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Process of extracting omega-3 polyunsaturated fatty acid from turtle oil

An unsaturated fatty acid and extraction method technology, applied in the field of preparation of active ingredients of medicines, can solve problems such as unsuitable large-scale preparation, easy increase of pollution of eluent, complicated inclusion steps, etc., and achieve stable properties, simple operation and good extraction conditions mild effect

Inactive Publication Date: 2007-06-27
GENERAL HOSPITAL OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are certain defects in the commonly used methods at present. For example, the urea inclusion method needs to be esterified first, the inclusion step is complicated, and carcinogen carbamate can be formed during the inclusion process; the high temperature and long time of the molecular distillation method will lead to polyene Oxidation and decomposition of acid; the eluent in the adsorption separation method is easy to increase pollution, so it is not suitable for large-scale preparation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Put 3.3kg of dry soft-shelled turtle oil into particles with a diameter of 2.0mm and put them into the extraction kettle for supercritical CO 2 Extraction, extraction pressure is 22Mpa, temperature is 40°C, CO 2 The flow rate is 1.8ml / g, the extraction time is 4h, the separation pressure is 8Mpa, and the temperature is 45°C to obtain the extract;

[0026] (2) Acetone was added to the extract obtained in step (1) at a volume ratio of 1:10, stirred for 1 hour, then placed at -60°C for 8 hours, and the supernatant was discarded to obtain 2.16 kg of the extract.

[0027] The instrument used in this example is the HA-221-50-06 supercritical extraction device produced by Jiangsu Huaan Supercritical Extraction Company, and the extraction gas used is CO produced by Beijing Praxair Gas Company. 2 (food grade, 99.99% pure).

Embodiment 2

[0029] (1) After pulverizing 4kg of dry soft-shelled turtle oil, put it into the extraction kettle, and carry out supercritical CO 2 extraction, extraction pressure is 20Mpa, temperature is 50°C, CO 2 The flow rate is 2ml / g, the extraction time is 6h, the separation pressure is 10Mpa, and the temperature is 40°C to obtain the extract;

[0030] (2) Add acetone to the extract obtained in step (1) in a ratio of 1:12 by volume, stir for 1 hour and place it at -50°C for 10 hours, discard the supernatant, and place the precipitate in an oven at 35°C to No acetone smell, 2.99kg of extract was obtained.

[0031] Other conditions are with embodiment 1.

Embodiment 3

[0032] Content determination of EPA and DHA in the extract of embodiment 3

[0033] According to the method of "Measurement of Eicosapentaenoic Acid and Docosahexaenoic Acid in Food" (GB / 75009.168-2003) in "Standards of the People's Republic of China", the content of EPA and DHA in the extract of the present invention is carried out Determination, the specific operation is as follows:

[0034] 1. Preparation of the test solution:

[0035] Get embodiment 2 gained omega-3 polyunsaturated fatty acid extract 1g, after following steps are processed as need testing solution:

[0036] (1), saponification: put the extract in a 50ml stoppered volumetric flask, add n-hexane to make it fully dissolve and make it to 10ml, take 2ml of the resulting solution and place it in a 10ml stoppered test tube, and add 2mol / l potassium hydroxide - Methanol solution 1ml, fully shaken for 10min, heated in a water bath at 60°C for 2min, then cooled to room temperature;

[0037] (2) Methyl esterificat...

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Abstract

The present invention provides process of extracting omega-3 polyunsatuated fatty acid from turtle oil. By means of the combination of supercritical CO2 extraction and low temperature crystallization, the present invention enrichs omega-3 polyunsatuated fatty acid of turtle oil directly. The extraction process of the present invention is simple, short in period and low in power consumption, and the obtained extract has stable property and no solvent pollution and is suitable for use in preparing medicine and nutriment.

Description

【Technical field】 [0001] The invention relates to the preparation of active ingredients of medicines, in particular to the extraction of omega-3 polyunsaturated fatty acids in soft-shelled turtle oil. 【Background technique】 [0002] Soft-shelled turtle is a traditional high-quality Chinese herbal medicine village in my country. It has extremely high medicinal value. It is a good product for nourishing yin and tonifying kidney. Soft-shelled turtle oil is rich in essential fatty acids omega-3 polyunsaturated fatty acids, especially eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), which are known as natural blood purifiers. It has the functions of reducing thrombosis, preventing stroke, preventing arteriosclerosis and myocardial infarction, lowering blood pressure, slowing down inflammation, reducing and improving rheumatoid arthritis, improving and reducing colitis, etc. When the human body has enough omega-3 polyunsaturated fatty acids, it can prevent tumors, ment...

Claims

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Application Information

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IPC IPC(8): C07C57/02C07C51/48A61K35/56A61K35/586
CPCY02P20/54
Inventor 田亚平白晶汪德清邓心新
Owner GENERAL HOSPITAL OF PLA
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