application claims priority to U.S. Ser. No. 61 / 325,255; U.S. Ser. No. 61 / 325,261; and U.S. Ser. No. 61 / 325,253, all filed Apr. 16, 2010, the disclosures of which are incorporated herein by reference in their entireties.STATEMENT AS TO RIGHTS TO INVENTIONS MADE UNDER FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT[0002]This invention was made with government support under award number LT00305 / 2005-L from the Human Frontier Science Program Organization (HFSPO) as well as grants awarded by the National Institutes of Health (NIH) and the Howard Hughes Medical Institute (HHMI). The Government has certain rights in the invention.BACKGROUND OF THE INVENTION[0003]Metabolic disorders such as type 2 diabetes, obesity, and all of the related complications, are leading causes of mortality. These disorders are associated with the excessive nutritional intake and lack of exercise of the Western lifestyle, and increasingly that of the rest of the wor...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K38/18A61K45/06
CPCA61K9/0019A61K38/1825A61K45/06A61K2300/00A61K47/60A61K47/61A61K31/4439A61P1/16A61P3/00A61P3/04A61P3/06A61P43/00A61P3/10
Inventor JONKER, JOHAN W.DOWNES, MICHAELEVANS, RONALD M.SUH, JAEMYOUNG
Owner SALK INST FOR BIOLOGICAL STUDIES
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