Subtilase Variants

Abstract

The present invention relates to enzymes produced by mutating the genes for a number of subtilases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved stability and / or improved wash performance in any detergent in comparison to their wild type parent enzymes. The enzymes are well-suited for use in any detergent and for some in especially liquid or solid shaped detergent compositions.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application is a continuation of application Ser. No. 10 / 618,934 filed Jul. 14, 2003, which is a continuation of application Ser. No. 09 / 587,747 filed Jun. 5, 2000, now U.S. Pat. No. 6,682,924, which is a continuation of application Ser. No. 09 / 120,577 filed Jul. 22, 1998, now U.S. Pat. No. 6,190,900, which is a continuation of application Ser. No. 08 / 642,987 filed May 6, 1996, now U.S. Pat. No. 5,837,517, which claims priority under 35 U.S.C. 119 of Danish application nos. 0519 / 95 and 0421 / 96 filed May 5, 1995 and Apr. 12, 1996, respectively, and European application no. 95201161 filed May 5, 1995, the contents of which are fully incorporated herein by reference.REFERENCE TO A SEQUENCE LISTING[0002]This application contains a paper copy and computer readable form of a Sequence Listing, which are incorporated herein by reference.BACKGROUND OF THE INVENTION[0003]1. Field of the Invention[0004]This invention relates to novel mutant enz...

AI Technical Summary

Benefits of technology

[0069]It has now surprisingly been found that a subtilase variant having improved storage stability and / or improved performance in detergents, can be obtained by substituting one or more amino acid residues situated in, or in the vicinity of a hydrophobic domain of the parent subtilase for an amino acid residue more hydrophobic than the original residue, said hydrophobic domain comprising the residues corresponding to residues P129, P131, I165, Y167, Y171 of BLS309 (in BASBPN numbering), and said residues in the vicinity thereof comprises residues corresponding to the residues E136, G159, S164, R170, A194, and G195 of BLS309 (in BASBPN numbering), with the exception of the R170M, R170I and R170V variants of BABP92.

[0070]The present invention relates consequently in its first aspect to enzyme variants exhibiting improved stability and / or improved wash performance in detergent.

[0074]Other subtilase gene variants encompassed by the invention are such as those of the subtilase subgroup I-S1, e.g. subtilisin BPN′, and subtilisin Carisberg genes and ensuing variant subtilisin BPN′, Proteinase K, and subtilisin Carlsberg enzymes, which exhibit improved stability in concentrated liquid detergents.

[0075]Still further subtilase gene variants encompassed by the invention are such as Proteinase K and other genes and ensuing variant Proteinase K, and other subtilase enzymes, which exhibit improved stability in concentrated liquid detergents.

Problems solved by technology

Also, it is still not exactly known which physical or chemical characteristics are responsible for a good washing performance or stability of a protease in a specific detergent composition.

Since these positions all except position −1 were known to be involved in the functioning of the enzyme prior to the filing of the application, and therefore evident to select, this application does not contribute much to solving the problem of deciding where to introduce mutations in order to obtain enzymes with desired properties.

However, no data are given in respect of such a variant.

Images