Penicillin g biosensor, systems comprising the same, and measurement using the systems

a biosensor and penicillin technology, applied in the field of biosensors, can solve the problems of easy loss of enzyme activity, high cost and complexity, and easy breakdown and death of cells, and achieve the effects of accurate measurement, low cost, and high sensitivity of ion-sensitive films

Inactive Publication Date: 2009-06-11
CHOU JUNG CHUAN +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Benefits of technology

[0010]The invention provides a penicillin G biosensor comprising an extended gate field effect transistor and a tin oxide sensitive film having a penicillin G acylase film immobil...

Problems solved by technology

This is because PBP cannot supply enough proteins to synthesize bacteria cell walls after penicillin and PBP are combined, finally resulting in breakdown and death of cells.
The chemical methods, however, have several drawbacks, for example, are expensive and complicated, and enzyme activity may easily be l...

Method used

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  • Penicillin g biosensor, systems comprising the same, and measurement using the systems
  • Penicillin g biosensor, systems comprising the same, and measurement using the systems
  • Penicillin g biosensor, systems comprising the same, and measurement using the systems

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Manufacture of the Penicillin G Biosensor

[0037]Referring to FIG. 5, a cross-section of a penicillin G biosensor is illustrated. First, a 0.8 cm×0.5 cm tin oxide film 15 was prepared on an ITO glass 18 to form a sensing unit. The sensing unit was covered by epoxy resin 16, exposing partial tin oxide film 19 to form a sensing window of about 2 mm×2 mm. The sensing unit was connected with a gate of a MOSFET by an aluminum wire 17.

[0038]After the sensing unit and the transistor were packaged, a penicillin G acylase 14 was immobilized on the tin oxide film 15 by gel entrapment to form an enzyme sensor. The preparation of a penicillin G acylase mixing solution is described in the following. First, 80 mg PVA-SbQ (photopolymer, Toyo Gose, Kogyo Company, Japan) was added to 80 μl phosphate buffer solution (pH 7.5) to form IM photopolymer solution. Next, the photopolymer solution (20 mM, pH 7.5) was mixed with penicillin G acylase solution (20 mM, pH 7.5, Sigma Chemical Company, USA) in ratio...

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Abstract

A penicillin G biosensor, systems comprising the same, and measurement using the systems. The penicillin G biosensor has an extended gate field effect transistor (EGFET) structure and comprises a metal oxide semiconductor field effect transistor (MOSFET) on a semiconductor substrate, a sensing unit comprising a substrate, a tin oxide film on the substrate, and a penicillin G acylase film immobilized on the tin oxide film, and a conductive wire connecting the MOSFET and the sensing unit.

Description

CROSS REFERENCE TO RELATED APPLICATIONS[0001]This application is a Divisional of co-pending application Ser. No. 11 / 024,669, filed on Dec. 30, 2004, and for which priority is claimed under 35 U.S.C. § 120; and this application claims priority of Application No. 092137624 filed in Taiwan on Dec. 31, 2003 under 35 U.S.C. § 119; the entire contents of all of which are hereby incorporated by reference.BACKGROUND OF THE INVENTION[0002]1. Field of the Invention[0003]The invention relates to a biosensor, and more specifically to a biosensor measuring penicillin G concentration and systems comprising the same.[0004]2. Brief Discussion of the Related Art[0005]Penicillin is an antibiotic produced from penicillinum. Penicillin binding protein (PBP) is an essential enzyme used in synthesizing bacteria cell walls. When penicillin combines with PBP, synthesis of bacteria cell walls is inhibited. This is because PBP cannot supply enough proteins to synthesize bacteria cell walls after penicillin a...

Claims

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Application Information

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IPC IPC(8): G01N27/26
CPCC12Q1/005G01N33/9446G01N33/54373
Inventor CHOU, JUNG-CHUANYEN, CHIN-HSIENLAI, YI-TING
Owner CHOU JUNG CHUAN
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